LOCUS       sequence-288062-        9954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 protein; transient expression 
            and generate lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara
            MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake 
            RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak 
            M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, 
            Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch
            F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng
            S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, 
            Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, 
            Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, 
            Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, 
            Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh
            C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, 
            Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, 
            Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J,
            Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, 
            Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura
            N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, 
            Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, 
            Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton 
            J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, 
            Shoichet BK, Krogan NJ
  TITLE     A SARS-CoV-2 protein interaction map reveals targets for drug 
            repurposing.
  JOURNAL   Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. 
            Epub 2020 Apr 30.
  PUBMED    32353859
REFERENCE   2  (bases 1 to 9954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2286-9"; journalName: "Nature"; date: "2020-07";
            volume: "583"; issue: "7816"; pages: "459-468"
COMMENT     Sequence Label: pLVX-EF1alpha-SARS-CoV-2-nsp15-2xStrep-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..9954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          231..1173
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1130..1150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2272..2295
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2332..2355
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    join(2367..2941,2942..2944,2945..2953)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2534..2551)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2761..2780
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2973..3572
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2973..2992)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3469..3489
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3586..4174
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3639..3659)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4057..4068)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4381..5014
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5142..5158)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5142..5158)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5155..5177)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5166..5182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5190..5196,5197..5214,5215..5220))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5235..5256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5373..5390)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5544..6132)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5624..5643)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6303..7094,7095..7163))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6926..6945
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7164..7268)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7316..7450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7353..7372)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7407..7426
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7618..8251
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8298..8423
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8920..9153
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9338..9382
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9645..9760
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        join(9954,1..1182)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
       61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
      961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggatctat ttccggtgaa
     1201 ttcgccgcca ccatgagtct tgaaaacgtc gccttcaacg tagtaaataa gggccacttt
     1261 gatggtcaac agggagaagt acccgtgtct atcatcaata acaccgttta cacgaaggtt
     1321 gacggcgttg atgttgaact gttcgaaaat aagacgacac tcccggtgaa cgtagctttc
     1381 gagctttggg cgaagcgaaa tataaaacct gtccctgaag ttaaaattct caataatctt
     1441 ggggtagaca tcgcggctaa tacagtcata tgggactata agcgggacgc tccggcgcat
     1501 atatctacca tcggagtgtg ctcaatgaca gatattgcca agaaacctac agagacgatt
     1561 tgcgctcctc ttaccgtatt ttttgacggc agggtcgacg gacaagtgga tctgttccga
     1621 aacgcgcgca atggtgttct catcactgag ggtagtgtaa agggactcca gcctagtgtc
     1681 ggacctaagc aagcctcttt gaatggggtc actctgatcg gcgaggctgt taagacgcaa
     1741 tttaactact ataagaaggt cgatggcgtc gtgcagcagc ttcctgaaac atactttaca
     1801 cagtctcgca acttgcaaga atttaaaccg cgctcacaga tggaaataga ctttctcgaa
     1861 ctggcaatgg acgagttcat agagcggtac aaattggaag ggtatgcctt tgaacacatt
     1921 gtttatggtg acttttctca cagccagttg ggtggtttgc atctcctcat agggctggcc
     1981 aaaaggttca aagagagccc ctttgaattg gaggacttca tacctatgga ttcaacagtt
     2041 aagaattact tcattacgga tgcgcagaca ggcagtagta agtgtgtctg ctccgtaatt
     2101 gacctcctct tggacgactt tgtagagata attaagtccc aggatcttag tgtcgtatcc
     2161 aaggtcgtta aggtcacaat tgattacacc gagattagtt ttatgctgtg gtgtaaagac
     2221 ggtcacgtcg agaccttcta tccaaagttg caactcgaag gcggcggggg atggagccat
     2281 ccacaattcg agaaaggcgg tggttcagga ggaggtagcg ggggtggatc atggtcacat
     2341 ccgcagtttg aaaagtaagg atcccgcccc tctccctccc ccccccctaa cgttactggc
     2401 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     2461 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     2521 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     2581 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     2641 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     2701 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     2761 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     2821 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     2881 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     2941 gcttgccaca acccacaagg agacgacctt ccatgaccga gtacaagccc acggtgcgcc
     3001 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
     3061 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
     3121 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     3181 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     3241 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     3301 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     3361 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     3421 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     3481 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     3541 cctggtgcat gacccgcaag cccggtgcct agacgcgtct ggaacaatca acctctggat
     3601 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3661 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3721 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3781 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3841 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3901 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3961 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     4021 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     4081 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     4141 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc
     4201 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     4261 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     4321 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac
     4381 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     4441 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     4501 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     4561 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     4621 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     4681 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     4741 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     4801 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     4861 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     4921 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     4981 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     5041 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag
     5101 cgtttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     5161 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5221 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5281 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     5341 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5401 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5461 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5521 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5581 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5641 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5701 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5761 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5821 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5881 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5941 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6001 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6061 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6121 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6181 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6241 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6301 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6361 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6421 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6481 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6541 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6601 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6661 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6721 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6781 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6841 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6901 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6961 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7021 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7081 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7141 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7201 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7261 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt
     7321 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7381 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7441 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     7501 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     7561 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     7621 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     7681 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     7741 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     7801 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     7861 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     7921 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     7981 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     8041 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     8101 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8161 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8221 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     8281 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     8341 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     8401 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8461 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8521 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     8581 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     8641 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8701 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     8761 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     8821 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     8881 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     8941 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     9001 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     9061 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     9121 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     9181 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     9241 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     9301 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     9361 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     9421 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     9481 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     9541 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     9601 agacagatcc attcgattag tgaacggatc tcgacggtat cgcctttaaa agaaaagggg
     9661 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     9721 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     9781 gcagagatcc agtttatcga tgagtaattc atacaaaagg actcgcccct gccttgggga
     9841 atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg
     9901 ccccctcacc cgcccgctct cgtcatcact gaggtggaga agagcatgcg tgag
//
