LOCUS       sequence-288066-        3666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP6
FEATURES             Location/Qualifiers
     source          1..3666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     111..128
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(287..314)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(406..492)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     541..563
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     555..572
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     556..572
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    588..687
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1564..1663)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1680..1699)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1681..1699)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1704..1720)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1704..1720)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2151..2942
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3035..3623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3524..3543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
       61 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
      121 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
      181 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      241 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
      301 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
      361 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
      421 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
      481 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt
      541 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt
      601 tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa
      661 tgccaacttt gtacaaaaaa gttggcatga gcgccgtgaa gagaaccatc aagggaaccc
      721 accactggct gctgctgacc atcctgacct ccctgctggt gctggtgcag tctacccagt
      781 ggagcctgtt cttcttcctg tacgagaacg ccttcctgcc cttcgctatg ggtatcatcg
      841 ccatgtccgc cttcgctatg atgttcgtga agcacaagca cgctttcctg tgcctgttcc
      901 tgctgccatc cctggccacc gtggcttact tcaacatggt gtacatgccc gcctcttggg
      961 tcatgcgcat catgacctgg ctggacatgg tggacaccag cctgtccggc ttcaagctga
     1021 aggactgcgt gatgtacgcc tctgctgtgg tgctgctgat cctgatgacc gctagaaccg
     1081 tgtacgatga tggtgctaga agggtgtgga ccctgatgaa cgtgctgacc ctggtgtaca
     1141 aggtgtacta cggcaacgcc ctggaccagg ctatcagcat gtgggctctg atcatctctg
     1201 tgaccagcaa ctactccgga gtggtgacca ccgtgatgtt cctggccaga ggtatcgtgt
     1261 tcatgtgcgt ggagtactgc cctatcttct tcatcaccgg caacaccctg cagtgcatca
     1321 tgctggtgta ctgcttcctg ggttacttct gcacctgcta tttcggcctg ttctgcctgc
     1381 tgaacagata cttccgtctg accctgggcg tgtacgacta cctggtgtcc acccaggaat
     1441 tccgttacat gaactctcag ggactgctgc cccctaagaa cagcatcgac gctttcaagc
     1501 tgaacatcaa gctgctggga gtgggcggaa agccatgcat caaggtggcc accgtgcagt
     1561 aatacccaac tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     1621 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     1681 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     1741 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
     1801 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     1861 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     1921 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     1981 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     2041 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     2101 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     2161 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     2221 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     2281 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     2341 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     2401 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     2461 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     2521 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     2581 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     2641 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     2701 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     2761 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     2821 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     2881 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     2941 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     3001 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3061 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3661 tttgct
//
