LOCUS       sequence-288078-        3213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP10 gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP10
FEATURES             Location/Qualifiers
     source          1..3213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     75..91
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     75..91
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     96..115
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        96..114
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    132..231
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(655..754)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(770..787)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(770..786)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(779..801)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      850..936
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1028..1055
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1214..1231)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1385..1973)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1465..1484)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2066..2857)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agaggaggca tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca
       61 cgggccagag ctgccaggaa acagctatga ccatgtaata cgactcacta taggggatat
      121 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
      181 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagttggg tattactgca
      241 gcataggttc cctcagctgg tcgcaagagc atccgtagcc cttccacatt ccgcacacgg
      301 tgcacacggt gttcttcagg gtgaatccca cggggtcgtt agcgcaggtg gttgggatct
      361 gcacgtactt gcccttcagg tcgcagaaac ccttagggtt ggggtggtcg atgtggcatc
      421 tgcagtacag gcagcagctg gcgccaccga aagattcctg gtccatgtta gcctctgggg
      481 tcacggtgat ggcctgaccg gttccggtgt gggtgcacag catcttcacg cagttggtga
      541 taggctgtcc gccggaagcc aggtagtcct tgtaggcctt agcggcgtcc acagcgaagg
      601 cgcagaagga cagcacggtg ctgttagcag gcacctcagt agcgttacca gccatgccaa
      661 cttttttgta caaagttggc attataaaaa agcattgctc atcaatttgt tgcaacgaac
      721 aggtcactat cagtcaaaat aaaatcatta tttggggccc gagcttaaga ctggccgtcg
      781 ttttacaacg tcgtgactgg gaaaacatcc atgctagcgt taacgcgaga gtagggaact
      841 gccaggcatc aaataaaacg aaaggctcag tcggaagact gggcctttcg ttttatctgt
      901 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt
      961 gtgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa
     1021 actaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttcctggc
     1081 tagcggtacg cgtattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     1141 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     1201 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1261 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1321 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1381 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1441 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1501 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1561 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1621 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1681 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1741 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1801 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1861 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1921 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1981 ctttgatctt ttctacgggg tctgacgctc agtggaacga cgcgtaactc acgttaaggg
     2041 attttggtca tgggtggctc gagggttatt tgccgactac cttggtgatc tcgcctttca
     2101 cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc
     2161 caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca
     2221 ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa
     2281 tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc
     2341 atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga
     2401 gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga
     2461 tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct
     2521 gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt
     2581 gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag
     2641 tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg
     2701 taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca
     2761 aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata
     2821 gttgagtcga tacttcggcg atcaccgctt ccctcataat gtttaacttt gttttagggc
     2881 gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
     2941 aacgcgcttg ctgcttggat gcccgaggca tagactgtac cccaaaaaaa cagtcataac
     3001 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc
     3061 agttgcgtga gcgcatacgc tacttgcatt acagcttacg aaccgaacag gcttatgtcc
     3121 actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggtagca
     3181 gcgaagtcga ggcatttctg tcctggctgg tct
//
