LOCUS       sequence-288080-       10830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 protein; transient expression 
            and generate lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara
            MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake 
            RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak 
            M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, 
            Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch
            F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng
            S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, 
            Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, 
            Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, 
            Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, 
            Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh
            C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, 
            Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, 
            Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J,
            Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, 
            Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura
            N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, 
            Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, 
            Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton 
            J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, 
            Shoichet BK, Krogan NJ
  TITLE     A SARS-CoV-2 protein interaction map reveals targets for drug 
            repurposing.
  JOURNAL   Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. 
            Epub 2020 Apr 30.
  PUBMED    32353859
REFERENCE   2  (bases 1 to 10830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2286-9"; journalName: "Nature"; date: "2020-07";
            volume: "583"; issue: "7816"; pages: "459-468"
COMMENT     Sequence Label: pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..10830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2337..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3467..3487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3544..3553
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5485..5508
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5545..5568
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    join(5580..6154,6155..6157,6158..6166)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5747..5764)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5974..5993
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6186..6785
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6186..6205)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6682..6702
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6799..7387
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6852..6872)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7270..7281)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7594..8227
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8355..8371)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8355..8371)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8368..8390)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8379..8395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8403..8409,8410..8427,8428..8433))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8448..8469
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8586..8603)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8757..9345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8837..8856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9516..10307,10308..10376))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10139..10158
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10377..10481)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10529..10663
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10566..10585)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10620..10639
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     3541 gccgccacca tggcctacac gcgatatgtt gacaacaact tttgtggacc tgacggctac
     3601 cctcttgaat gtattaagga tcttctggct cgcgctggaa aagccagttg tacactgtct
     3661 gagcagctcg actttataga tacaaagcgg ggggtctatt gttgccgaga gcatgaacat
     3721 gagatcgcat ggtatacgga gcgctctgaa aaaagctatg aattgcagac accctttgaa
     3781 attaagttgg cgaaaaagtt cgatacattc aatggtgagt gccccaactt tgtctttccc
     3841 cttaacagta tcattaaaac aatccagccc cgggtggaaa agaagaaatt ggatggattc
     3901 atgggcagga tcagaagtgt ttacccagtg gcatccccta atgaatgcaa tcagatgtgc
     3961 ctgtccacgc tgatgaaatg cgaccactgc ggtgaaacgt cttggcaaac gggggacttt
     4021 gtaaaagcaa cgtgcgagtt ttgcggaact gagaacttga caaaggaggg agcgactaca
     4081 tgtggatacc tgcctcagaa cgcggtagtg aaaatatact gtcctgcctg tcacaattct
     4141 gaagttgggc ctgaacatag cttggccgaa taccataacg aaagtggatt gaagacaatt
     4201 cttcgcaaag gaggccggac tatcgcgttc gggggctgcg tgtttagcta tgtaggatgt
     4261 cataataaat gcgcatattg ggttccacga gctagcgcaa acataggctg caaccacaca
     4321 ggcgtagttg gtgagggatc agaaggtctt aatgataact tgctcgaaat attgcaaaag
     4381 gaaaaagtca acataaatat tgtaggagat tttaaattga atgaagagat tgcgataatc
     4441 cttgcgtcat tttccgcaag tacgagtgcg ttcgttgaga ctgtaaaggg gctcgactat
     4501 aaagcgttta agcaaatagt agagagctgc ggaaacttta aagttacgaa gggaaaagcc
     4561 aaaaaaggtg catggaacat tggcgagcag aagagtatac tgtcccccct gtacgccttc
     4621 gcgtcagaag cagctcgagt agttagaagt atattctcac gaacgctcga gacggcgcag
     4681 aatagtgtgc gcgtgctcca gaaggctgct attacgatcc tcgacggcat atcccagtat
     4741 tctcttcgac tgattgatgc tatgatgttc acctccgact tggcgacgaa caatctcgta
     4801 gttatggctt atatcaccgg cggcgttgta caattgacgt ctcagtggct gacgaacatc
     4861 ttcggaaccg tgtacgagaa gctcaaacca gttcttgatt ggcttgaaga aaagtttaag
     4921 gaaggcgtcg agtttctccg ggatgggtgg gaaattgtta agtttatctc cacctgcgcg
     4981 tgtgaaattg tgggagggca aattgtcacc tgcgccaagg aaattaaaga gtcagttcag
     5041 acgtttttta aacttgtgaa caaattcctt gctctctgtg cggatagtat aatcatcggt
     5101 ggggcgaagt tgaaagcact caatcttggt gaaactttcg tgacccacag caaaggcctg
     5161 tataggaagt gtgttaaaag cagggaggag accggtcttc ttatgccgct gaaggcgccc
     5221 aaagagataa tttttcttga gggggaaact cttccgaccg aagtgctcac ggaagaagta
     5281 gttctgaaaa caggggacct tcaaccactg gagcaaccga cctctgaggc agttgaagcg
     5341 ccgctcgtcg gaactcctgt atgtattaac ggcctcatgc tgttggaaat taaggatacg
     5401 gagaagtact gtgcactggc ccccaacatg atggtcacga acaacacctt tacccttaag
     5461 ggcggcctcg aaggcggcgg gggatggagc catccacaat tcgagaaagg cggtggttca
     5521 ggaggaggta gcgggggtgg atcatggtca catccgcagt ttgaaaagta aggatcccgc
     5581 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     5641 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     5701 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     5761 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     5821 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     5881 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     5941 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     6001 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     6061 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     6121 gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccaca aggagacgac
     6181 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     6241 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     6301 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     6361 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     6421 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     6481 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     6541 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     6601 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     6661 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     6721 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     6781 cctagacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6841 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6901 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6961 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7021 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7081 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7141 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     7201 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7261 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7321 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7381 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta
     7441 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     7501 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     7561 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa
     7621 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     7681 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     7741 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     7801 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     7861 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     7921 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     7981 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     8041 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8101 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8161 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8221 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     8281 atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc
     8341 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8401 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8461 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8521 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8581 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8641 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8701 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8761 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8821 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8881 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8941 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9001 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9061 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9121 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9181 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9241 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9301 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9361 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9421 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9481 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9541 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9601 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9661 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9721 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9781 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9841 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9901 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9961 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10021 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10081 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10141 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10201 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10261 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10321 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10381 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10441 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10501 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa
    10561 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    10621 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    10681 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
    10741 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
    10801 ttgttaaata tgtactacaa acttagtagt
//
