LOCUS       sequence-288087-        4377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP14 gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP14
FEATURES             Location/Qualifiers
     source          1..4377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(83..110)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(202..288)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     337..359
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     351..368
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     352..368
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    384..483
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2071..2170)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2187..2206)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2188..2206)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2211..2227)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2211..2227)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2658..3449
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3542..4130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4031..4050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4284..4301
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
       61 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
      121 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
      181 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
      241 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
      301 tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga
      361 cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt
      421 cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      481 gcatggctga gaacgtgacc ggtctgttca aggactgctc taaggtcatc actggtctgc
      541 acccaactca ggctccaacc cacctgtctg tggacaccaa gttcaagacc gaaggactgt
      601 gcgtggacat ccccggtatc cctaaggaca tgacctaccg caggctgatc tccatgatgg
      661 gcttcaagat gaactaccag gtgaacggat accctaacat gttcatcacc agagaggaag
      721 ctatccgcca cgtgagggcc tggatcggtt tcgacgtgga aggttgccac gctaccagag
      781 aagctgtggg taccaacctg ccactgcagc tgggcttctc taccggagtg aacctggtgg
      841 ctgtgcctac cggctacgtg gacaccccaa acaacaccga cttctctaga gtgagcgcca
      901 agccccctcc aggagaccag ttcaagcacc tgatccccct gatgtacaag ggtctgcctt
      961 ggaacgtggt gcgcatcaag atcgtgcaga tgctgtccga caccctgaag aacctgtctg
     1021 acagggtggt gttcgtgctg tgggctcacg gcttcgagct gaccagcatg aagtacttcg
     1081 tgaagatcgg acccgaacgt acctgctgcc tgtgcgacag acgtgccacc tgcttctcca
     1141 ccgcctctga cacctacgct tgctggcacc actccatcgg attcgactac gtgtacaacc
     1201 ctttcatgat cgacgtgcag cagtggggat tcaccggtaa cctgcagtcc aaccacgacc
     1261 tgtactgcca ggtgcacggt aacgcccacg tggcttcttg cgacgctatc atgaccagat
     1321 gcctggccgt gcacgagtgc ttcgtgaagc gtgtggactg gaccatcgag taccctatca
     1381 tcggcgacga actgaagatc aacgccgctt gcagaaaggt gcagcacatg gtggtgaagg
     1441 ccgctctgct ggctgacaag ttcccagtgc tgcacgacat cggaaaccca aaggctatca
     1501 agtgcgtgcc ccaggccgac gtggagtgga agttctacga cgctcagccc tgctccgaca
     1561 aggcctacaa gatcgaggaa ctgttctact cttacgctac ccacagcgac aagttcaccg
     1621 acggcgtgtg cctgttctgg aactgcaacg tggacaggta cccagccaac tctatcgtgt
     1681 gcagattcga cacccgtgtg ctgagcaacc tgaacctgcc cggatgcgac ggcggatccc
     1741 tgtacgtgaa caagcacgcc ttccacaccc ctgctttcga caagtctgcc ttcgtgaacc
     1801 tgaagcagct gcctttcttc tactacagcg actccccatg cgagagccac ggcaagcagg
     1861 tggtgtccga catcgactac gtgcccctga agagcgccac ctgcatcacc cgctgcaacc
     1921 tgggcggcgc cgtgtgcaga caccacgcca acgaatacag gctgtacctg gacgcttaca
     1981 acatgatgat cagcgccggc ttctccctgt gggtgtacaa gcagttcgac acctacaacc
     2041 tgtggaacac cttcaccagg ctgcagtaat acccaacttt cttgtacaaa gttggcatta
     2101 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     2161 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     2221 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     2281 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     2341 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     2401 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     2461 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     2521 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     2581 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     2641 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     2701 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     2761 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     2821 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2881 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2941 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     3001 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     3061 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     3121 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     3181 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     3241 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     3301 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     3361 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     3421 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     3481 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3541 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3601 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3661 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3721 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3781 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3841 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3901 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3961 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4021 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4081 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4141 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4201 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4261 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     4321 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgaca
//
