LOCUS       sequence-288089-        3834 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP15 gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP15
FEATURES             Location/Qualifiers
     source          1..3834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     116..133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(292..319)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(411..497)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     546..568
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     560..577
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     561..577
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..692
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1737..1836)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1853..1872)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1854..1872)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1877..1893)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1877..1893)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2324..3115
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3208..3796
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3697..3716
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
       61 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
      121 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
      181 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      241 caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca
      301 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc
      361 cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta
      421 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga
      481 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat
      541 gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat
      601 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
      661 tataatgcca actttgtaca aaaaagttgg catgtctctg gagaacgtgg ccttcaacgt
      721 ggtgaacaag ggtcacttcg acggtcagca gggcgaagtg cctgtgagca tcatcaacaa
      781 caccgtgtac accaaggtgg acggcgtgga cgtggagctg ttcgaaaaca agaccaccct
      841 gccagtgaac gtggccttcg agctgtgggc taagcgcaac atcaagcccg tgcctgaagt
      901 gaagatcctg aacaacctgg gagtggacat cgccgctaac accgtgatct gggactacaa
      961 gagggacgcc cccgctcaca tcagcaccat cggcgtgtgc tccatgaccg acatcgccaa
     1021 gaagcctacc gagaccatct gcgctccact gaccgtgttc ttcgacggaa gagtggacgg
     1081 tcaggtggac ctgttccgca acgctaggaa cggcgtgctg atcaccgagg gctctgtgaa
     1141 gggactgcag ccaagcgtgg gacccaagca ggcctccctg aacggtgtga ccctgatcgg
     1201 cgaagctgtg aagacccagt tcaactacta caagaaggtg gacggcgtgg tgcagcagct
     1261 gccagagacc tacttcaccc agtctagaaa cctgcaggaa ttcaagcccc gtagccagat
     1321 ggagatcgac ttcctggaac tggccatgga cgagttcatc gaaagataca agctggaggg
     1381 atacgctttc gaacacatcg tgtacggtga cttctctcac agccagctgg gcggactgca
     1441 cctgctgatc ggtctggcca agcgtttcaa ggagtccccc ttcgagctgg aagacttcat
     1501 ccctatggac tctaccgtga agaactactt catcaccgac gctcagaccg gaagctccaa
     1561 gtgcgtgtgc agcgtgatcg acctgctgct ggacgacttc gtggagatca tcaagtccca
     1621 ggacctgtcc gtggtgtcta aggtggtgaa ggtgaccatc gactacaccg agatctcctt
     1681 catgctgtgg tgcaaggacg gccacgtgga aaccttctac cctaagctgc agtaataccc
     1741 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     1801 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     1861 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     1921 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     1981 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     2041 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     2101 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     2161 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     2221 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     2281 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     2341 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     2401 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     2461 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     2521 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     2581 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     2641 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     2701 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     2761 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     2821 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     2881 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     2941 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     3001 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     3061 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     3121 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     3181 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3241 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3301 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3361 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3421 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3481 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3541 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3601 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3661 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3721 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3781 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg cctt
//
