LOCUS       sequence-288092-        3690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP16 gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP16
FEATURES             Location/Qualifiers
     source          1..3690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..183
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(342..369)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(461..547)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     596..618
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     610..627
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     611..627
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    643..742
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1643..1742)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1759..1778)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1760..1778)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1783..1799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1783..1799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2230..3021
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3114..3690,1..12)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3603..3622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
       61 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      121 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      181 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      241 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      301 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
      361 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
      421 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
      481 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
      541 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
      601 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt
      661 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
      721 actttgtaca aaaaagttgg catgtcttcc caggcttggc agcctggagt ggctatgcct
      781 aacctgtaca agatgcagcg tatgctgctg gagaagtgcg acctgcagaa ctacggagac
      841 tccgccaccc tgcccaaggg tatcatgatg aacgtggcta agtacaccca gctgtgccag
      901 tacctgaaca ccctgaccct ggccgtgcct tacaacatgc gcgtgatcca cttcggtgct
      961 ggttctgaca agggagtggc tccaggtacc gctgtgctga gacagtggct gcccaccggt
     1021 accctgctgg tggactctga cctgaacgac ttcgtgtctg atgctgacag caccctgatc
     1081 ggcgactgtg ctactgtgca caccgctaac aagtgggacc tgatcatctc tgacatgtac
     1141 gacccaaaga ccaagaacgt gaccaaggag aacgacagca aggaaggttt cttcacctac
     1201 atctgcggct tcatccagca gaagctggcc ctgggcggat ctgtggctat caagatcacc
     1261 gaacactcct ggaacgctga cctgtacaag ctgatgggac acttcgcctg gtggaccgct
     1321 ttcgtgacca acgtgaacgc ctctagctcc gaggctttcc tgatcggctg caactacctg
     1381 ggaaagcccc gtgaacagat cgacggttac gtgatgcacg ccaactacat cttctggcgc
     1441 aacaccaacc ctatccagct gtctagctac tccctgttcg acatgtctaa gttcccactg
     1501 aagctgagag gcaccgctgt gatgagcctg aaggagggac agatcaacga catgatcctg
     1561 agcctgctgt ccaagggacg cctgatcatc agggaaaaca acagagtggt catctcctct
     1621 gacgtgctgg tgaacaacta atacccaact ttcttgtaca aagttggcat tataagaaag
     1681 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     1741 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     1801 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1861 tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt
     1921 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt
     1981 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     2041 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     2101 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     2161 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     2221 ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     2281 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     2341 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     2401 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     2461 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     2521 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     2581 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     2641 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     2701 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     2761 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     2821 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     2881 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     2941 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
     3001 accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt
     3061 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3121 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3181 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3241 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3301 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3361 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3421 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3481 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3541 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3601 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3661 cgatttttgt gatgctcgtc aggggggcgg
//
