LOCUS       sequence-288097-        4198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of ORF3A gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 ORF3A
FEATURES             Location/Qualifiers
     source          1..4198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(63..149)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    202..301
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1130..1229)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1341..1360)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1419..1438)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2012..2545)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2734..2739,2740..2756,2757..2762))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3419..4007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3908..3927
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4142..4169)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc
       61 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
      121 tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac
      181 gctagcatgg atctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg
      241 ttgcaacaaa ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagttggc
      301 atggacctgt tcatgagaat cttcaccatc ggcaccgtga ccctgaagca gggagagatc
      361 aaggacgcca ccccttctga cttcgtgcgt gccaccgcta ccatcccaat ccaggctagc
      421 ctgcccttcg gatggctgat cgtgggtgtg gccctgctgg ccgtgttcca gtccgcctct
      481 aagatcatca ccctgaagaa gaggtggcag ctggctctgt ccaagggtgt gcacttcgtg
      541 tgcaacctgc tgctgctgtt cgtgaccgtg tactcacacc tgctgctggt ggctgctggt
      601 ctggaggctc cattcctgta cctgtacgct ctggtgtact tcctgcagag catcaacttc
      661 gtgcgcatca tcatgaggct gtggctgtgc tggaagtgca gatccaagaa ccccctgctg
      721 tacgacgcca actacttcct gtgctggcac accaactgct acgactactg catcccctac
      781 aacagcgtga ccagctccat cgtgatcacc tccggtgacg gcaccaccag ccctatctcc
      841 gaacacgact accagatcgg cggatacacc gagaagtggg aatctggagt gaaggactgc
      901 gtggtgctgc actcttactt caccagcgac tactaccagc tgtactccac ccagctgtct
      961 accgacaccg gtgtggagca cgtgaccttc ttcatctaca acaagatcgt ggacgaaccc
     1021 gaggaacacg tgcagatcca caccatcgac gtgtctagcg gcgtggtgaa ccccgtgatg
     1081 gagcctatct acgacgaacc taccaccacc acctccgtgc cactgtaata cccaactttc
     1141 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     1201 actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca
     1261 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     1321 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     1381 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     1441 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     1501 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     1561 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     1621 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     1681 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     1741 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     1801 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     1861 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     1921 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattagg
     1981 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa
     2041 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac
     2101 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga
     2161 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat
     2221 agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc
     2281 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt
     2341 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac
     2401 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg
     2461 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg
     2521 taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
     2581 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa
     2641 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
     2701 cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc
     2761 aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc
     2821 agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg
     2881 catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg
     2941 ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc
     3001 ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag
     3061 gactctagct atagttctag tggttggcta ctaataggtt gtattgatgt tggacgagtc
     3121 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     3181 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     3241 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     3301 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     3361 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3421 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3481 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3541 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3601 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3661 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3721 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3781 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3841 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3901 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3961 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4021 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4081 tatcccctga ttctgtggat aaccgtatta ccgctagcca ggaagagttt gtagaaacgc
     4141 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggc
//
