LOCUS       sequence-288100-        3598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of the envelope (E) 
            gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 E
FEATURES             Location/Qualifiers
     source          1..3598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(52..79)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(171..257)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    310..409
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(638..737)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(849..868)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(927..946)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1520..2053)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2242..2247,2248..2264,2265..2270))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      2927..3515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3416..3435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctgtggat aaccgtatta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca
       61 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc
      121 cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta
      181 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga
      241 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat
      301 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
      361 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagttggcat gtactctttc
      421 gtgagcgagg aaaccggcac cctgatcgtg aactccgtgc tgctgttcct ggccttcgtg
      481 gtgttcctgc tggtgaccct ggctatcctg accgctctga gactgtgcgc ttactgctgc
      541 aacatcgtga acgtgtccct ggtgaagccc tctttctacg tgtacagccg cgtgaagaac
      601 ctgaacagct ccagggtgcc tgacctgctg gtgtaatacc caactttctt gtacaaagtt
      661 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
      721 aataaaatca ttatttgcca tccagctgca gctctggccc gtgtctcaaa atctctgatg
      781 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
      841 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
      901 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
      961 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     1021 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     1081 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     1141 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     1201 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     1261 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     1321 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     1381 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     1441 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaggcc gggaagccga
     1501 tctcggcttg aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt
     1561 cttcccgtat gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt
     1621 gcacgtagat cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg
     1681 cggtggcaat gccctgcctc cggtgctcgc cggagactgc gagatcatag atatagatct
     1741 cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt
     1801 cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat
     1861 cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat
     1921 caagagcagc ccgcatggat ttgacttggt cagggccgag cctacatgtg cgaatgatgc
     1981 ccatacttga gccacctaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc
     2041 tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct
     2101 tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa
     2161 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag
     2221 cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt
     2281 gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag
     2341 gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca
     2401 ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc ctggcttcag
     2461 gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg
     2521 gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagga ctctagctat
     2581 agttctagtg gttggctact aataggttgt attgatgttg gacgagtcgg aatcgcagac
     2641 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     2701 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     2761 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     2821 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagtttt
     2881 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2941 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3001 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3061 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3121 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3181 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3241 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3301 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3361 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3421 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3481 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3541 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctga
//
