LOCUS       sequence-288102-        4039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of the membrane (M) 
            gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 M
FEATURES             Location/Qualifiers
     source          1..4039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(385..412)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(504..590)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    643..742
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1412..1511)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1623..1642)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1701..1720)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2294..2827)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3016..3021,3022..3038,3039..3044))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      join(3701..4039,1..250)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
       61 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      121 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      181 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      241 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      301 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgctag
      361 ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt
      421 tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca
      481 acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
      541 cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca
      601 gttccctact ctcgcgttaa cgctagcatg gatctcgggc cccaaataat gattttattt
      661 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
      721 actttgtaca aaaaagttgg catggctgac tctaacggta ccatcaccgt ggaggaactg
      781 aagaagctgc tggagcagtg gaacctggtc atcggcttcc tgttcctgac ctggatctgc
      841 ctgctgcagt tcgcctacgc taaccgcaac aggttcctgt acatcatcaa gctgatcttc
      901 ctgtggctgc tgtggcctgt gaccctggct tgcttcgtgc tggctgccgt gtaccgcatc
      961 aactggatca ccggcggaat cgccatcgct atggcctgcc tggtgggcct gatgtggctg
     1021 tcttacttca tcgctagctt caggctgttc gccagaaccc gttccatgtg gtctttcaac
     1081 cccgagacca acatcctgct gaacgtgcct ctgcacggaa ccatcctgac cagaccactg
     1141 ctggagagcg aactggtcat cggcgctgtg atcctgagag gacacctgcg tatcgccgga
     1201 caccacctgg gtcgttgcga catcaaggac ctgcccaagg aaatcaccgt ggctaccagc
     1261 cgcaccctgt cctactacaa gctgggagct tctcagagag tggctggtga ctctggtttc
     1321 gctgcttact ctcgctacag gatcggtaac tacaagctga acaccgacca cagctcctct
     1381 agcgacaaca tcgccctgct ggtgcagtaa tacccaactt tcttgtacaa agttggcatt
     1441 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1501 atcattattt gccatccagc tgcagctctg gcccgtgtct caaaatctct gatgttacat
     1561 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     1621 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     1681 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     1741 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     1801 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     1861 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     1921 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     1981 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     2041 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     2101 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     2161 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     2221 tttctcactt gataacctta tttttgacga ggggaaatta ggccgggaag ccgatctcgg
     2281 cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc
     2341 gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt
     2401 agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg
     2461 caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac
     2521 gcggctgctc aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt
     2581 cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt
     2641 ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag
     2701 cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac
     2761 ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc
     2821 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc
     2881 ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc
     2941 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     3001 cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt tcgtgccttc
     3061 atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt
     3121 ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg
     3181 gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgccctggct tcaggagatc
     3241 ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc
     3301 atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc aggactctag ctatagttct
     3361 agtggttggc tactaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     3421 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     3481 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     3541 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     3601 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3661 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3721 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3781 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3841 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3901 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3961 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4021 cggggggttc gtgcacaca
//
