LOCUS       sequence-288123-       10044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 
            fused to MS2 binding protein, and hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10044)
  AUTHORS   Olivero CE, Martinez-Terroba E, Zimmer J, Liao C, Tesfaye E, 
            Hooshdaran N, Schofield JA, Bendor J, Fang D, Simon MD, Zamudio JR, 
            Dimitrova N
  TITLE     p53 Activates the Long Noncoding RNA Pvt1b to Inhibit Myc and 
            Suppress Tumorigenesis.
  JOURNAL   Mol Cell. 2020 Feb 20;77(4):761-774.e8. doi: 
            10.1016/j.molcel.2019.12.014. Epub 2020 Jan 20.
  PUBMED    31973890
REFERENCE   2  (bases 1 to 10044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.12.014"; journalName: "Mol Cell"; date: 
            "2020-02-20- 20"; volume: "77"; issue: "4"; pages: "761-774.e8"
COMMENT     Sequence Label: lenti-SAM-hygro
FEATURES             Location/Qualifiers
     source          1..10044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    285..402
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        495..743
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     495..515
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     666..685
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        781..799
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        851..869
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        1017..2195
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1248..2186
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2143..2163
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2202..2211
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2211..2597
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             2652..2672
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2688..3230
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             3255..3626
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             3642..3695
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3696..4721
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3696..4721
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    4774..5362
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4827..4847)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5245..5256)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5365..5381)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5366..5382)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5891..6071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6133..6721)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6213..6232)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6892..7683,7684..7752))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7515..7534
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7753..7857)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7932..7951)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8123..8502
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8504..8702
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8657..8677
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8720..8900
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8947..9072
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9569..9802
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9987..10031
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
       61 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
      121 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
      181 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
      241 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
      301 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
      361 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
      421 agcagagatc cagtttggtt agtaccgggc ccgggtcgac tagtgatcag tgtgagggag
      481 tgtaaagctg gtttgagggc ctatttccca tgattccttc atatttgcat atacgataca
      541 aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa
      601 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
      661 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
      721 tcttgtggaa aggacgaaac accggagacg ggataccgtc tctgttttag agctaggcca
      781 acatgaggat cacccatgtc tgcagggcct agcaagttaa aataaggcta gtccgttatc
      841 aacttggcca acatgaggat cacccatgtc tgcagggcca agtggcaccg agtcggtgct
      901 ttttttaaac cgctgttcct aggaatcccg aggccttgca aagatggata aagttttaaa
      961 cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct
     1021 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     1081 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     1141 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     1201 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     1261 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     1321 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
     1381 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
     1441 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     1501 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     1561 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     1621 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     1681 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     1741 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     1801 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tgaaggacgc
     1861 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     1921 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     1981 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     2041 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     2101 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     2161 ttcaaagttt ttttcttcca tttcaggtgt cgtgacgtac ggccaccatg gcttcaaact
     2221 ttactcagtt cgtgctcgtg gacaatggtg ggacagggga tgtgacagtg gctccttcta
     2281 atttcgctaa tggggtggca gagtggatca gctccaactc acggagccag gcctacaagg
     2341 tgacatgcag cgtcaggcag tctagtgccc agaagagaaa gtataccatc aaggtggagg
     2401 tccccaaagt ggctacccag acagtgggcg gagtcgaact gcctgtcgcc gcttggaggt
     2461 cctacctgaa catggagctc actatcccaa ttttcgctac caattctgac tgtgaactca
     2521 tcgtgaaggc aatgcagggg ctcctcaaag acggtaatcc tatcccttcc gccatcgccg
     2581 ctaactcagg tatctacagc gctggaggag gtggaagcgg aggaggagga agcggaggag
     2641 gaggtagcgg acctaagaaa aagaggaagg tggcggccgc tggatcccct tcagggcaga
     2701 tcagcaacca ggccctggct ctggccccta gctccgctcc agtgctggcc cagactatgg
     2761 tgccctctag tgctatggtg cctctggccc agccacctgc tccagcccct gtgctgaccc
     2821 caggaccacc ccagtcactg agcgctccag tgcccaagtc tacacaggcc ggcgagggga
     2881 ctctgagtga agctctgctg cacctgcagt tcgacgctga tgaggacctg ggagctctgc
     2941 tggggaacag caccgatccc ggagtgttca cagatctggc ctccgtggac aactctgagt
     3001 ttcagcagct gctgaatcag ggcgtgtcca tgtctcatag tacagccgaa ccaatgctga
     3061 tggagtaccc cgaagccatt acccggctgg tgaccggcag ccagcggccc cccgaccccg
     3121 ctccaactcc cctgggaacc agcggcctgc ctaatgggct gtccggagat gaagacttct
     3181 caagcatcgc tgatatggac tttagtgccc tgctgtcaca gatttcctct agtgggcagg
     3241 gaggaggtgg aagcggcttc agcgtggaca ccagtgccct gctggacctg ttcagcccct
     3301 cggtgaccgt gcccgacatg agcctgcctg accttgacag cagcctggcc agtatccaag
     3361 agctcctgtc tccccaggag ccccccaggc ctcccgaggc agagaacagc agcccggatt
     3421 cagggaagca gctggtgcac tacacagcgc agccgctgtt cctgctggac cccggctccg
     3481 tggacaccgg gagcaacgac ctgccggtgc tgtttgagct gggagagggc tcctacttct
     3541 ccgaagggga cggcttcgcc gaggacccca ccatctccct gctgacaggc tcggagcctc
     3601 ccaaagccaa ggaccccact gtctccgcta gcggcagtgg agagggcaga ggaagtctgc
     3661 taacatgcgg tgacgtcgag gagaatcctg gcccaatgaa aaagcctgaa ctcaccgcga
     3721 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt atccgacctg atgcagctct
     3781 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     3841 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     3901 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
     3961 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     4021 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     4081 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     4141 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     4201 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     4261 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     4321 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     4381 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     4441 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     4501 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     4561 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     4621 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     4681 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gaaacgttgt tgtttggggt
     4741 tgaattactc ttctagaccg cggcgcgccc gataatcaac ctctggatta caaaatttgt
     4801 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4861 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4921 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4981 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5041 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5101 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     5161 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     5221 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     5281 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     5341 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca
     5401 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     5461 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     5521 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5581 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     5641 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     5701 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
     5761 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     5821 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     5881 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5941 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6001 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6061 aatctctagc agcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6121 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6181 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6241 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6301 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6361 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6421 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6481 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6541 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6601 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6661 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6721 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6781 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6841 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6901 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6961 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7021 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7081 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7141 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7201 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7261 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7321 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7381 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7441 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7501 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7561 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7621 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7681 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7741 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7801 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7861 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     7921 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     7981 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     8041 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     8101 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     8161 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     8221 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     8281 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     8341 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     8401 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     8461 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     8521 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     8581 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     8641 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     8701 agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8761 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8821 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8881 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     8941 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     9001 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     9061 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     9121 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     9181 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     9241 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     9301 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     9361 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
     9421 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     9481 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     9541 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     9601 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     9661 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     9721 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     9781 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     9841 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     9901 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     9961 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    10021 gggcaagttt gtggaattgg ttta
//
