LOCUS       sequence-288124-        8472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HIV Env which is isolated a from IRB approved HIV infected 
            patient samples from Singapore and cloned into expression vector 
            (pcDNA 3.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Joshi A, Cox EK, Sedano MJ, Punke EB, Lee RT, Maurer-Stroh S, Kaur 
            P, Ng OT, Garg H
  TITLE     HIV-1 subtype CRF01_AE and B differ in utilization of low levels of 
            CCR5, Maraviroc susceptibility and potential N-glycosylation sites.
  JOURNAL   Virology. 2017 Dec;512:222-233. doi: 10.1016/j.virol.2017.09.026. 
            Epub 2017 Oct 9.
  PUBMED    29020646
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2017-12"; volume: "512:222-233. doi"; pages: " 
            10.1016/j.virol.2017.09.026. Epub 2017 Oct 9"
COMMENT     Sequence Label: pHM140Env
FEATURES             Location/Qualifiers
     source          1..8472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3970..4011
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4021..4038
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4061..4078)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4067..4291
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4337..4765
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4424..4443)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4634..4655
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4774..4794)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4779..5108
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4959..5094
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5021..5040
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5175..5969
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5229..5248)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5839..5858
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6145..6266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6182..6201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6236..6255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6315..6331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6315..6331)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6328..6350)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6339..6355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6363..6369,6370..6387,6388..6393))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6408..6429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6546..6563)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6717..7305)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6797..6816)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7476..8267,8268..8336))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8099..8118
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8337..8441)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccagtac ccttcaccga tcaagcttta ggcatctcct
      961 atggcaggaa gaagcggaaa catcgacgag gaaatcctca aagcagtaaa gatcatcaaa
     1021 atcctctacc aaagcagtaa gtaataagta tatgtaatgt cacctttgga aattagtgca
     1081 atagtaggac tgatagtagc gctaatctta gcaatagtag tgtggactat agtagccata
     1141 gaacttataa aaataagaag agaaaagaaa atagaaaagt tagttaagag aataagagaa
     1201 agagcagaag acagtggaaa tgagagtgag ggggatacag atgaattggc caaacttgtg
     1261 gagatgggag actttgatcc ttgggttggt gataatttgt agtgcctcaa acaacttgtg
     1321 ggtaacagtt tattatgggg ttcctgtgtg gagagatgca gataccaccc tattttgtgc
     1381 atcagatgcc aaagcacatg acccagaagc acacaatatc tgggccacac atgcctgtgt
     1441 gcccacagac cctaacccac aagaaatact cctggaaaat gtaacagaag attttaacat
     1501 gtggaaaaat aaaatggcag agcagatgca ggaggatgta atcagcttat gggatcaaag
     1561 tctaaagcca tgtgtacagt taactcctct ctgtgttact ttacattgtg tcaatgctaa
     1621 tttgagttcg accaacaaca ataacataac caatggcaac ataggaaatc taacagatga
     1681 agtaagaaac tgtactttta atataaccac agaaataaga gataagaagc agcaggttca
     1741 tgcacttttt tataagcttg atatagtatc aattaataaa gctagtagtg agtataggct
     1801 aataaattgt aatacttcag tcattaagca ggcttgtcca aagatatcct ttgatccaat
     1861 ccctatacat tattgtgctc cagctggtta tgcaatttta aagtgtaatg ataagaattt
     1921 caatgggaca gggccatgta aaaatgtcag ctcagtacaa tgcacacatg ggattaagcc
     1981 agtggtatca actcaattgc tgttaaatgg cagtctagca gaagaagaga taataattag
     2041 atctgaaaat ctcacagata atgccaaaac tataatagtg caccttaata aatctgtaga
     2101 gatcaattgt accagacctt ccaacaatac aagaacaagg atgactatag gaccaggaaa
     2161 agtattctat agaacaggag aaataatagg agatataaga aaagcatatt gtgaggttaa
     2221 tggaacaaaa tggaatgaaa ctttaaaaca ggtaattgga aagttaaaag aacactttaa
     2281 taagacaata atctttcaac ccccctcagg aggagatcta gaaattacta tgcatcattt
     2341 taattgtaga ggagaatttt tctattgcaa tacatcacaa ctatttaata catcacaact
     2401 atttaattgt acaaattgta atagcaccat catacttcca tgcaggataa agcaaattat
     2461 aaacatgtgg cagggagtag gacaagcaat gtatgctcct cccatcagtg gaagaattaa
     2521 ttgtgtatca aatattacag gaatactatt gacaagagat ggtggtaata gtagtaataa
     2581 tgagaccttc agacctggag gaggaaatat aaaggacaat tggagaagtg aattatataa
     2641 atataaagta gtagaaattg aaccactagg aatagcaccc actagggcaa agagaagagt
     2701 ggtggagaga gaaaaaagag cagtgggaat aggagctatg atctttgggt tcttaggagc
     2761 agcaggaagc actatgggcg cagcgtcaat aacgctgacg gtacaggcca gacaattatt
     2821 gtctggtata gtgcaacagc aaagcaattt gctgagagct atagaggcgc agcagcatat
     2881 gttgcaactc acagtctggg gcattaaaca gctccaggca agaatcctgg ctgtggaaag
     2941 atacctaaag gatcaaaagt tcctaggact ttggggctgc tctggaaaaa tcatctgcac
     3001 cactaatgtg ccctggaaca gcacttggag taataaatct tatgaagaaa tttggaacaa
     3061 catgacatgg acagaatggg agagagagat tagcaattac acaaaccaga tatataacat
     3121 acttacagag tcgcaaaacc agcaggacag gaatgaaaaa gatttgttag aattggataa
     3181 atgggcaagt ctgtggaatt ggtttgacat aacaagatgg ctgtggtata taaagatatt
     3241 tataatgata gtaggaggtt taataggttt aagaataatt tttgctgtac tttccatagt
     3301 aaatagagtt aggcagggat actcaccttt gtcgttccag atccctaccc atcaccagag
     3361 ggaacacgac aggcccgaaa gaaccgaaga agacggtggc gagcaagaca gagacagatc
     3421 cgtgcgatta gtgaacggat tcttagctct tgtgtgggaa gatctacgga gcctgtgcct
     3481 cttcagctac caccgcttga gagacttaat cttgattgca gcgaggactg tggaacttct
     3541 gggacgcagc agttgcaagg gactgagacg ggggtgggag agcctcaaat atctggggaa
     3601 tcttctgttc tattggggtc aggaattgaa aattagtgct atttctttgt ttaatgctat
     3661 agcaataaca gtagcggggt ggacagatag ggttatagaa gtagcacaag cagctgggag
     3721 agccattctc cacataccta gaagaatcag acagggctta gaaagggctt tgcaataaca
     3781 tgggaggcaa gtggtcaaaa agtagcatag taggatggcc tcagatcagg gaaagaataa
     3841 ggcaaactcc tccagcagca gaaggagtag gagcagtatc tcaagacgga tccagctaag
     3901 ggtcaagaca attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg
     3961 cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt
     4021 catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt
     4081 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4141 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4201 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4261 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     4321 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4381 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4441 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4501 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4561 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4621 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4681 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4741 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     4801 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4861 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4921 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4981 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     5041 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     5101 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     5161 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     5221 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     5281 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     5341 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     5401 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     5461 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     5521 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     5581 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     5641 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5701 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     5761 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5821 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5881 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5941 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgcga aatgaccgac
     6001 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     6061 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     6121 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     6181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6241 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     6301 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6361 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6421 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6481 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6541 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6601 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6661 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6721 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6781 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6841 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6901 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6961 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7021 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7081 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7141 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7201 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7261 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7321 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7381 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7441 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7501 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7561 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7621 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7681 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7741 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7801 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7861 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7921 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7981 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8041 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8101 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8161 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8221 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8281 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8341 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8401 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8461 ccacctgacg tc
//
