LOCUS       sequence-288127-        9782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HIV Env which is isolated a from IRB approved HIV infected 
            patient samples from Singapore and cloned into expression vector 
            (pcDNA 3.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Joshi A, Cox EK, Sedano MJ, Punke EB, Lee RT, Maurer-Stroh S, Kaur 
            P, Ng OT, Garg H
  TITLE     HIV-1 subtype CRF01_AE and B differ in utilization of low levels of 
            CCR5, Maraviroc susceptibility and potential N-glycosylation sites.
  JOURNAL   Virology. 2017 Dec;512:222-233. doi: 10.1016/j.virol.2017.09.026. 
            Epub 2017 Oct 9.
  PUBMED    29020646
REFERENCE   2  (bases 1 to 9782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2017-12"; volume: "512:222-233. doi"; pages: " 
            10.1016/j.virol.2017.09.026. Epub 2017 Oct 9"
COMMENT     Sequence Label: pHM106Env
FEATURES             Location/Qualifiers
     source          1..9782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4818..4844
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             5280..5321
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5331..5348
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5371..5388)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5377..5601
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5647..6075
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5734..5753)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5944..5965
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6084..6104)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6089..6418
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6269..6404
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6331..6350
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6485..7279
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6539..6558)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7149..7168
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7455..7576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7492..7511)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7546..7565
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7625..7641)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7625..7641)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7638..7660)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7649..7665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7673..7679,7680..7697,7698..7703))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7718..7739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7856..7873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8027..8615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8107..8126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8786..9577,9578..9646))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9409..9428
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9647..9751)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccagtac ccttcaccga tcaagcttta ggcatctcct
      961 atggcaggaa gaagcgaaga cagagacgaa gagctcatcc aggcagtaag aacaatcaag
     1021 attctctatc aaagcagtaa gtattaaatg taatgcaaac cttatacgtt ttagcaatag
     1081 tagccttagt actagcagca ttaatagcaa tagttgtgtg gaccatagta tacatagaat
     1141 acaagaaaat attaagacag aaaaaaatag acaggttaat tgatagaata agagaaagag
     1201 cagaagacag tggcaatgag agtgaagggg atcaggaaga actgtcagca tttatggaga
     1261 tggggtatca tgctccttgg aatgttaatg atcagtaatg ctacaaactt gtgggtcaca
     1321 gtctattatg gggtacctgt atggaaagac gcaaccacca ctctattttg tgcatcagat
     1381 gctagggcat atgatacaga ggtacataat atttgggcca cacatgcctg tgtacccaca
     1441 gaccccaacc cacaagaagt agtcttggaa aatgtgacag aaaactttaa catgtggaaa
     1501 aatgacatgg tagaacagat gcaagaagat ataatcagtt tatgggatca aagcctaaag
     1561 ccatgtgtaa aattaacccc actctgtgtt gctatgaatt gcactaatgt gagcagtaat
     1621 actactaata ctaccagtgg tataatggaa ggagtaggag aaataaaaaa ctgctctttc
     1681 aatatcacaa caagcataga aggtatgaag aaaaaacatg caatttttaa tagccttgat
     1741 gtagtaccaa tagataatac cagctatata ctgacgcatt gtaacaccac agtcattaca
     1801 caagcttgtc caaaggtatc ctttgagcca attcccatac attactgtac cccggctggt
     1861 tttgcgcttc taaagtgtaa caataaaacg tacaatggaa caggaccatg tacaaatgtc
     1921 agtacagtac aatgtacaca tggaattagg ccagtagtat caactcaact gctgttaaat
     1981 ggcagtttag cagaagaaga agtaataatt agatctagca acatcatgga caatgctaaa
     2041 gtcataatag tacagctgaa tgaatctgta gaaattaatt gtacaagacc caacaacaat
     2101 acaagaaaga gtatacatct aggactaggg agaagatggt atgcaacaga aataatagga
     2161 gatataagac aagcacattg taaccttagt tccacaaaat ggcagaaaac tttaaaacag
     2221 atagctcaaa agttaagaga acaatttggg aacaaaacaa tagtctttaa tcaatcctca
     2281 ggaggggacc cagagattgt aatgcacagt tttaattgtg ggggggaatt tttctactgc
     2341 aatacaacac aactgtttaa ctctacttgg aatagtactg ggaatattgc agaaaataat
     2401 actgcagaaa atatcacgct tccttgcaga ataaaacaaa ttataaacat gtggcaggga
     2461 gtaggaaaag caatgtatgc ccctcccatt agcggaccaa ttagctgttc atcaaatatt
     2521 acagggttgc tattaacaag agatggtggt aataatgaga cgaacaccac cgagaccttc
     2581 agacctgggg gaggagatat gagggacaat tggagaagtg aattatataa atataaaata
     2641 gtaaaaatag aaccactagg aatagcacct accaaggcaa agagaagagt ggtgcagaga
     2701 gaaaaaagag cagtgggaat gatgggagcg atgttccttg ggttcttggg agcagcagga
     2761 agcactatgg gcgcagcgtc aatggcgctg acggtacagg ccagacaatt gttgtctggt
     2821 atagtgcaac agcagagaaa tctgctgagg gctattgagg cgcaacagca tctgttgcaa
     2881 ctcacagtct ggggcatcaa gcagctccag gcaagagtcc tggctgtgga aagatacctg
     2941 aaggatcaac agctcctagg aatttggggc tgctctggaa aactcatttg caccactgct
     3001 gtgccttgga atgctagttg gagtaataaa tcttacactg acatttggga taatatgact
     3061 tggatggagt gggaaagaga aattgccaat tacacaggag aaatatacaa cttaattgaa
     3121 aaatcgcaga accaacaaga aaagaatgaa ctagaattat tggaattgga ccagtgggca
     3181 agtttgtgga attggtttga cataacaaaa tggctgtggt atataaaaat attcataatg
     3241 atagtaggag gcttggtact gagagatccc ctcataattt ccccaaagcg taaccatgtg
     3301 tgaataaatt ttgagctagt agggttgcag ccacgagtaa gtcttccctt gttattgtgt
     3361 agccagaatg ccgcaaaact tccatgccta agcgaactgt tgagagtacg tttcgatttc
     3421 tgactgtgtt agcctggaag tgcttgtccc aaccttgttt ctgagcatga acgcccgcaa
     3481 gccaacatgt tagttgaagc atcagggcga ttagcagcat gatatcaaaa cgctctgagc
     3541 tgctcgttcg gctatggcgt aggcctagtc cgtaggcagg acttttcaag tctcggaagg
     3601 tttcttcaat ctgcattcgc ttcgaataga tattaacaag ttgtttgggt gttcgaattt
     3661 caacaggtaa gttagttgct agaatccatg gctcctttgc cgacgctgag tagattttag
     3721 gtgacgggtg gtgacaatga gtccgtgtcg agcgctgatt ttttcggcct ttagagcgag
     3781 atttatacaa tagaatttgg catgagattg gattgctttt agtcagcctc ttatagccta
     3841 aagtctttga gtgactagat gacatatcat gtaagttgct gataggtttc cagttttccg
     3901 ctcctaggtc tgcatattgt acttttcctc ttactcgact taaccagtac caacccagct
     3961 tctcaacgga tttataccat ggcactttaa agccagcatc actgacaatg agcggtgtgg
     4021 tgttactcgg tagaatgctc gcaaggtcgg ctagaaattg gtcatgagct ttctttgaac
     4081 attgctctga aagcgggaac gctttctcat aaagagtaac agaacgaccg tgtagtgcga
     4141 ctgaagctcg caataccata agccgttttt gctcacggat atcagaccag tcaacaagta
     4201 caatgggcat cgtattgccc gaacagataa agctagcatg ccaacggtat acagcgagtc
     4261 gctctttgtg gaggtgacga ttacctaaca atcggtcgat tcgtttgatg ttatgttttg
     4321 ttctcgcttt ggttggcagg ttacggccaa gttcggtaag agtgagagtt ttacagtcaa
     4381 gtaaggcgtg gcaagccaac gttaagctgt tgagtcgttt taagtgtaat tcggggcaga
     4441 attggtaaag agagtcgtgt aaaatatcga gttcgcacat tttgttgtct gattattgat
     4501 ttttggcgaa accatttgat catatgacaa gatgtgtatc taccttaact taatgatttt
     4561 gataaaaatc attaggggat tcatcagggc ttggtaggtt taagaatagt ttttgctgtg
     4621 ctttctatag taaatagggt taggcaggga tactcaccat tgtcattgca gacccacttc
     4681 ccagcccaga gggaacacgg caggcccgga ggaatcgaag gagaaggtgg agagcaagac
     4741 agagacagat cagaaccatt tgtgaacgga ttcttgacaa ttatctgggt ggatctacgg
     4801 agcctgtgcc tcttcagcta ccgccacttg agagacttac tcttgattgt aaccaggatt
     4861 gtggaacttc tgggacgcag ggggtgggaa gccctcaagt attggtggaa tctcctccag
     4921 tattggattc aggaactaaa gaatagtgct gttagcttgc tgaatgccac agctatagca
     4981 gtagctgaag ggacagatag gtttatagaa gtagtacaag gagtttatag agctattatc
     5041 cacataccta caagaataag acagggctta gaaagggctt tgctataaga tgggtggcaa
     5101 gtggtcgaaa tgtggtctgg gtggatggcc tagggtaagg gaaagaatgg aacgagctga
     5161 gccagcagca gatggggtgg gagcagtatc tcgagacgga tccagctaag ggtcaagaca
     5221 attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag
     5281 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc
     5341 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     5401 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     5461 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     5521 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     5581 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     5641 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5701 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5761 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5821 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5881 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5941 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6001 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6061 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     6121 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     6181 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6241 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     6301 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     6361 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     6421 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     6481 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     6541 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     6601 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     6661 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     6721 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     6781 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     6841 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     6901 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     6961 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     7021 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     7081 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     7141 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     7201 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     7261 tcttgacgag ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc
     7321 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     7381 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     7441 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7501 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7561 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     7621 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7681 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7741 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7801 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7861 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7921 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7981 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8041 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8101 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8161 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8221 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8281 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8341 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8401 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8461 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8521 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8581 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8641 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8701 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8761 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8821 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8881 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8941 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9001 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9061 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9121 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9181 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9241 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9301 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9361 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9421 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9481 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9541 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9601 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9661 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9721 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9781 tc
//
