LOCUS       sequence-288128-        9235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HIV Env which is isolated a from IRB approved HIV infected 
            patient samples from Singapore and cloned into expression vector 
            (pcDNA 3.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Joshi A, Cox EK, Sedano MJ, Punke EB, Lee RT, Maurer-Stroh S, Kaur 
            P, Ng OT, Garg H
  TITLE     HIV-1 subtype CRF01_AE and B differ in utilization of low levels of 
            CCR5, Maraviroc susceptibility and potential N-glycosylation sites.
  JOURNAL   Virology. 2017 Dec;512:222-233. doi: 10.1016/j.virol.2017.09.026. 
            Epub 2017 Oct 9.
  PUBMED    29020646
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2017-12"; volume: "512:222-233. doi"; pages: " 
            10.1016/j.virol.2017.09.026. Epub 2017 Oct 9"
COMMENT     Sequence Label: pHM075Env
FEATURES             Location/Qualifiers
     source          1..9235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     mobile_element  3250..4017
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             4274..4300
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             4733..4774
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4784..4801
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4824..4841)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4830..5054
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5100..5528
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5187..5206)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5397..5418
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5537..5557)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5542..5871
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5722..5857
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5784..5803
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5938..6732
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5992..6011)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6602..6621
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6908..7029
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6945..6964)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6999..7018
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7078..7094)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7078..7094)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7091..7113)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7102..7118
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7126..7132,7133..7150,7151..7156))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7171..7192
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7309..7326)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7480..8068)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7560..7579)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8239..9030,9031..9099))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8862..8881
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9100..9204)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccagtac ccttcaccga tcaagcttta ggcatctcct
      961 atggcaggaa gaagcgaaga cagagacgaa gagctcatca aggcagtaag gacaatcaag
     1021 cttctctatc aaagcagtaa gtattaaatg taatgcaagc tttaactgtt gtagcaatag
     1081 tagccttagt agtagcagca ttattagcaa tagttgtgtg gaccatagta tacctagaat
     1141 ataagaaatt attaagacag aaaaagatag acaggttaat tgatagaata agagaaagag
     1201 cagaagacag tggcaatgag agtgaagggg atcaggaaga actgtcagca tttatggaga
     1261 tggggtatca tgctccttgg gatgttaatg atgatcaata gtgctacaga aaacttgtgg
     1321 gtcacagtct attatggggt acctgtatgg aaagaagcaa ccaccacttt attttgtgca
     1381 tcagatgcca gagcatatga gacagaggta cataatgttt gggccacaca tgcctgtgta
     1441 cccacagacc ccaacccaca agaagtagtc ttggaaaatg tgacagaaaa ctttaacatg
     1501 tggaaaaatg acatggtaga acagatgcaa gaagatataa tcagtttatg ggatcaaagc
     1561 ctaaagccat gtgtaagatt aaccccactc tgtgttgctt tgaattgcac tgatttgggc
     1621 agtaatacta ctaatttggg caatatttct gccggtagta taatggaagg aggagaaata
     1681 aagaactgct ctttcaatat cacaacaagc ataaaaacta gggtgaaaga acatgcaatt
     1741 tttaatagcc ttgatgtagt accaatagat aataccagct ataggatggt gcattgtaat
     1801 accacagtca ttacacaggt ttgtccaaag gtatcttttg agccaattcc catacattac
     1861 tgtaccccgg ctggttttgc gatcctaaag tgtaacaata aaacattcga tggaacagga
     1921 ccatgtacaa atgtcagtac agtacaatgt acacatggaa ttaggccagt agtatcaact
     1981 caactactgc taaatggcag tttggcagaa gaagaggtaa taattagatc tagcaacttc
     2041 acaaacaatg ctaaagtcat aatagtacag ctgaatgaat ctgtagaaat taattgtaca
     2101 agacccaaca acaatacaag aaagagtata catctaggac cagggaaagc atggtatgca
     2161 acagacataa taggagatat aagacaagca cattgtaacc ttagttacac aaaatggcag
     2221 gacactttaa aaaagatagt tgaaaaatta agaaaacaat ttgggaacaa aacaatagta
     2281 tttaatcaat cctcaggagg ggacccagag attgtaacgc acagttttaa ttgtgggggg
     2341 gaatttttct actgtaatac aacacaactg tttaattcaa cacacctgtt tgatactact
     2401 aatagcactg ggaatgctac aggaaatatc acacttcctt gcagaataaa acaaattgta
     2461 aacatgtggc agggagtagg aaaagcaatg tatgcccctc ccattagagg accaattagc
     2521 tgttcatcaa agattacagg attgctatta acaagagatg gtggtagtaa taatgagaca
     2581 aacaccaccg agaccttcag acctggggga ggagatatga aggacaattg gagaagtgaa
     2641 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacctac caaggcaaag
     2701 agaagagtgg tgcagagaga aaaaagagca gtgggaacaa taggagcgat gttccttggg
     2761 ttcttgggag cagcaggaag cactatgggc gcagcgtcag tgacgctgac ggtacaggcc
     2821 agacaattgt tgtctggtat agtgcaacag cagagaaatt tgctgagagc tattgaggcg
     2881 caacagcata tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagagtcctg
     2941 gctgtggaaa gatacctgaa ggatcaacaa ctcctaggga tttggggctg ctctggaaaa
     3001 ctcatttgca ccactgctgt gccttggaat actagttgga gtaataaatc ttacaatgac
     3061 atttgggaga acatgacttg gatggaatgg gaaagagaaa ttgccaatta cacaggagaa
     3121 atatacacct taattgaaaa atcgcagaac cagcaagaaa agaatgaact agaattattg
     3181 gaattggaca agtgggcaag tttgtggaat tggtttgaca taacaaaatg gctgtggtat
     3241 ataaaaatag gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc
     3301 tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg
     3361 taacggcaaa agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg
     3421 gcaactgcag ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat
     3481 ggccatgaat ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac
     3541 gattttacgt cacttaaaaa actcaggccg cagtcggtaa cctcgcgcat acagccgggc
     3601 agtgacgtca tcgtctgcgc ggaaatggac gaacagtggg gctatgtcgg ggctaaatcg
     3661 cgccagcgct ggctgtttta cgcgtatgac agtctccgga agacggttgt tgcgcacgta
     3721 ttcggtgaac gcactatggc gacgctgggg cgtcttatga gcctgctgtc accctttgac
     3781 gtggtgatat ggatgacgga tggctggccg ctgtatgaat cccgcctgaa gggaaagctg
     3841 cacgtaatca gcaagcgata tacgcagcga attgagcggc ataacctgaa tctgaggcag
     3901 cacctggcac ggctgggacg gaagtcgctg tcgttctcaa aatcggtgga gctgcatgac
     3961 aaagtcatcg ggcattatct gaacataaaa cactatcaat aagttggagt cattacctaa
     4021 aaatattcat aatgatagta ggaggcttgg taggtttaag aatagctttt gctgtacttt
     4081 ctatagtaaa tagggttagg cagggatact caccattgtc attacagacc cgcttcccag
     4141 ctcagaggga acacgacagg cccggaggaa tcggagaaga aggtggagag caagacagag
     4201 acagatccga accattagtg aacggattct tgacaattat ctgggtggat ctacggagcc
     4261 tgtgcctctt cagctaccgc cacttgagag acttactctt aattgtaacc aggattgtgg
     4321 aacttctggg acgcaggggg tgggaagccc tcaagtattg gtggaatctc ctccagtatt
     4381 ggattcagga actaaagagt agtgctgtta gcttgctgaa tgccacagct atagcagtag
     4441 ctgaagggac agatagggtt atagaagtag cacaaagagc tgggagagcc attctcaata
     4501 tacctagaag aatcagacaa ggtttagaaa gggctttgct ataacatggg aaacaagtgg
     4561 tcaaaaacat ggcctcaggt cagagaaaga ataaggcaaa ctcctgtaag gcaaccagca
     4621 gcagaaggag caggagcagt atctcgagac ggatccagct aagggtcaag acaattctgc
     4681 agatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc
     4741 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca
     4801 ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4861 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4921 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4981 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     5041 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     5101 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5161 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5221 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5281 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     5341 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     5401 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     5461 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5521 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     5581 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     5641 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     5701 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5761 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     5821 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     5881 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     5941 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     6001 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     6061 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     6121 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     6181 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     6241 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     6301 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     6361 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     6421 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     6481 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     6541 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     6601 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     6661 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     6721 gagttcttct gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct
     6781 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     6841 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     6901 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6961 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7021 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     7081 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7141 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7201 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7261 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7321 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7381 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7441 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7501 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7561 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7621 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7681 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7741 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7801 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7861 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7921 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7981 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8041 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8101 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8161 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8221 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8281 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8341 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8401 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8461 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8521 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8581 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8641 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8701 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8761 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8821 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8881 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8941 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9001 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9061 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9121 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9181 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
