LOCUS       sequence-288132-        9035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MINPA Gene circuit expression in E. coli. GFP serves as a reporter 
            for Plux/tet promoter activity. mCherry serves as reporter for 
            Para/lac promoter activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9035)
  AUTHORS   Wu F, Su RQ, Lai YC, Wang X
  TITLE     Engineering of a synthetic quadrastable gene network to approach 
            Waddington landscape and cell fate determination.
  JOURNAL   Elife. 2017 Apr 11;6. doi: 10.7554/eLife.23702.
  PUBMED    28397688
REFERENCE   2  (bases 1 to 9035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-11"; volume: "6. doi"; pages: " 10.7554/eLife.23702"
COMMENT     Sequence Label: pSB1A2QS
FEATURES             Location/Qualifiers
     source          1..9035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             77..106
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    314..330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             348..359
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             366..1241
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             1242..1274
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     RBS             1314..1325
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1332..1952
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1405..1424)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             1953..1985
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      2033..2104
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2120..2147
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    2215..2233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2242..2253
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             3049..3060
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3076..4155
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(3095..3114)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             4156..4188
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      4236..4307
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4323..4350
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    4367..4383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4585..4614
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    4822..4838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4856..4867
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4874..5584
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5019..5037)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5287..5306
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      5604..5675
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5691..5718
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    5786..5804
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             5813..5824
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5831..6544
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(5861..5889)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6484..6504
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      6567..6638
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6654..6681
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    6688..6708
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      6709..6766
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(6790..6807)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6961..7549)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7041..7060)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7782..8597)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7782..8597)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8505..8524
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8589..8608
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     8802..8820
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8824..8867)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    8870..8891
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    8894..8910
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggaaaacaat gaaaaggccc ccaaggtagt tatccttaaa aaagccacag catacatcct
       61 gtccgtccaa gcagaggagc aaaagctcat ttctgaagag gacttgttgc ggaaacgacg
      121 agaacagttg aaacacaaac ttgaacagct acggaactct tgtgcgtaag gaaaagtaag
      181 gaaaacgatt ccttctaaca gaaatgtcct gagtcaccta tgaactgtcg attcgagcat
      241 agcattttta tccataagat tagcggatca taatctttac aattttgagc gctcacaatt
      301 atgatagatt cagttgtgag cggataacaa tttcacacat actagagaaa gaggagaaat
      361 actagatggc tgaagcgcaa aatgatcccc tgctgccggg atactcgttt aacgcccatc
      421 tggtggcggg tttaacgccg attgaggcca atggttatct cgattttttt atcgaccgac
      481 cgctgggaat gaaaggttat attctcaatc tcaccattcg cggtcagggg gtggtgaaaa
      541 atcagggacg agaatttgtc tgccgaccgg gtgatatttt gctgttcccg ccaggagaga
      601 ttcatcacta cggtcgtcat ccggaggctc gcgaatggta tcaccagtgg gtttactttc
      661 gtccgcgcgc ctactggcat gaatggctta actggccgtc aatatttgcc aatacgggtt
      721 tctttcgccc ggatgaagcg caccagccgc atttcagcga cctgtttggg caaatcatta
      781 acgccgggca aggggaaggg cgctattcgg agctgctggc gataaatctg cttgagcaat
      841 tgttactgcg gcgcatggaa gcgattaacg agtcgctcca tccaccgatg gataatcggg
      901 tacgcgaggc ttgtcagtac atcagcgatc acctggcaga cagcaatttt gatatcgcca
      961 gcgtcgcaca gcatgtttgc ctgtcgccgt cgcgtctgtc acatcttttc cgccagcagt
     1021 tagggattag cgtcttaagc tggcgcgagg accaacgcat cagccaggcg aagctgcttt
     1081 tgagcactac ccggatgcct atcgccaccg tcggtcgcaa tgttggtttt gacgatcaac
     1141 tctatttctc gcgagtattt aaaaaatgca ccggggccag cccgagcgag ttccgtgccg
     1201 gttgtgaaga aaaagtgaat gatgtagccg tcaagttgtc agctgcaaac gacgaaaact
     1261 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagaaagagg
     1321 agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc
     1381 ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag
     1441 agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca
     1501 ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa agctggcaag
     1561 attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag
     1621 caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat
     1681 tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg
     1741 tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag
     1801 aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct atcgaattat
     1861 ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat
     1921 tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag
     1981 tagcttaata acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa
     2041 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2101 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga
     2161 gacctgtagg atcgtacagg tttacgcaag aaaatggttt gttatagtcg aatatcccta
     2221 tcagtgatag agatactaga gaaagaggag aaatactaga tgaaaaacat aaatgccgac
     2281 gacacataca gaataattaa taaaattaaa gcttgtagaa gcaataatga tattaatcaa
     2341 tgcttatctg atatgactaa aatggtacat tgtgaatatt atttactcgc gatcatttat
     2401 cctcattcta tggttaaatc tgatatttca atcctagata attaccctaa aaaatggagg
     2461 caatattatg atgacgctaa tttaataaaa tatgatccta tagtagatta ttctaactcc
     2521 aatcattcac caattaattg gaatatattt gaaaacaatg ctgtaaataa aaaatctcca
     2581 aatgtaatta aagaagcgaa aacatcaggt cttatcactg ggtttagttt ccctattcat
     2641 acggctaaca atggcttcgg aatgcttagt tttgcacatt cagaaaaaga caactatata
     2701 gatagtttat ttttacatgc gtgtatgaac ataccattaa ttgttccttc tctagttgat
     2761 aattatcgaa aaataaatat agcaaataat aaatcaaaca acgatttaac caaaagagaa
     2821 aaagaatgtt tagcgtgggc atgcgaagga aaaagctctt gggatatttc aaaaatatta
     2881 ggttgcagtg agcgtactgt cactttccat ttaaccaatg cgcaaatgaa actcaataca
     2941 acaaaccgct gccaaagtat ttctaaagca attttaacag gagcaattga ttgcccatac
     3001 tttaaaaatt aataacactg atagtgctag tgtagatcac tactagagaa agaggagaaa
     3061 tactagatgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc
     3121 tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg
     3181 gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa
     3241 ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg
     3301 ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg
     3361 gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc
     3421 gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct
     3481 gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc
     3541 atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc
     3601 gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt
     3661 ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa
     3721 cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag
     3781 ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc
     3841 gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat
     3901 accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg
     3961 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc
     4021 aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa
     4081 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     4141 ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt tagtagctta ataactctga
     4201 tagtgctagt gtagatctct actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     4261 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     4321 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagaattgt gagcggataa
     4381 caaattcaca cagggccctc ggacaccgag gagaatgtca agaggcgaac acacaacgtc
     4441 ttggagcgcc agaggaggaa cgagctaaaa cggagctttt ttgccctgcg tgaccagatc
     4501 ccggagttgg aaaacaatga aaaggccccc aaggtagtta tccttaaaaa agccacagca
     4561 tacatcctgt ccgtccaagc agaggagcaa aagctcattt ctgaagagga cttgttgcgg
     4621 aaacgacgag aacagttgaa acacaaactt gaacagctac ggaactcttg tgcgtaagga
     4681 aaagtaagga aaacgattcc ttctaacaga aatgtcctga gtcacctatg aactgtcgat
     4741 tcgagcatag catttttatc cataagatta gcggatcata atctttacaa ttttgagcgc
     4801 tcacaattat gatagattca gttgtgagcg gataacaatt tcacacatac tagagaaaga
     4861 ggagaaatac tagatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     4921 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     4981 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     5041 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     5101 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     5161 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     5221 ctccttgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     5281 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     5341 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     5401 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     5461 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     5521 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     5581 gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     5641 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     5701 cgggtgggcc tttctgcgtt tatatactag agacctgtag gatcgtacag gtttacgcaa
     5761 gaaaatggtt tgttatagtc gaatatccct atcagtgata gagatactag agaaagagga
     5821 gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
     5881 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     5941 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     6001 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca
     6061 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
     6121 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
     6181 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     6241 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
     6301 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
     6361 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     6421 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     6481 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     6541 caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     6601 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     6661 cttcgggtgg gcctttctgc gtttatatac tagtagcggc cgctgcagtc cggcaaaaaa
     6721 gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat
     6781 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6841 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6901 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6961 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7021 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7081 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7141 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7201 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7261 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7321 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7381 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7441 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7501 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7561 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7621 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7681 atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgagtcccg tcaagtcagc
     7741 gtaatgctct gccagtgtta caaccaatta accaattctg attagaaaaa ctcatcgagc
     7801 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     7861 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     7921 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     7981 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     8041 aaaagcttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     8101 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     8161 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     8221 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     8281 gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     8341 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     8401 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     8461 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     8521 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctggagca agacgtttcc
     8581 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
     8641 gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg
     8701 tggctttgtt gaataaatcg aacttttgct gagttgaagg atcagctcga gtgccacttg
     8761 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     8821 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
     8881 cgcttctaga gaattgtgag cggataacaa attcacacag ggccctcgga caccgaggag
     8941 aatgtcaaga ggcgaacaca caacgtcttg gagcgccaga ggaggaacga gctaaaacgg
     9001 agcttttttg ccctgcgtga ccagatcccg gagtt
//
