LOCUS       sequence-288134-       13431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Base Cas12a CRISPRi plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13431)
  AUTHORS   Knoot CJ, Biswas S, Pakrasi HB
  TITLE     Tunable Repression of Key Photosynthetic Processes Using Cas12a 
            CRISPR Interference in the Fast-Growing Cyanobacterium Synechococcus
            sp. UTEX 2973.
  JOURNAL   ACS Synth Biol. 2020 Jan 17;9(1):132-143. doi: 
            10.1021/acssynbio.9b00417. Epub 2019 Dec 24.
  PUBMED    31829621
REFERENCE   2  (bases 1 to 13431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00417"; journalName: "ACS Synth Biol"; date: 
            "2020-01-17- 17"; volume: "9"; issue: "1"; pages: "132-143"
COMMENT     Sequence Label: pSL3287
FEATURES             Location/Qualifiers
     source          1..13431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    4219..4240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4255..4260,4261..4278,4279..4285)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4293..4309
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4298..4320
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4317..4333
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4317..4333
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4329..4625
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
     primer_bind     4352..4372
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4354..4372
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4419..4438)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4420..4438)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4448..4465)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4448..4464)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4457..4479)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    4705..4724
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(join(4725..4730,4731..4748,4749..4754))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     CDS             complement(4849..5664)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4849..5664)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5572..5591
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5656..5675
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6522..6916
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            7258..7345
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             8584..9555
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             10066..10905
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             10892..11743
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(11997..13079)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDFSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     13041..13060
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(13117..13151)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     protein_bind    13276..13295
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        join(13346..13351,13352..13368,13369..13375)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    13376..13395
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     RBS             13413..13421
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tgtcaattta tcaagaattt gttaataaat atagtttaag taaaactcta agatttgagt
       61 taatcccaca gggtaaaaca cttgaaaaca taaaagcaag aggtttgatt ttagatgatg
      121 agaaaagagc taaagactac aaaaaggcta aacaaataat tgataaatat catcagtttt
      181 ttatagagga gatattaagt tcggtttgta ttagcgaaga tttattacaa aactattctg
      241 atgtttattt taaacttaaa aagagtgatg atgataatct acaaaaagat tttaaaagtg
      301 caaaagatac gataaagaaa caaatatctg aatatataaa ggactcagag aaatttaaga
      361 atttgtttaa tcaaaacctt atcgatgcta aaaaagggca agagtcagat ttaattctat
      421 ggctaaagca atctaaggat aatggtatag aactatttaa agccaatagt gatatcacag
      481 atatagatga ggcgttagaa ataatcaaat cttttaaagg ttggacaact tattttaagg
      541 gttttcatga aaatagaaaa aatgtttata gtagcaatga tattcctaca tctattattt
      601 ataggatagt agatgataat ttgcctaaat ttctagaaaa taaagctaag tatgagagtt
      661 taaaagacaa agctccagaa gctataaact atgaacaaat taaaaaagat ttggcagaag
      721 agctaacctt tgatattgac tacaaaacat ctgaagttaa tcaaagagtt ttttcacttg
      781 atgaagtttt tgagatagca aactttaata attatctaaa tcaaagtggt attactaaat
      841 ttaatactat tattggtggt aaatttgtaa atggtgaaaa tacaaagaga aaaggtataa
      901 atgaatatat aaatctatac tcacagcaaa taaatgataa aacactcaaa aaatataaaa
      961 tgagtgtttt atttaagcaa attttaagtg atacagaatc taaatctttt gtaattgata
     1021 agttagaaga tgatagtgat gtagttacaa cgatgcaaag tttttatgag caaatagcag
     1081 cttttaaaac agtagaagaa aaatctatta aagaaacact atctttatta tttgatgatt
     1141 taaaagctca aaaacttgat ttgagtaaaa tttattttaa aaatgataaa tctcttactg
     1201 atctatcaca acaagttttt gatgattata gtgttattgg tacagcggta ctagaatata
     1261 taactcaaca aatagcacct aaaaatcttg ataaccctag taagaaagag caagaattaa
     1321 tagccaaaaa aactgaaaaa gcaaaatact tatctctaga aactataaag cttgccttag
     1381 aagaatttaa taagcataga gatatagata aacagtgtag gtttgaagaa atacttgcaa
     1441 actttgcggc tattccgatg atatttgatg aaatagctca aaacaaagac aatttggcac
     1501 agatatctat caaatatcaa aatcaaggta aaaaagacct acttcaagct agtgcggaag
     1561 atgatgttaa agctatcaag gatcttttag atcaaactaa taatctctta cataaactaa
     1621 aaatatttca tattagtcag tcagaagata aggcaaatat tttagacaag gatgagcatt
     1681 tttatctagt atttgaggag tgctactttg agctagcgaa tatagtgcct ctttataaca
     1741 aaattagaaa ctatataact caaaagccat atagtgatga gaaatttaag ctcaattttg
     1801 agaactcgac tttggctaat ggttgggata aaaataaaga gcctgacaat acggcaattt
     1861 tatttatcaa agatgataaa tattatctgg gtgtgatgaa taagaaaaat aacaaaatat
     1921 ttgatgataa agctatcaaa gaaaataaag gcgagggtta taaaaaaatt gtttataaac
     1981 ttttacctgg cgcaaataaa atgttaccta aggttttctt ttctgctaaa tctataaaat
     2041 tttataatcc tagtgaagat atacttagaa taagaaatca ttccacacat acaaaaaatg
     2101 gtagtcctca aaaaggatat gaaaaatttg agtttaatat tgaagattgc cgaaaattta
     2161 tagattttta taaacagtct ataagtaagc atccggagtg gaaagatttt ggatttagat
     2221 tttctgatac tcaaagatat aattctatag atgaatttta tagagaagtt gaaaatcaag
     2281 gctacaaact aacttttgaa aatatatcag agagctatat tgatagcgta gttaatcagg
     2341 gtaaattgta cctattccaa atctataata aagatttttc agcttatagc aaagggcgac
     2401 caaatctaca tactttatat tggaaagcgc tgtttgatga gagaaatctt caagatgtgg
     2461 tttataagct aaatggtgag gcagagcttt tttatcgtaa acaatcaata cctaaaaaaa
     2521 tcactcaccc agctaaagag gcaatagcta ataaaaacaa agataatcct aaaaaagaga
     2581 gtgtttttga atatgattta atcaaagata aacgctttac tgaagataag tttttctttc
     2641 actgtcctat tacaatcaat tttaaatcta gtggagctaa taagtttaat gatgaaatca
     2701 atttattgct aaaagaaaaa gcaaatgatg ttcatatatt aagtatagct agaggtgaaa
     2761 gacatttagc ttactatact ttggtagatg gtaaaggcaa tatcatcaaa caagatactt
     2821 tcaacatcat tggtaatgat agaatgaaaa caaactacca tgataagctt gctgcaatag
     2881 agaaagatag ggattcagct aggaaagact ggaaaaagat aaataacatc aaagagatga
     2941 aagagggcta tctatctcag gtagttcatg aaatagctaa gctagttata gagtataatg
     3001 ctattgtggt ttttgaggat ttaaattttg gatttaaaag agggcgtttc aaggtagaga
     3061 agcaggtcta tcaaaagtta gaaaaaatgc taattgagaa actaaactat ctagttttca
     3121 aagataatga gtttgataaa actgggggag tgcttagagc ttatcagcta acagcacctt
     3181 ttgagacttt taaaaagatg ggtaaacaaa caggtattat ctactatgta ccagctggtt
     3241 ttacttcaaa aatttgtcct gtaactggtt ttgtaaatca gttatatcct aagtatgaaa
     3301 gtgtcagcaa atctcaagag ttctttagta agtttgacaa gatttgttat aaccttgata
     3361 agggctattt tgagtttagt tttgattata aaaactttgg tgacaaggct gccaaaggca
     3421 agtggactat agctagcttt gggagtagat tgattaactt tagaaattca gataaaaatc
     3481 ataattggga tactcgagaa gtttatccaa ctaaagagtt ggagaaattg ctaaaagatt
     3541 attctatcga atatgggcat ggcgaatgta tcaaagcagc tatttgcggt gagagcgaca
     3601 aaaagttttt tgctaagcta actagtgtcc taaatactat cttacaaatg cgtaactcaa
     3661 aaacaggtac tgagttagat tatctaattt caccagtagc agatgtaaat ggcaatttct
     3721 ttgattcgcg acaggcgcca aaaaatatgc ctcaagatgc tgatgccaat ggtgcttatc
     3781 atattgggct aaaaggtctg atgctactag gtaggatcaa aaataatcaa gagggcaaaa
     3841 aactcaattt ggttatcaaa aatgaagagt attttgagtt cgtgcagaat aggaataact
     3901 aaggaattcg ggcagtgaaa aaaaaaaccc cgcccctgac agggcggggt tttttttaac
     3961 gtggctttgc gcattaaagc tagacaaaaa aatgaccttc ataaatcgct aatctacaac
     4021 agtagaaatt atttaaagtt cttagaccgg ctactaatac aaaaggctct agacatctac
     4081 aacagtagaa attatttaaa gttcttagac tgaattatct gaaggcacag gaatagtagc
     4141 atctacaaca gtagaaatta tttaaagttc ttagactttt gcaggtgggt ggaaagcggg
     4201 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     4261 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     4321 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     4381 gctggaggac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattacg
     4441 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     4501 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     4561 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     4621 gctgacacct gctctcatct acaacagtag aaattattta aagttcttag acccgttttt
     4681 gcctaaatca gcatcgtacg gcataattgt gagcgctcac aattaacata cgagccggaa
     4741 gcataaagtg taaagtcgac tctagagagg atctgtaaca acaaagccgc cgtcccgtca
     4801 agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc
     4861 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
     4921 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
     4981 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
     5041 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
     5101 gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc
     5161 gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag
     5221 acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg
     5281 caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
     5341 ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg
     5401 gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat
     5461 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
     5521 atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc
     5581 ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga
     5641 cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag
     5701 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
     5761 cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca
     5821 tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc
     5881 cacctacaac aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc
     5941 gattcaggcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgctattc
     6001 tgaccttgcc atcacgactg tgctggtcat taaacgcgta ttcaggctga ccctgcgcgc
     6061 tgcgcagggc tttattgatt ccatttttac actgatgaat gttccgttgc gctgcccgga
     6121 ttacagctga aagcgaccag gtgctcggcg tggcaagact cgcagcgaac ccgtagaaag
     6181 ccatgctcca gccgcccgca ttggagaaat tcttcaaatt cccgttgcac atagcccggc
     6241 aattcctttc cctgctctgc cataagcgca gcgaatgccg ggtaatactc gtcaacgatc
     6301 tgatagagaa gggtttgctc gggtcggtgg ctctggtaac gaccagtatc ccgatcccgg
     6361 ctggccgtcc tggccgccac atgaggcatg ttccgcgtcc ttgcaatact gtgtttacat
     6421 acagtctatc gcttagcgga aagttctttt accctcagcc gaaatgcctg ccgttgctag
     6481 acattgccag ccagtgcccg tcactcccgt actaactgtc acgaacccct gcaataactg
     6541 tcacgccccc ctgcaataac tgtcacgaac ccctgcaata actgtcacgc ccccaaacct
     6601 gcaaacccag caggggcggg ggctggcggg gtgttggaaa aatccatcca tgattatcta
     6661 agaataatcc actaggcgcg gttatcagcg cccttgtggg gcgctgctgc ccttgcccaa
     6721 tatgcccggc cagaggccgg atagctggtc tattcgctgc gctaggctac acaccgcccc
     6781 accgctgcgc ggcaggggga aaggcgggca aagcccgcta aaccccacac caaaccccgc
     6841 agaaatacgc tggagcgctt ttagccgctt tagcggcctt tccccctacc cgaagggtgg
     6901 gggcgcgtgt gcagccccgc agggcctgtc tcggtcgatc attcagcccg gctcatcctt
     6961 ctggcgtggc ggcagaccga acaaggcgcg gtcgtggtcg cgttcaaggt acgcatccat
     7021 tgccgccatg agccgatcct ccggccactc gctgctgttc accttggcca aaatcatggc
     7081 ccccaccagc accttgcgcc ttgtttcgtt cttgcgctct tgctgctgtt cccttgcccg
     7141 cacccgctga atttcggcat tgattcgcgc tcgttgttct tcgagcttgg ccagccgatc
     7201 cgccgccttg ttgctcccct taaccatctt gacaccccat tgttaatgtg ctgtctcgta
     7261 ggctatcatg gaggcacagc ggcggcaatc ccgaccctac tttgtagggg agggcgcact
     7321 taccggtttc tcttcgagaa actggcctaa cggccaccct tcgggcggtg cgctctccga
     7381 gggccattgc atggagccga aaagcaaaag caacagcgag gcagcatggc gatttatcac
     7441 cttacggcga aaaccggcag caggtcgggc ggccaatcgg ccagggccaa ggccgactac
     7501 atccagcgcg aaggcaagta tgcccgcgac atggatgaag tcttgcacgc cgaatccggg
     7561 cacatgccgg agttcgtcga gcggcccgcc gactactggg atgctgccga cctgtatgaa
     7621 cgcgccaatg ggcggctgtt caaggaggtc gaatttgccc tgccggtcga gctgaccctc
     7681 gaccagcaga aggcgctggc gtccgagttc gcccagcacc tgaccggtgc cgagcgcctg
     7741 ccgtatacgc tggccatcca tgccggtggc ggcgagaacc cgcactgcca cctgatgatc
     7801 tccgagcgga tcaatgacgg catcgagcgg cccgccgctc agtggttcaa gcggtacaac
     7861 ggcaagaccc cggagaaggg cggggcacag aagaccgaag cgctcaagcc caaggcatgg
     7921 cttgagcaga cccgcgaggc atgggccgac catgccaacc gggcattaga gcgggctggc
     7981 cacgacgccc gcattgacca cagaacactt gaggcgcagg gcatcgagcg cctgcccggt
     8041 gttcacctgg ggccgaacgt ggtggagatg gaaggccggg gcatccgcac cgaccgggca
     8101 gacgtggccc tgaacatcga caccgccaac gcccagatca tcgacttaca ggaataccgg
     8161 gaggcaatag accatgaacg caatcgacag agtgaagaaa tccagaggca tcaacgagtt
     8221 agcggagcag atcgaaccgc tggcccagag catggcgaca ctggccgacg aagcccggca
     8281 ggtcatgagc cagacccagc aggccagcga ggcgcaggcg gcggagtggc tgaaagccca
     8341 gcgccagaca ggggcggcat gggtggagct ggccaaagag ttgcgggagg tagccgccga
     8401 ggtgagcagc gccgcgcaga gcgcccggag cgcgtcgcgg gggtggcact ggaagctatg
     8461 gctaaccgtg atgctggctt ccatgatgcc tacggtggtg ctgctgatcg catcgttgct
     8521 cttgctcgac ctgacgccac tgacaaccga ggacggctcg atctggctgc gcttggtggc
     8581 ccgatgaaga acgacaggac tttgcaggcc ataggccgac agctcaaggc catgggctgt
     8641 gagcgcttcg atatcggcgt cagggacgcc accaccggcc agatgatgaa ccgggaatgg
     8701 tcagccgccg aagtgctcca gaacacgcca tggctcaagc ggatgaatgc ccagggcaat
     8761 gacgtgtata tcaggcccgc cgagcaggag cggcatggtc tggtgctggt ggacgacctc
     8821 agcgagtttg acctggatga catgaaagcc gagggccggg agcctgccct ggtagtggaa
     8881 accagcccga agaactatca ggcatgggtc aaggtggccg acgccgcagg cggtgaactt
     8941 cgggggcaga ttgcccggac gctggccagc gagtacgacg ccgacccggc cagcgccgac
     9001 agccgccact atggccgctt ggcgggcttc accaaccgca aggacaagca caccacccgc
     9061 gccggttatc agccgtgggt gctgctgcgt gaatccaagg gcaagaccgc caccgctggc
     9121 ccggcgctgg tgcagcaggc tggccagcag atcgagcagg cccagcggca gcaggagaag
     9181 gcccgcaggc tggccagcct cgaactgccc gagcggcagc ttagccgcca ccggcgcacg
     9241 gcgctggacg agtaccgcag cgagatggcc gggctggtca agcgcttcgg tgatgacctc
     9301 agcaagtgcg actttatcgc cgcgcagaag ctggccagcc ggggccgcag tgccgaggaa
     9361 atcggcaagg ccatggccga ggccagccca gcgctggcag agcgcaagcc cggccacgaa
     9421 gcggattaca tcgagcgcac cgtcagcaag gtcatgggtc tgcccagcgt ccagcttgcg
     9481 cgggccgagc tggcacgggc accggcaccc cgccagcgag gcatggacag gggcgggcca
     9541 gatttcagca tgtagtgctt gcgttggtac tcacgcctgt tatactatga gtactcacgc
     9601 acagaagggg gttttatgga atacgaaaaa agcgcttcag ggtcggtcta cctgatcaaa
     9661 agtgacaagg gctattggtt gcccggtggc tttggttata cgtcaaacaa ggccgaggct
     9721 ggccgctttt cagtcgctga tatggccagc cttaaccttg acggctgcac cttgtccttg
     9781 ttccgcgaag acaagccttt cggccccggc aagtttctcg gtgactgata tgaaagacca
     9841 aaaggacaag cagaccggcg acctgctggc cagccctgac gctgtacgcc aagcgcgata
     9901 tgccgagcgc atgaaggcca aagggatgcg tcagcgcaag ttctggctga ccgacgacga
     9961 atacgaggcg ctgcgcgagt gcctggaaga actcagagcg gcgcagggcg ggggtagtga
    10021 ccccgccagc gcctaaccac caactgcctg caaaggaggc aatcaatggc tacccataag
    10081 cctatcaata ttctggaggc gttcgcagca gcgccgccac cgctggacta cgttttgccc
    10141 aacatggtgg ccggtacggt cggggcgctg gtgtcgcccg gtggtgccgg taaatccatg
    10201 ctggccctgc aactggccgc acagattgca ggcgggccgg atctgctgga ggtgggcgaa
    10261 ctgcccaccg gcccggtgat ctacctgccc gccgaagacc cgcccaccgc cattcatcac
    10321 cgcctgcacg cccttggggc gcacctcagc gccgaggaac ggcaagccgt ggctgacggc
    10381 ctgctgatcc agccgctgat cggcagcctg cccaacatca tggccccgga gtggttcgac
    10441 ggcctcaagc gcgccgccga gggccgccgc ctgatggtgc tggacacgct gcgccggttc
    10501 cacatcgagg aagaaaacgc cagcggcccc atggcccagg tcatcggtcg catggaggcc
    10561 atcgccgccg ataccgggtg ctctatcgtg ttcctgcacc atgccagcaa gggcgcggcc
    10621 atgatgggcg caggcgacca gcagcaggcc agccggggca gctcggtact ggtcgataac
    10681 atccgctggc agtcctacct gtcgagcatg accagcgccg aggccgagga atggggtgtg
    10741 gacgacgacc agcgccggtt cttcgtccgc ttcggtgtga gcaaggccaa ctatggcgca
    10801 ccgttcgctg atcggtggtt caggcggcat gacggcgggg tgctcaagcc cgccgtgctg
    10861 gagaggcagc gcaagagcaa gggggtgccc cgtggtgaag cctaagaaca agcacagcct
    10921 cagccacgtc cggcacgacc cggcgcactg tctggccccc ggcctgttcc gtgccctcaa
    10981 gcggggcgag cgcaagcgca gcaagctgga cgtgacgtat gactacggcg acggcaagcg
    11041 gatcgagttc agcggcccgg agccgctggg cgctgatgat ctgcgcatcc tgcaagggct
    11101 ggtggccatg gctgggccta atggcctagt gcttggcccg gaacccaaga ccgaaggcgg
    11161 acggcagctc cggctgttcc tggaacccaa gtgggaggcc gtcaccgctg atgccatggt
    11221 ggtcaaaggt agctatcggg cgctggcaaa ggaaatcggg gcagaggtcg atagtggtgg
    11281 ggcgctcaag cacatacagg actgcatcga gcgcctttgg aaggtatcca tcatcgccca
    11341 gaatggccgc aagcggcagg ggtttcggct gctgtcggag tacgccagcg acgaggcgga
    11401 cgggcgcctg tacgtggccc tgaacccctt gatcgcgcag gccgtcatgg gtggcggcca
    11461 gcatgtgcgc atcagcatgg acgaggtgcg ggcgctggac agcgaaaccg cccgcctgct
    11521 gcaccagcgg ctgtgtggct ggatcgaccc cggcaaaacc ggcaaggctt ccatagatac
    11581 cttgtgcggc tatgtctggc cgtcagaggc cagtggttcg accatgcgca agcgccgcca
    11641 gcgggtgcgc gaggcgttgc cggagctggt cgcgctgggc tggacggtaa ccgagttcgc
    11701 ggcgggcaag tacgacatca cccggcccaa ggcggcaggc tgaccccccc cactctattg
    11761 taaacaagac atttttatct tttatattca atggcttatt ttcctgctaa ttggtaatac
    11821 catgaaaaat accatgctca gaaaaggctt aacaatattt tgaaaaattg cctactgagc
    11881 gctgccgcac agctccatag gccgctttcc tggctttgct tccagatgta tgctcttctg
    11941 ctcctgcaag gtcgaattca ggtaccatgt ctagctttaa tgcggtagtt agatcttcac
    12001 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    12061 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
    12121 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
    12181 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
    12241 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
    12301 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
    12361 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcacta
    12421 aagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
    12481 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
    12541 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
    12601 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
    12661 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
    12721 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
    12781 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
    12841 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
    12901 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
    12961 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
    13021 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
    13081 ttcaccaccc tgaattgact ctcttccagt atagatgcta gcattatacc taggactgag
    13141 ctagctgtca agaattcctt agttattcct attctgcacg aactcaaaat actcttcatt
    13201 tttgataacc aaattgagtt ttttgccctc ttgattattt ttgatcctac ctagtagcct
    13261 cttctgctcc tgcagaattg tgagcgctca caattgatat tttggtctgt cgttgcgatc
    13321 gcccgttgca ggccgacatg aaggattgac aattaatcat ccggctcgta taatgaattg
    13381 tgagcgctca caattggtac cggtgatacc gctaaggagg taagtgcacc a
//
