LOCUS       sequence-288141-        9030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector to knockin  tdTomado  into EFNB2 allele.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Zhang J, Chu LF, Hou Z, Schwartz MP, Hacker T, Vickerman V, Swanson 
            S, Leng N, Nguyen BK, Elwell A, Bolin J, Brown ME, Stewart R, 
            Burlingham WJ, Murphy WL, Thomson JA
  TITLE     Functional characterization of human pluripotent stem cell-derived 
            arterial endothelial cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jul 25;114(30):E6072-E6078. doi: 
            10.1073/pnas.1702295114. Epub 2017 Jul 10.
  PUBMED    28696312
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1702295114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-07-25- 25"; volume: "114"; issue: "30"; pages: 
            "E6072-E6078"
COMMENT     Sequence Label: EFNB2-tdTomato
FEATURES             Location/Qualifiers
     source          1..9030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      519..528
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             525..1955
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    2077..2198
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2114..2133)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2168..2187
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(2210..2243)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        2262..2761
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2297..2320)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2694..2713
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        2778..2825
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2844..3647
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2907..2926)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3517..3536
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(3786..3819)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      complement(4403..4991)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4483..4502)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5162..5953,5954..6022))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5785..5804
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6023..6127)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(join(6195..7529,7530..7616))
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYKVPEFDSSTIKNISSAKGLDVWDSWPLQNTDGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(7617..8062)
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     protein_bind    8077..8110
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             8139..8798
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8205..8224)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      8819..8913
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(8949..8984)
                     /codon_start=1
                     /product="6x(His-Asn) affinity tag"
                     /label=6xHN
                     /translation="HNHNHNHNHNHN"
ORIGIN
        1 gcatgcctgc aggtcgactt atttttcaca tttttaaggg tcataaaagc agtccgtctg
       61 cactgggaca gcagtaacta tctctgacct tttctgtctc cgcgtctgca ggttctagca
      121 cagacggcaa cagcgccgga cattcgggga acaacatcct cggttccgaa gtggccttat
      181 ttgcagggat tgcttcagga tgcatcatct tcatcgtcat catcatcacg ctggtggtcc
      241 tcttgctgaa gtaccggagg agacacagga agcactcgcc gcagcacacg accacgctgt
      301 cgctcagcac actggccaca cccaagcgca gcggcaacaa caacggctca gagcccagtg
      361 acattatcat cccgctaagg actgcggaca gcgtcttctg ccctcactac gagaaggtca
      421 gcggcgacta cgggcacccg gtgtacatcg tccaggagat gcccccgcag agcccggcga
      481 acatttacta caaggtcttg gatccccggg taccggtcgc caccatggtg agcaagggcg
      541 aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc
      601 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      661 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
      721 tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc
      781 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg
      841 tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc
      901 gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg
      961 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc
     1021 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga
     1081 agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca
     1141 acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc
     1201 tggggcatgg caccggcagc accggcagcg gcagctccgg caccgcctcc tccgaggaca
     1261 acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga
     1321 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     1381 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
     1441 cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca
     1501 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     1561 gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga
     1621 agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct
     1681 gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc
     1741 aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg
     1801 ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct
     1861 cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc
     1921 tgttcctgta cggcatggac gagctgtaca agtaggcggc cgcgactcta gatcataatc
     1981 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     2041 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     2101 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     2161 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgagatctg aagttcctat
     2221 actttctaga gaataggaac ttcacgaagt tatattctac cgggtagggg aggcgctttt
     2281 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     2341 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     2401 tggtggcccc gtcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     2461 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     2521 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     2581 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     2641 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     2701 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     2761 gacctgcagc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     2821 acgacaaggt gaggaactaa accatgggat cggccattga acaagatgga ttgcacgcag
     2881 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacgatcg
     2941 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     3001 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     3061 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     3121 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     3181 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     3241 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     3301 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     3361 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     3421 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     3481 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     3541 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgcccccg
     3601 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     3661 gttcgaataa agaccgacca agcgacgtct gagagctccc tggcgaattc ggtaccaata
     3721 aaagagcttt attttcatga tctgtgtgtt ggtttttgtg tgcggcgcga taacttcgta
     3781 tagctgaagt tcctatactt tctagagaat aggaacttcg ctagctgaga gggaccctgg
     3841 tggtacctgt gctttcccag aggacaccta atgtcccgat gcctcccttg agggtttgag
     3901 agcccgcgtg ctggagaatt gactgaagca cagcaccggg ggagagggac actcctcctc
     3961 ggaagagccc gtcgcgctgg acagcttacc tagtcttgta gcattcggcc ttggtgaaca
     4021 cacacgctcc ctggaagctg gaagactgtg cagaagacgc ccattcggac tgctgtgccg
     4081 cgtcccacgt ctcctcctcg aagccatgtg ctgcggtcac tcaggcctct gcagaagcca
     4141 agggaagaca gtggtttgtg gacgagaggg ctgtgagcat cctggcaggt gccccaggat
     4201 gccacgcctg gaagggccgg cttctgcctg gggtgcattt cccccgcagt gcataccgga
     4261 cttgtcacac ggacctcggg ctagttacgc gtaaggcggt aatacggtta tccacagaat
     4321 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4381 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4441 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4501 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4561 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4621 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4681 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4741 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4801 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4861 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4921 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4981 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5041 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5101 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5161 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5221 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5281 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5341 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5401 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5461 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5521 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5581 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5641 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5701 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5761 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5821 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5881 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5941 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6001 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6061 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6121 ttccgcgcac atttccccga aaagtgccac ctgacgtcaa tgccaatagg atatcggcat
     6181 tttcttttgc gtttttattt gttaactgtt aattgtcctt gttcaaggat gctgtctttg
     6241 acaacagatg ttttcttgcc tttgatgttc agcaggaagc taggcgcaaa cgttgattgt
     6301 ttgtctgcgt agaatcctct gtttgtcata tagcttgtaa tcacgacatt gtttcctttc
     6361 gcttgaggta cagcgaagtg tgagtaagta aaggttacat cgttaggatc aagatccatt
     6421 tttaacacaa ggccagtttt gttcagcggc ttgtatgggc cagttaaaga attagaaaca
     6481 taaccaagca tgtaaatatc gttagacgta atgccgtcaa tcgtcatttt tgatccgcgg
     6541 gagtcagtga acaggtacca tttgccgttc attttaaaga cgttcgcgcg ttcaatttca
     6601 tctgttactg tgttagatgc aatcagcggt ttcatcactt ttttcagtgt gtaatcatcg
     6661 tttagctcaa tcataccgag agcgccgttt gctaactcag ccgtgcgttt tttatcgctt
     6721 tgcagaagtt tttgactttc ttgacggaag aatgatgtgc ttttgccata gtatgctttg
     6781 ttaaataaag attcttcgcc ttggtagcca tcttcagttc cagtgtttgc ttcaaatact
     6841 aagtatttgt ggcctttatc ttctacgtag tgaggatctc tcagcgtatg gttgtcgcct
     6901 gagctgtagt tgccttcatc gatgaactgc tgtacatttt gatacgtttt tccgtcaccg
     6961 tcaaagattg atttataatc ctctacaccg ttgatgttca aagagctgtc tgatgctgat
     7021 acgttaactt gtgcagttgt cagtgtttgt ttgccgtaat gtttaccgga gaaatcagtg
     7081 tagaataaac ggatttttcc gtcagatgta aatgtggctg aacctgacca ttcttgtgtt
     7141 tggtctttta ggatagaatc atttgcatcg aatttgtcgc tgtctttaaa gacgcggcca
     7201 gcgtttttcc agctgtcaat agaagtttcg ccgacttttt gatagaacat gtaaatcgat
     7261 gtgtcatccg catttttagg atctccggct aatgcaaaga cgatgtggta gccgtgatag
     7321 tttgcgacag tgccgtcagt gttttgtaat ggccagctgt cccaaacgtc caggcctttt
     7381 gcagaagaga tatttttaat tgtggacgaa tcgaactcag gaactttata tttttcattt
     7441 ttttgctgtt cagggatttg cagcatatca tggcgtgtaa tatgggaaat gccgtatgtt
     7501 tccttatatg gcttttggtt cgtttctttc gcaaacgctt gagttgcgcc tcctgccagc
     7561 agtgcggtag taaaggttaa tactgttgct tgttttgcaa actttttgat gttcatcgtt
     7621 catgtctcct tttttatgta ctgtgttagc ggtctgcttc ttccagccct cctgtttgaa
     7681 gatggcaagt tagttacgca caataaaaaa agacctaaaa tatgtaaggg gtgacgccaa
     7741 agtatacact ttgcccttta cacattttag gtcttgcctg ctttatcagt aacaaacccg
     7801 cgcgatttac ttttcgacct cattctatta gactctcgtt tggattgcaa ctggtctatt
     7861 ttcctctttt gtttgataga aaatcataaa aggatttgca gactacgggc ctaaagaact
     7921 aaaaaatcta tctgtttctt ttcattctct gtatttttta tagtttctgt tgcatgggca
     7981 taaagttgcc tttttaatca caattcagaa aatatcataa tatctcattt cactaaataa
     8041 tagtgaacgg caggtatatg tggacgtcgc ggccgcataa cttcgtataa tgtatgctat
     8101 acgaagttat ggatctttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     8161 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     8221 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     8281 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     8341 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg
     8401 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     8461 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     8521 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     8581 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     8641 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     8701 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     8761 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tgatatcgag ctcgcttgga
     8821 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
     8881 ggttgccgcc gggcgttttt tattggtgag aatccaagca ctagtcatga ccaggatctc
     8941 ctaggctagt tgtgattatg attatgatta tgattatgat tatgaccact tacctgggcc
     9001 cgcgcgccaa acgaatggtc tagaaagctt
//
