LOCUS       sequence-288178-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for sACE2-sfGFP high affinity variant 
            2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert 
            AS, Procko E
  TITLE     Engineering human ACE2 to optimize binding to the spike protein of 
            SARS coronavirus 2.
  JOURNAL   Science. 2020 Sep 4;369(6508):1261-1265. doi: 
            10.1126/science.abc0870. Epub 2020 Aug 4.
  PUBMED    32753553
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc0870"; journalName: "Science"; date: "2020-09-4-
            4"; volume: "369"; issue: "6508"; pages: "1261-1265"
COMMENT     Sequence Label: pcDNA3-sACE2v2-sfGFP
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..731)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             complement(21..731)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(61..81)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     676..704
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cctctagact cgagttatca tttgtagagc tcatccatgc catgtgtaat cccagcagca
       61 gttacaaact caagaaggac catgtggtca cgcttttcgt tgggatcttt cgaaaggaca
      121 gattgtgtcg acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta
      181 ttttgttgat aatggtctgc tagttgaacg gaaccatctt caacgttgtg gcgaattttg
      241 aagttagctt tgattccatt cttttgtttg tctgccgtga tgtatacatt gtgtgagtta
      301 aagttgtact ccagtttgtg tccgagaatg tttccatctt ctttaaaatc aatacctttt
      361 aactcgatac gattaacaag ggtatcacct tcaaacttga cttcagcacg cgtcttgtag
      421 gtcccgtcat ctttgaaaga tatagtgcgt tcctgtacat aaccttcggg catggcactc
      481 ttgaaaaagt catgccgttt catgtgatcc ggataacggg aaaagcattg aacaccatag
      541 gtcagagtag tgacaagtgt tggccatgga acaggtagtt ttccagtagt gcaaataaat
      601 ttaagggtga gttttccgtt tgtagcatca ccttcaccct ctccacggac agaaaatttg
      661 tgcccattaa catcaccatc taattcaaca agaattggga caactccagt gaaaagttct
      721 tctcctttgg aaccaccact accggaccca ccggatccgt ctgcatatgg actccagtcg
      781 gtactccatc ccacaaaaga attcttgttc tggtctttca gccaggtaaa taagggctca
      841 aagtagttga gcagtggcct tacattcatg ttctttgctc ctacaacatt ttccaatgct
      901 a
//
