LOCUS       sequence-288179-         524 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET vector for IPTG-inducible, T7-based expression of RTX bearing a 
            C-terminal 6xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Bhadra S, Maranhao AC, Paik I, Ellington AD
  TITLE     A one-enzyme RT-qPCR assay for SARS-CoV-2, and procedures for 
            reagent production
  JOURNAL   BioProtocol 2021
REFERENCE   2  (bases 1 to 524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BioProtocol
            2021"
COMMENT     Sequence Label: pET_RTX-6xHis
FEATURES             Location/Qualifiers
     source          1..524
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctagaatntt ttgtttnntt taagaaggag atatacatat gattcttgac acngnttnnn
       61 tcacggaaga cggcaagccg gttatccgta ttttcaagaa agaaaacggc gaattcaaga
      121 ttgaatacga tcggacattt gaaccgtacc tgtacgctct cctcaaggat gatagcgcaa
      181 tcgaagaagt gaaaaaaatc accgcagagc ggcatggcac agtggtaaca gttaagcggg
      241 tcgagaaagt gcagaagaag ttcttaggcc ggccagtcga agtatggaaa ttatacttca
      301 cacatccaca ggacgttccg gcgatcatgg ataagattcg ggagcatccg gcggtaatcg
      361 atatctatga atacgatatt ccgttcgcta ttcgctacct tattgacaaa ggtttagttc
      421 caatggaggg tgatgaggaa cttaaactgt tagcattcga tatcgaaaca ctttatcacn
      481 aaggtgaaga gtttgccgaa ggtccnattt taatgatctc gtac
//
