LOCUS       sequence-288180-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET vector for IPTG-inducible, T7-based expression of RTX bearing a 
            C-terminal 6xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Bhadra S, Maranhao AC, Paik I, Ellington AD
  TITLE     A one-enzyme RT-qPCR assay for SARS-CoV-2, and procedures for 
            reagent production
  JOURNAL   BioProtocol 2021
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BioProtocol
            2021"
COMMENT     Sequence Label: pET_RTX-6xHis
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..56)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             457..465
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggctttgtta gcagccggat caatgcggcc gcttatcagt gatggtgatg gtgatggctg
       61 gatccctcga gggtaccttt cggcttcaga cgggcactca aaccgacttg acgagttttc
      121 tggtaccgca aatcctcctt gcgatagccg aacgcacgta agatgcgctc caccgccggc
      181 agaacctgtt tttcaatgta atattccgca tcatatttat gcttcgtcgg atcaaattca
      241 tcaaatggaa tcgcgcgatc aacaatccgg cctgagcctt tgaggacgat gtaactaata
      301 accgtgcctg gccgaatctt gacgccccga gccgcaagcc gctttgctac ggcgacgtgt
      361 ggaccagtgg ccttatagtc tttaaggtcg cgagtgattt gtttatgaat aacaagtttc
      421 tctggtggga cctcatactt gctaagcttc tctgtaacct ccttaacgat gcgtacggct
      481 ttttcgacgt caccatcctt caggagggcc tccaacacac gagcttgggt ctcctttgcg
      541 atctcgctcc aatcgcgacg aacaatttcc aagccacgag ttgtgatctt gccttcctcg
      601 tcaattaccg cgtatttctt ctttgtcacg aacaggccac gcttataaaa accttcgtac
      661 tctaattcca gggcgcctgg caacttcgca ttaatatact tcaagaactc cattgccttt
      721 ttctttacag tttctgcgtc cgcacctgga atcgttgcaa agaaaccatc cgtgtcacta
      781 tagatgacct tgaaaccgta tttctcctca atttccttaa tcgtcatggt caggtactcc
      841 cgaccccaag caatgactga ttctgcacat tctttgcaat accaccgggc gcgtg
//
