LOCUS       sequence-288235-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for sACE2-sfGFP high affinity variant 
            3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert 
            AS, Procko E
  TITLE     Engineering human ACE2 to optimize binding to the spike protein of 
            SARS coronavirus 2.
  JOURNAL   Science. 2020 Sep 4;369(6508):1261-1265. doi: 
            10.1126/science.abc0870. Epub 2020 Aug 4.
  PUBMED    32753553
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc0870"; journalName: "Science"; date: "2020-09-4-
            4"; volume: "369"; issue: "6508"; pages: "1261-1265"
COMMENT     Sequence Label: pcDNA3-sACE2v3-sfGFP
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..728)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             complement(18..728)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(58..78)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     673..701
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctagactcga gttatcattt gtagagctca tccatgccat gtgtaatccc agcagcagtt
       61 acaaactcaa gaaggaccat gtggtcacgc ttttcgttgg gatctttcga aaggacagat
      121 tgtgtcgaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat tggagtattt
      181 tgttgataat ggtctgctag ttgaacggaa ccatcttcaa cgttgtggcg aattttgaag
      241 ttagctttga ttccattctt ttgtttgtct gccgtgatgt atacattgtg tgagttaaag
      301 ttgtactcca gtttgtgtcc gagaatgttt ccatcttctt taaaatcaat accttttaac
      361 tcgatacgat taacaagggt atcaccttca aacttgactt cagcacgcgt cttgtaggtc
      421 ccgtcatctt tgaaagatat agtgcgttcc tgtacataac cttcgggcat ggcactcttg
      481 aaaaagtcat gccgtttcat gtgatccgga taacgggaaa agcattgaac accataggtc
      541 agagtagtga caagtgttgg ccatggaaca ggtagttttc cagtagtgca aataaattta
      601 agggtgagtt ttccgtttgt agcatcacct tcaccctctc cacggacaga aaatttgtgc
      661 ccattaacat caccatctaa ttcaacaaga attgggacaa ctccagtgaa aagttcttct
      721 cctttggaac caccactacc ggacccaccg gatccgtctg catatggact ccagtcggta
      781 ctccatccca caaaagaatt cttgttctgg tctttcagcc aggtaaataa
//
