LOCUS       sequence-288252-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high 
            affinity 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert 
            AS, Procko E
  TITLE     Engineering human ACE2 to optimize binding to the spike protein of 
            SARS coronavirus 2.
  JOURNAL   Science. 2020 Sep 4;369(6508):1261-1265. doi: 
            10.1126/science.abc0870. Epub 2020 Aug 4.
  PUBMED    32753553
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc0870"; journalName: "Science"; date: "2020-09-4-
            4"; volume: "369"; issue: "6508"; pages: "1261-1265"
COMMENT     Sequence Label: pcDNA3-sACE2v2-Fc(IgG1)
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    261..382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(298..317)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     352..371
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(431..447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(431..447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(444..466)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    455..471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(479..485,486..503,504..509))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    524..545
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(662..679)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
       61 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
      121 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
      181 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
      241 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
      301 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
      361 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
      421 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      481 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      541 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      601 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      661 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      721 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      781 caaa
//
