LOCUS       sequence-288254-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike)
            with 8his tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert 
            AS, Procko E
  TITLE     Engineering human ACE2 to optimize binding to the spike protein of 
            SARS coronavirus 2.
  JOURNAL   Science. 2020 Sep 4;369(6508):1261-1265. doi: 
            10.1126/science.abc0870. Epub 2020 Aug 4.
  PUBMED    32753553
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc0870"; journalName: "Science"; date: "2020-09-4-
            4"; volume: "369"; issue: "6508"; pages: "1261-1265"
COMMENT     Sequence Label: pcDNA3-SARS-CoV-2-S-RBD-8his
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..41)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(714..733)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(715..733)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(778..877)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(807..827)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctagactcga gttatcaatg atgatggtgg tggtgatggt gaccggatcc ctttttgggc
       61 ccacaaactg tagccggggc gtggaggagc tcaaaggaca atactaccac tctataaggt
      121 tgatacccca ctccgtttgt tggttgaaag ccataagatt gaagaggaaa gtagcaatta
      181 aatccctcta ctccgttaca tggcgtactg cctgcttggt aaatttcggt tgaaatatcc
      241 cgctcaaaag gcttcaaatt gctctttcga aacaggcgat acaagtaatt ataattacca
      301 cctactttgg agtcgaggtt gtttgagttc catgcgatta cgcaccccgt aaaatcatcg
      361 ggcaacttat agttgtaatc cgctatcttc ccagtctgac ctggtgctat ctgtctcact
      421 tcgtcccccc gtataacgaa agagtcagca tacacgttcg taaaacagag atcattcagc
      481 ttcgttggag aaacgccgta acacttaaag gttgaaaagg acgcactgtt atagagaacg
      541 gagtaatcag ctacgcaatt ggagattctc tttcggttcc aagcatatac actagcgaac
      601 cttgtcgcgt taaaaacctc accgaacgga caaagattag tggcgaacac caggcagaag
      661 atgtagctca gggcgatgat ggtcttcatg gtggcgctag ccagcttggg tctccctata
      721 gtgagtcgta ttaatttcga taagccagta agcagtgggt tctctagtta gccagagagc
      781 tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
      841 gttgttacga cattttggaa agtcccgttg attttggtgc c
//
