LOCUS       sequence-288255-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike)
            with 8his tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert 
            AS, Procko E
  TITLE     Engineering human ACE2 to optimize binding to the spike protein of 
            SARS coronavirus 2.
  JOURNAL   Science. 2020 Sep 4;369(6508):1261-1265. doi: 
            10.1126/science.abc0870. Epub 2020 Aug 4.
  PUBMED    32753553
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc0870"; journalName: "Science"; date: "2020-09-4-
            4"; volume: "369"; issue: "6508"; pages: "1261-1265"
COMMENT     Sequence Label: pcDNA3-SARS-CoV-2-S-RBD-8his
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    248..369
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(285..304)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     339..358
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(418..434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(418..434)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(431..453)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    442..458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(466..472,473..490,491..496))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    511..532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(649..666)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
       61 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
      121 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
      181 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
      241 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
      301 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
      361 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
      421 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      481 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      541 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      601 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      661 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      721 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      781 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      841 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      901 aagatacca
//
