LOCUS       sequence-288260-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for sACE2-sfGFP high affinity variant 
            5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert 
            AS, Procko E
  TITLE     Engineering human ACE2 to optimize binding to the spike protein of 
            SARS coronavirus 2.
  JOURNAL   Science. 2020 Sep 4;369(6508):1261-1265. doi: 
            10.1126/science.abc0870. Epub 2020 Aug 4.
  PUBMED    32753553
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc0870"; journalName: "Science"; date: "2020-09-4-
            4"; volume: "369"; issue: "6508"; pages: "1261-1265"
COMMENT     Sequence Label: pcDNA3-sACE2v5-sfGFP
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctcttcctg gctccttctc agccttgttg ctgtaactgc tgctcagtcc accattgagg
       61 aacaggtcaa gacatttttg gacaagttta accacgaagc cgaagacctg ttctatcaaa
      121 gttcacttgc ttcttggaat tataacacca atattactga agagaatgtc caaaacatga
      181 ataatgctgg ggacaaatgg tctgcctttt taaaggaaca gtccacactt gcccaaatgt
      241 atccactaca agaaattcag aatctccaag tcaagcttca gctgcaggct cttcagcaaa
      301 atgggtcttc agtgctctca gaagacaaga gcaaacggtt gaacacaatt ctaaatacaa
      361 tgagcaccat ctacagtact ggaaaagttt gtaacccaga taatccacaa gaatgcttat
      421 tacttgaacc aggtttgaat gaaataatgg caaacagttt agactacaat gagaggctct
      481 gggcttggga aagctggaga tctgaggtcg gcaagcagct gaggccatta tatgaagagt
      541 atgtggtctt gaaaaatgag atggcaagag caaatcatta tgaggactat ggggattatt
      601 ggagaggaga ctatgaagta aatggggtag atggctatga ctacagccgc ggccagttga
      661 ttgaagatgt ggaacatacc tttgaagaga ttaaaccatt atatgaacat cttcatgcct
      721 atgtgagggc aaagttgatg aatgcctatc cttcctatat cagtccaatt ggatgcctcc
      781 ctgctcattt gcttggtgat atgtgg
//
