LOCUS       sequence-288264-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for J chain of polymeric antibody 
            classes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert 
            AS, Procko E
  TITLE     Engineering human ACE2 to optimize binding to the spike protein of 
            SARS coronavirus 2.
  JOURNAL   Science. 2020 Sep 4;369(6508):1261-1265. doi: 
            10.1126/science.abc0870. Epub 2020 Aug 4.
  PUBMED    32753553
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc0870"; journalName: "Science"; date: "2020-09-4-
            4"; volume: "369"; issue: "6508"; pages: "1261-1265"
COMMENT     Sequence Label: pcDNA3-J chain
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(519..538)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(520..538)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(583..786)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(612..632)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctagactcga gtcattagtc tgggtagcac gcatccggcg tcagagccgt ctccaccatt
       61 tttgtttctc ctccgtatac caacggaacg accgctgtat agcatttatt ccggtcataa
      121 gtgtaacaag tctcggttgc agaatcttca tcgcaaatat tggattgagt agcagtcacg
      181 atttgattgt ccagctctac ctctgtcggg tcacattttt tgcagaggtc ggaaagatgg
      241 tatacgaacc tcgtcctaag ggggctagtc gggtcgctta tgttttccct attgttaagg
      301 ggaacaatga tcctaatatt ccgctcgaca atgtcttcat tgggatcttc actggatctt
      361 attattcttg acgtaattct ggcgcactta cacttattgt caaccaggac tattctctcg
      421 tcttcctgag cctttacatg gacggctttg atgaagaccg cgagtacacc ccaaaagaga
      481 aggtggttct tcatggtggc gctagccagc ttgggtctcc ctatagtgag tcgtattaat
      541 ttcgataagc cagtaagcag tgggttctct agttagccag agagctctgc ttatatagac
      601 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
      661 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
      721 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
      781 catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagt
//
