LOCUS       sequence-288265-         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for J chain of polymeric antibody 
            classes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert 
            AS, Procko E
  TITLE     Engineering human ACE2 to optimize binding to the spike protein of 
            SARS coronavirus 2.
  JOURNAL   Science. 2020 Sep 4;369(6508):1261-1265. doi: 
            10.1126/science.abc0870. Epub 2020 Aug 4.
  PUBMED    32753553
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc0870"; journalName: "Science"; date: "2020-09-4-
            4"; volume: "369"; issue: "6508"; pages: "1261-1265"
COMMENT     Sequence Label: pcDNA3-J chain
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(514..531)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    520..631
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 accaccttct cttttggggt gtactcgcgg tcttcatcaa agccgtccat gtaaaggctc
       61 aggaagacga gagaatagtc ctggttgaca ataagtgtaa gtgcgccaga attacgtcaa
      121 gaataataag atccagtgaa gatcccaatg aagacattgt cgagcggaat attaggatca
      181 ttgttcccct taacaatagg gaaaacataa gcgacccgac tagccccctt aggacgaggt
      241 tcgtatacca tctttccgac ctctgcaaaa aatgtgaccc gacagaggta gagctggaca
      301 atcaaatcgt gactgctact caatccaata tttgcgatga agattctgca accgagactt
      361 gttacactta tgaccggaat aaatgctata cagcggtcgt tccgttggta tacggaggag
      421 aaacaaaaat ggtggagacg gctctgacgc cggatgcgtg ctacccagac taatgactcg
      481 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
      541 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
      601 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
      661 tggggg
//
