LOCUS       sequence-288275-        6975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter plasmid for DE OCT4 enhancer activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D,
            Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, 
            Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad
            S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, 
            Novershtern N, Hanna JH
  TITLE     Derivation of novel human ground state naive pluripotent stem cells.
  JOURNAL   Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 
            2013 Oct 30.
  PUBMED    24172903
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12745"; journalName: "Nature"; date: "2013-12-12- 
            12"; volume: "504"; issue: "7479"; pages: "282-6"
COMMENT     Sequence Label: pGL3-human OCT4 DE-SV40-Luc
FEATURES             Location/Qualifiers
     source          1..6975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    587..635
                     /note="synthetic polyadenylation signal"
     misc_feature    649..740
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     689..708
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2760..2956
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2807..2942
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      2986..2995
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2992..4644
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3058..3076)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    4685..4806
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4696..4715)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4750..4769
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5054..5071)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5225..5813)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5305..5324)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5984..6775,6776..6844))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6607..6626
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6845..6949)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
      541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt
      601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
      661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
      721 aggtgccaga acatttctct atcgataggt acccattgag tccaaatcct ctttactagg
      781 tgaggaaact gaggcacagg agggggaggt gccattagcc agggagtcag agccaggact
      841 tagaacctgg tccatgcctc ctagtttgtt cctgaccaat cctcccagct cacccactcc
      901 ttttgctctc acctctgact ccctacaggc tacagctaga gagttgactc ctctatttga
      961 ggtaagagtg tctggatttg ggataaggtg acctcagttt gcaggtctca tgggggagat
     1021 cagagggtcc tttgttgaga agaggggact tggtccacag tgagcaaagg aatttgtcta
     1081 aattgctatt aagggagatc atctcccaag gtgggccaag cctgaaccct cagccctcca
     1141 tttcttccct ttcttttgcc ttcagattga caggcctcgg aagtcaaaat aagtggtttc
     1201 ctagaccggg tcgagagcaa gtctctattg gtcccaactg agttttttca gctggttttt
     1261 caaccaaaca gcacctcatc tcccagtgag gggaagggaa ggctgggctg acagcagcaa
     1321 ggctgctcat ctcacctctc cccacccagc catgccagcc gccacacctg gtggggagag
     1381 gtgggcctca cctgggtccc ctggcagtgc tctgtgaagg gtcttgacat tgcactgtaa
     1441 taataaaggt gtgtgtgaag tatcttttta tggtgacttt ctaaaaccca gggaatcatg
     1501 ggaccagttc tgatgactca gcctggcttc cagtctcctc caggcccaac ggtggccccc
     1561 agcactggtt ggggcctggg agagctggcc ttggctgaag tgaagccacc tacccttcag
     1621 gcataacagg acagtgagaa ggaaggaaag cctgcctcaa cctcccatca gccctgagca
     1681 ccccagaagg gggccggcta ggagtctagg catgcaggag gctgacccct gactgggctc
     1741 atatccagcc acaaggcagc cagggaccca ggcacccacc ccttgtctgc gtccctctcg
     1801 ggaatgggcc tcttgcccag gccagaaata caccacctac agtacaaatt ataatctaaa
     1861 aacaagaggg tggtgttgag tggggaaatt ggggaaggtg ttttaggagc cactaggaaa
     1921 atgggcagca gggactctct ggactggctt gggaagagcg cttttgggga acctggagga
     1981 tggcaagctg agaaacactg gtgcggagat tccagccaaa tcccaggcct gcccctcccc
     2041 ctcctctgag aggccgtctt cttggcagac agcagagaga tgcatgacaa aggtgccgtg
     2101 atggttctgt cctggggatt gagatggctg gggaggggcc tcctcctgtt ccgaagcatg
     2161 ttcctcccac ccccaccagg ccccataatc tacctgcctt ttgggcagtt aaaggccgag
     2221 aagtgaacac agctccaacc ccactgcctt gtagaccttc cggcagacct gtggcaggta
     2281 ttgaaatgca cgcatacaat taggctcaaa aagtctacac agacaggaga tgggcacacg
     2341 aacagaggca acataagagt gggggaaaag tctcaaaaga ctcacggatg ccaccaagat
     2401 gaagacagct ggccacggga cacccatccc cttagaaggc agacagagcc actgacccca
     2461 gcagacaagc ccaggcaggg ctgagcctgg agcctgcaat gagaagcctt acttaagtcg
     2521 acagaggtca gcgtgcccag tccagacctg gccttctggc cttcgaagct gtggggagcc
     2581 ctggcccaga gccccctctg gagcccccag acttacccca ggccctccac tgagatcaag
     2641 ttttgggagc agacagacaa acatcatccc tcacagacag gcattccgtt ggctattctc
     2701 ttgcaaacag aatcaagcac tagaccagca gcatgagcct cagctcgaga tctgcgatct
     2761 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     2821 tccgcccagt tccgcccatt ctccgcccca tcgctgacta atttttttta tttatgcaga
     2881 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     2941 cctaggcttt tgcaaaaagc ttggcattcc ggtactgttg gtaaagccac catggaagac
     3001 gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga
     3061 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca
     3121 gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg
     3181 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa
     3241 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg
     3301 cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct
     3361 accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc
     3421 ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg
     3481 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca
     3541 gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt
     3601 ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga
     3661 gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc
     3721 catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc
     3781 ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa
     3841 agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa
     3901 tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc
     3961 ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact
     4021 gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt
     4081 aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc
     4141 gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac
     4201 aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata
     4261 gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag
     4321 tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac
     4381 atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc
     4441 gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc
     4501 agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg
     4561 aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag
     4621 aagggcggaa agatcgccgt gtaattctag agtcggggcg gccggccgct tcgagcagac
     4681 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     4741 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     4801 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     4861 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc
     4921 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     4981 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     5041 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5101 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5161 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5221 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5281 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5341 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5401 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5461 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5521 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5581 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5641 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5701 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5761 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5821 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5881 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5941 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6001 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6061 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6121 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6181 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6241 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6301 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6361 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6421 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6481 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6541 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6601 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6661 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6721 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6781 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6841 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     6901 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     6961 gaaaagtgcc acctg
//
