LOCUS       sequence-288296-        8068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmAUB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8068)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmAUB_A
FEATURES             Location/Qualifiers
     source          1..8068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(2865..2882)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5353..5394
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5404..5421
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5444..5461)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5494..5628
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5518..5537)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5572..5591
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6250..6838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6330..6349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7009..7800,7801..7869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7632..7651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7870..7974)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8042..8060
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agctttatga atttaccacc aaaccctgta attgctcgtg gacgtggtcg
     2761 aggaagaaag cccaataatg tagaggccaa tcgcggcttc gctccttcgc tgggtcagaa
     2821 gtcggaccct tcccactcgg aaggaaatca agcatccgga ggcaatggtg gtggtggtga
     2881 tgctcaagtg ggtccctcga tagagaaatc cagtttgagc gcggtgcaaa tgcacaagtc
     2941 cgagggcgat ccgcgtggct ccgtgcgcgg ccgtaggcta ataacggatt tggtgtattc
     3001 acgtcctcca gggatgacca gcaagaaagg agtggttggc actcatatta ccgtgcaggc
     3061 aaactatttt aaggtattaa agcgtccaaa ctggaccatc taccagtacc gcgtcgattt
     3121 tacgcctgat gtggaggcta cacgactacg acggtctttc ttgtatgaac ataaagggat
     3181 cctgggcggc tacatctttg acggaaccaa tatgttttgc atcaatcagt tcaaagctgt
     3241 tcaagatagc ccctatgttt tggaactggt tacgaagagt cgtgctggcg aaaacattga
     3301 aattaagatc aaggctgttg gatctgtgca atctacggat gccgagcagt ttcaggtcct
     3361 taatctcata ctgcgcaggg ccatggaggg cttagacttg aagctggtct cgcgctacta
     3421 ctacgatcct caagctaaga ttaatttgga gaatttccgc atgcaattat ggcctggcta
     3481 tcagacttcg attcgccagc acgaaaatga tatattactt tgttccgaga tatgccacaa
     3541 ggttatgcga actgagacct tgtacaatat tttgtccgat gctattcgcg acagtgacga
     3601 ttatcaaagt acatttaagc gtgcagtaat gggtatggta atactaacgg attataataa
     3661 caaaacctat cgcattgacg atgtcgactt tcaatcgacg ccattgtgca aatttaaaac
     3721 caatgacggt gagatttcgt acgtggatta ctataagaag cgatacaaca taatcattcg
     3781 cgacttgaag caacctctgg tcatgtctcg tccgacagac aaaaatattc gtggtggaaa
     3841 tgaccaggcc ataatgatca ttcccgagct ggcacgggct acgggaatga cggacgctat
     3901 gcgcgctgac tttaggacgt tgagggccat gagtgaacat accaggctga atccagatcg
     3961 tcgcatcgaa cgccttcgca tgttcaacaa gcgcttaaag tcgtgtaaac agagtgtaga
     4021 gacccttaag tcctggaaca tcgagctgga ctccgccttg gtggagattc cagcacgcgt
     4081 gttgccaccg gaaaaaatat tattcggcaa ccaaaaaata ttcgtatgcg acgctcgcgc
     4141 cgattggacc aatgagtttc gcacctgttc gatgtttaaa aacgtgcata tcaataggtg
     4201 gtatgtgatc acaccgagtc gaaatctgcg cgagacccag gaatttgtgc agatgtgcat
     4261 ccgcacagcc agcagcatga agatgaacat ctgcaatcca atatatgagg aaattccgga
     4321 tgaccgcaac ggcacttact cccaagcgat cgacaacgcc gcagctaacg atccacagat
     4381 cgtgatggtt gtcatgagat ctccaaatga agagaaatat agctgcatta aaaaacgcac
     4441 gtgcgtggac agaccggtgc cgtcgcaggt ggtgacacta aaagttatcg cgcctcgaca
     4501 gcaaaaacca actgggctga tgtcgatcgc cacaaaggtg gttattcaaa tgaacgccaa
     4561 attgatggga gctccctggc aggtagtgat ccccctccac ggtctgatga ctgttggttt
     4621 cgatgtctgc cattcaccga agaataagaa caagtcatat ggggctttcg ttgcaaccat
     4681 ggaccagaag gagtctttcc gctacttctc caccgtaaac gaacacatta agggccaaga
     4741 gttgtccgag cagatgtcgg tgaacatggc gtgcgccctg aggtcgtatc aggagcaaca
     4801 ccgttctttg ccagagcgca ttctcttctt tcgcgacggt gttggcgatg gtcagctcta
     4861 ccaggtggta aacagcgagg tgaacaccct aaaggacagg ctcgacgaaa tttacaaatc
     4921 agctggcaaa caggagggct gtcgcatgac atttataatt gtatccaagc gcattaattc
     4981 tcgctacttt actgggcatc gcaacccagt tccgggcact gtagtcgatg acgttattac
     5041 cttgccagag cgctacgact tcttcctagt gtcccaggct gttcggatag gaactgtgtc
     5101 gcctaccagc tacaatgtta tttctgacaa catgggacta aacgccgata agctgcagat
     5161 gctctcctat aagatgaccc atatgtacta caattacagc ggaaccatac gagtccccgc
     5221 tgtctgccat tacgcccaca aattggcttt cctcgtagcc gaatccatta atcgtgcgcc
     5281 ttcagcagga ctgcagaatc aattgtactt tttgtaagcg gccgctcgag tctagagggc
     5341 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
     5401 ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct
     5461 aaggcctgag ctcgctgatc agcctcgatc gaggatccag acatgataag atacattgat
     5521 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     5581 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     5641 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     5701 aacctctaca aatgtggtat ggctgattat gatcagtcga ccgatgccct tgagagcctt
     5761 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     5821 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg
     5881 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat
     5941 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa
     6001 gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
     6061 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
     6121 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
     6181 aggatcgctc gcggctctta ccagccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6241 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6301 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6361 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6421 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
     6481 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6541 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6601 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6661 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     6721 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6781 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6841 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6901 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6961 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7021 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7081 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7141 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7201 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7261 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7321 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7381 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7441 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7501 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7561 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7621 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7681 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7741 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7801 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7861 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7921 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7981 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     8041 aaataggcgt atcacgaggc cctttcgt
//
