LOCUS       sequence-288324-       10326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmXRN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10326)
  AUTHORS   Haas G, Braun JE, Igreja C, Tritschler F, Nishihara T, Izaurralde E
  TITLE     HPat provides a link between deadenylation and decapping in metazoa.
  JOURNAL   J Cell Biol. 2010 Apr 19. 189(2):289-302.
  PUBMED    20404111
REFERENCE   2  (bases 1 to 10326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2010 Apr 19. 189(2):289-302."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmXRN1_A
FEATURES             Location/Qualifiers
     source          1..10326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7611..7652
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7662..7679
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7702..7719)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7752..7886
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7776..7795)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7830..7849
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8508..9096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8588..8607)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9267..10058,10059..10127))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9890..9909
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10128..10232)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10300..10318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat ccagcacagt ggcggccgca tgggcgttcc
     2761 caagttcttt cgctacatca gcgagcgata tccgtgcctc agcgaattgg cgcgggagca
     2821 ctgcataccc gagttcgaca atctgtactt ggacatgaac ggcattgtcc acaactgctc
     2881 gcatccggac gacaacaaca tacactttca cctggaggag gagcagatat tccaggaaat
     2941 cttcaactac gtagacaagc tgttctacct gatcaagccg cagcgcctct tcttcctttc
     3001 agttgacggg gtggctccac gggccaagat gaaccagcag cgcagccggc gattccggac
     3061 ggcgcgcgag gcggagcagc aggaggccaa ggcggcccag cgaggcgagt tgcgagagca
     3121 cgagcgcttc gacagcaact gcattacgcc gggcaccgaa ttcatggtgc gtctgcagga
     3181 gggactgcgc gcctttctca aaacgaagat ttcgacggat ccgctgtggc agcgttgcac
     3241 cgtaatccta agtggccagg aggcgcccgg cgagggcgag cacaagatca tggactacat
     3301 tcggtacatg aagacgcagc cggactatga tcccaataca cgacactgcc tttacggcct
     3361 ggacgccgat ctgatcattc tgggcctctg cacccacgag ctgcacttcg tggtgctgcg
     3421 cgaggaggtc aagttcgggc gcaacgtcaa gcgcacctcg gtggaggaga cgcgcttctt
     3481 cctgctgcac ctgggtctgc tgcgcgagta cctcgagctg gagttcgacg ccctgagaac
     3541 agacgagcac aaattggaca ttgcgcagct tatcgacgac tgggtgctga tgggcttttt
     3601 ggtcggcaac gacttcattc cccacctgcc gtgcctgcac atttcatcaa atgcactgcc
     3661 gctgctctac cgcacctaca tcggcattta cccgaccctc ggcggcaaca tcaacgagaa
     3721 cggcaagctc aacctgcgcc gcttgcagat tttcatatcg gccctcaccg aggtggagct
     3781 ggatcacttc aaggagcacg ccgacgactt gaagtacatg aacaacaaat cagaggcgtt
     3841 cgacatggac gtcggcgaga tcaccgagtc tcaaaacctc gactccgatc tcggagccct
     3901 catcaacaag agcatgctgc tctacgatga cgacagcgag gaggactgct ccgacgagaa
     3961 cgcggttctt ctgaaggagt tccagaacta caagcgaaac ttctaccgca acaagttcaa
     4021 gcgtgatccg aatgatgaac tgatcgagga actgtgccac cactacgtca atgcgctgca
     4081 gtgggtgctc gactactact accgtggcgt gcagtcctgg gactggtact atcccttcca
     4141 ctacactcca ttcatcagcg acctcaagaa catcgagcag gtggagatcg ccttccacat
     4201 gggcacgccc tttctgccat tccagcagct tctggcggta ctgccggcgg ccagtgcaaa
     4261 gcttctccct gtcgcctacc acgacctcat gctgctgccg accagcccgc tggccgagtt
     4321 ctatccactg gagttcgaga gcgacctcaa tggcaagaaa cacgattggg aggcggttgt
     4381 gctgataccc ttcatcgatg agggtcggct gctggccgct atgctgccat gcgaggctca
     4441 gctgtcgctg gaggagcgcg aacgcaaccg acacggtccc atgtatgtgt acaaatacag
     4501 tacggtggcg cagggtccca tgccagcgta tccaccgctg agggctcttc ccgtgctcta
     4561 ctgcacggag gtggccaagt ggtcgcacga aatcgcagtc aatctgccgt acagcgtgtg
     4621 catcgagcta ccaaatgcgg cgagaaccgt tttcttcccg ggatttccca ccatgcagca
     4681 tctgcccttc gatttcgagc tgcgcaacga ccgcgtcaag gtgttcgaac aggtcagtcg
     4741 caaccagaac attgtgctaa aaccacgcaa gcgccagctg gaggacacac tgacggcggt
     4801 ggcctcccag tacctcggga aggtgatcca cgtcggctgg ccgcatctcg tcaaggcgat
     4861 agtggtgcgc gtggccacgc gagatcagcg cgtcgacagc gagggaatca cgctaaacga
     4921 ttcgcggcgt ttcgactcgg aatgcaaggc actgcaggaa cactttatca atcgtatggg
     4981 aatacagttt gccaactatg atgtgctcgt ctatgtgcgc acctttgccg gcaattcgac
     5041 ggagttccgc gacaaggggg cattgatggt gcgcgactcg tggagcagct ccgtcaccgg
     5101 ctatccagcc cagggcgtgg tggccgatct gactgtgtgg gaaagaatgc gcaagaattt
     5161 cctcaacgtg gagcactact tccccgttgg cagcacgatc ttcttgatca cggatccgta
     5221 ttacggcagc gagggaactg tgcaggaccc acggctggcc tacaccaacg gccggattca
     5281 agtcagcatc atggtgcgac cggaaccgaa agtaaatgcg gcacgtcagc tgcaggaaga
     5341 gcgggaccgg gactatctga gcaccttcca ggtctgcaac ttgctccgaa tcagtggccg
     5401 caccctggga cgcctaagtg gcaccgtgtg ggtggtgctc ggtccgcggc gtcagaagat
     5461 ggaaaacgtg accaagcaca acattggcct gcagctcaag tatccgcggc agaacgagga
     5521 gcgtgccgga tactgcttcc gcacgaacaa ccagtggtac tactccagcc tggccgtgga
     5581 tctgatgcgt aactattgcc agcgttaccc agacgttatc gacttctttg gcgactccaa
     5641 cgatcgcgcc gagttcgtat tcgaacagga cgtgttcccc aatgcggttg gccatcgccg
     5701 cgtcgaggaa ctggccaact gggtgcgcca gcagccgcac atgaaagtgg agcgcatctc
     5761 ttgcggctcc aagacggtct gccgcgagac catcgaactg ctgatagccg ccgtcgacga
     5821 cctgcgctca ctgcccgtca agcacgttaa actgcaggtg aagccgcact tgctcatcaa
     5881 gcccaatgtc acactgccag atgtctaccg atcaaagcgc ccggtgcgtc tcttcgatcg
     5941 cgtcgtaatc gtacgcacca tttacatggt gcccgtgggc actaagggca ccgtgattgg
     6001 cattcatccc gttacggacc ccaacccggt ccgcctggag tgcgtccatg ctgtcgacac
     6061 gttctgcaag gtgctgttcg acagccccgt tccgaactgc aacaacatcc atggcatcgc
     6121 cgaagaccga gtttacaagg tgcccgaaat tgccctggtg atcatcaaga cggatgagga
     6181 aggaaagaag cagaacgact gcgaactgcc ggtgagggat cctcagccga accaggcaca
     6241 ggacgagcca gtccgcgcga ccagcagtcg ctatgtgacc gccgcagggt ccacctcggt
     6301 cccgatcacg atgaagaccc aaattagcga cgaattcgtc aagacgcgta gcgatccgat
     6361 agcccgtacg gattcgtaca agccatcgtc ggagccaaaa ccggtgccgg tgccggagca
     6421 gattaccaac tggcgggaac gcgtcagtac gccaacaaac aaaccgcagc ctgcgccaaa
     6481 taattggcgc ataaatcggt cttcatcacg tcaacagggc ggtagtattt ttgttgctcc
     6541 gcccacaaaa actcctgatg cagctgcaag cactgcttcc actgcattca ccgcagcctc
     6601 gtccgcaacg ctgactcctc tggatcagac cttggcgctc atgtcggttt tgggcgtggg
     6661 tgaggaccag agctcgccgc cactccagga ggcggtgcag cagcaacgtc cgccactact
     6721 tcaacagcaa agagctccgt ttcccggaca aatgcccaat ctacccaagc caccgctctt
     6781 ctggcagcaa gaggcgcaga agcaggaggc gctgcagcag gaggcgcagc agcaggaggc
     6841 gcagaagaag cagcagcagg cgcatgcgca aatggagcct gaacgtatca atagccagca
     6901 tttctacaga agcggccaga caggagcggc gttgaatcag ccgccattgg gtgctccatc
     6961 aaaaaggcag tggcatgagt gggtgcaccc gcgcatgcag cacgccaacg cattccacgc
     7021 tggcgtcaac aatggatatc agatgcgtcc caaaaagaat atagctgcgc agagcacctt
     7081 taacaacaac gtgcacatgc acctccagca accatattac ccgaatcagc agcagcagca
     7141 gcagcagcag cagccactcc agctgacgga gatcaacaat gcgccaccgc gatactcaac
     7201 gatccaagat tttgtgccca tacaggcgta ccgtcccaag aaattaaatc gcgtccaacc
     7261 agccggtcgt caggacgtcg atgccaccaa gaatcctagt cgttcgcctg tgttgcagca
     7321 accgacgaac gagactatag acacaaaggc cagttctagt cttccggtac aatcagcagg
     7381 cgaacaggtg attggactga tgcaaacgct tgaaattaag ccggctgcgt cgcagtccga
     7441 atctgatggg gtctcaacag gcagtgcgaa cgcgcccaca gcaacgacat catcccaggc
     7501 ggtcaaccga aggaagcatc gagtgcctcg catcggagcc aagttcgact tggaatacat
     7561 actgcccgac tccccgcatc caacttaatc tagagggccc gcggttcgaa ggtaagccta
     7621 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt
     7681 gagtttaaac ccgctgatca gcctcgactg tgccttctaa ggcctgagct cgctgatcag
     7741 cctcgatcga ggatccagac atgataagat acattgatga gtttggacaa accacaacta
     7801 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     7861 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     7921 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     7981 ctgattatga tcagtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     8041 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     8101 gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
     8161 gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     8221 ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     8281 gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc
     8341 cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg
     8401 tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc
     8461 agccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8521 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8581 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8641 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8701 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8761 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8821 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8881 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8941 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9001 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9061 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9121 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9181 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9241 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9301 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9361 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9421 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9481 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9541 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     9601 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9661 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9721 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9781 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9841 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     9901 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9961 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10021 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10081 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10141 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10201 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    10261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    10321 tttcgt
//
