LOCUS       sequence-288338-        8390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmHPat.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Tritschler F, Eulalio A, Helms S, Schmidt S, Coles M, Weichenrieder 
            O, Izaurralde E, Truffault V
  TITLE     Similar modes of interaction enable Trailer Hitch and EDC3 to 
            associate with DCP1 and Me31B in distinct protein complexes.
  JOURNAL   Mol Cell Biol. 2008 Nov . 28(21):6695-708.
  PUBMED    18765641
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2008 Nov . 28(21):6695-708."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmHPat_A
FEATURES             Location/Qualifiers
     source          1..8390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5675..5716
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5726..5743
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5766..5783)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5816..5950
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5840..5859)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5894..5913
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6572..7160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6652..6671)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7331..8122,8123..8191))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7954..7973
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8192..8296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8364..8382
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catggatgac tcgtttttcg gctttgacac
     2761 gaatctaccg gacgaagatg gtggcgatgg acgcatcgtc gaggcggaat acgatgccct
     2821 caatgacgaa acctttggat ccgctataaa tggcgactgg gaggaagccc atgagacgat
     2881 ggtacgcctg ggcgggaatg gcgaaagggt gcggaagcga cccgcggaat ctagtggaaa
     2941 tacggatttt gccgatcagg gaaatggtgc cttcctgcga cacgctaatc cgctatcatc
     3001 ggcgccctct tcaagcggct ccactccggc ccctttgaat gcccccttcc gacagtctcg
     3061 tcacataggg gactcggact tggagctaaa catttcctcc atgaagttgg acgacatgga
     3121 cctgtcctct ttcaccactg atagcgaagc cggtgggctg agcaaccgca ttaagctgga
     3181 ctccggggtc tggggatcac agccgttccc caacaaccaa catttgttcc gcgagcctat
     3241 gcgcagcgcc ttcaagccac agcagcagca acatcagcag caccaaccac aacaaatgaa
     3301 gccaccgcag gaagcgaatg ccaactttgc actgaatcca cagccttttc caaaaattac
     3361 aactctcgag gatatagagc gcaatatgat cattcagcag gccattccga aacaacagca
     3421 gcagagccag caacaggcgg aacgcaaaat gtttgatgat tttagtctgg gtaacaggca
     3481 gcaggtgcct acaccaacca tgttgcagca acagatgttg cagcagcagc atcatcagaa
     3541 acaacagtcg cagcagcagc cgccacaaca agcgcagcac aaggtacccc ctggcttctt
     3601 gggcacgccg cacacctctc caccgaggcc aaatttgggt ttccccggac ccgagctact
     3661 gcccacgcca tcgtcgcagc aacaactaaa cggtattggt ggcaaccgag tgccgcccgg
     3721 atttatttac ccgcaaggtc tgcccgggaa tattccgcaa ttgccccagc atcctctaat
     3781 gcagcagcac atgccgcaca actttccact gcccggcggc aaccagcgac ctctacccaa
     3841 tccacaagct ctgcccacgg ccctgaacaa tttcgcgatg catccaagct tcaatgcgat
     3901 gcgcgccgct ggaatccatc caggggcttt tatgcaacct ccccatccgc accagatgct
     3961 gccaccgcga atgccgccgc acccgctgca agctgccaca agtctattag gccagcagcc
     4021 accgaattcc atgtataaca tgttcaacat gcgactagtg caagagatcc agcaaaacca
     4081 tccgctgctt caacaggctg ctgttgtacg ccaaatgcaa cagagcagag caggatccgt
     4141 ggccagcgat cgtcagagat cgcaggcgca acagcagcaa caaggacgac gtccagatgg
     4201 tacaggaaat tacccgctgg aagagtacga tgaatatgcc aacctaatga gcacgcgcga
     4261 caagcactgg ctaatcggaa tccagctttc gcagctgaat acggacacgc cgtatatcga
     4321 cgactattac tacacggtgt tcagggagcg caaggcccaa cagaacgggc aaatgcgcaa
     4381 cagtcaggcg cacaaggaca accagttgaa tcatccgctt actcagccac gcggccatgc
     4441 gcagcttatt ctcgtacagt tgggcaacaa gaatggtact cgcaatggac acggacgtga
     4501 gcgacgcaac tcggagaaca ccaacagcac gtcaggcaac acgaacaatg gatcaggtgg
     4561 taacaacaac aacctgacag gctatatatt ctcaccactg aagttcgaga actcactggg
     4621 aaagctacag tacggaagtg tgacagctcc gcgaaaaatt atcgatgctg acattatgag
     4681 tggtgaatca aatgccgctg ctgaagccaa cgcaacttct tcatcaaaaa gctcgactcc
     4741 gctgcccacc tccgcgagcc atgatgtaaa tcccagtagc atgcggaagt cgcgacacat
     4801 tttgcttctg attgaaacac tgtatcgcta tctgctgaag ttggaggatt tggaaagccc
     4861 agaagtgatg gccaccattg agctgaaaaa gaaaaaggaa gccgaacgga tcgcagccct
     4921 tgagcagctg gaaatggcca ataaaacgcc cgaagaacgt gctgctgaag ccgccaatcc
     4981 gcaaaccatg aatccgcaac taaagaacaa gttcaactat gaggtagaga cgaaagctgc
     5041 gttggtcaat aaattaaagg caggtctggc attcgacaag gttatcgcca tgatgaatgt
     5101 gcgcaaggga aagatactta ttagacgcat aatgcccttt attgcggatc aaagcattcg
     5161 ctgggtcgtg tggagcggcg tcttctgttc cctacaaaca gtggtgaaaa aggacaagga
     5221 cgacgttgaa ggcgtcttgt acgctcttta cccagagttt aaaaaacacg ttaacaaagc
     5281 tactttcgaa ctcctagtga gcatttcatc tgctattacg ctgaatgaca agaaacttgc
     5341 tggaattttt tgcagtcgct ttggaatcct ttcgctggtt gccctgatac ttcgcgccga
     5401 ggaaatctac gtctcgcgaa ctgacgcaac tcttagcgag gagaacaggc aaaagtggcg
     5461 agaatttctg gcacaggttg catcctgcct caatcgcact attcaaaacc agaccatctc
     5521 ggcagccatt gagtcggatg caattcagcc acttatggac cactttgaac ggtttaaaga
     5581 tcttaaactg gatgcactct tagcattaat aactgaagcc aggcatcaaa ttgattaggg
     5641 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
     5701 cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg
     5761 atcagcctcg actgtgcctt ctaaggcctg agctcgctga tcagcctcga tcgaggatcc
     5821 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     5881 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     5941 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     6001 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcagtc
     6061 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     6121 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     6181 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     6241 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     6301 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     6361 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     6421 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     6481 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccag caaaaggcca
     6541 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6601 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6661 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6721 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     6781 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6841 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6901 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6961 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     7021 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7081 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7141 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7201 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7261 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7321 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7381 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7441 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7501 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7561 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7621 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     7681 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7741 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7801 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7861 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7921 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     7981 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8041 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8101 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8161 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8221 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8281 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     8341 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
//
