LOCUS       sequence-288374-        7924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of CG10077-RA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7924)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-CG10077-RA_B
FEATURES             Location/Qualifiers
     source          1..7924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5209..5250
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5260..5277
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5300..5317)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5350..5484
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5374..5393)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5428..5447
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6106..6694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6186..6205)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6865..7656,7657..7725))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7488..7507
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7726..7830)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7898..7916
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttaatga acatgtacaa cggacagatg aacgccttcg gaggcggtgg
     2761 cggcggagga gctggtggcc tagccggcgg tatggtgccc aatcgcatgg gcggtggtgc
     2821 tcccggtggc ggacccggcg gagcgggcat gttccagcgc aatcgctcgg ccccataccc
     2881 aggtttcaat ggtcatggac ccgccaacgg tggacagcga cgcatgaacg gtggtggtcc
     2941 tagcatggga cccggcggac cacgcaacca ggatggattc ggtggtcaga acgggggcca
     3001 gcgctcttcc aatcatggcg ctcacttgcc caagatcgtt tggtctgagg tgaatcttac
     3061 gccattccgc aagaactttt acaagccgtg cgattcggtg ctggccagga ctgtcggtga
     3121 aacagagacc ttcctcacca gcaatgagat caccatcaag ggcgatcagg tgcccacacc
     3181 cagtattgaa ttcgaggagg gcggctttcc cgattacgtg atgaacgaga tccgtaagca
     3241 gggtttcgcc aagccaacgg ccattcaagc ccagggttgg cccattgcta tgagtggacg
     3301 ggatctggtg ggcgtggccc agaccggatc gggcaagacc ttggcgtatg ttctgccggc
     3361 ggtggtgcac atcaataacc agccgcgcct ggagcgcggt gatggaccca tcgccctggt
     3421 cctggctccc actagagagt tggcccagca gatccagcag gtagccattg agtttggcag
     3481 caacacgcat gtgcgcaaca cttgcatctt tggtggagcg cccaagggac agcaggctag
     3541 ggatttggag cgcggcgtgg agattgtaat tgccacacca ggtcgcctaa ttgatttcct
     3601 cgagcgcggc accacctcgt tgaagagatg cacctatctg gtgcttgacg aggccgatcg
     3661 tatgctcgac atgggcttcg agccccagat cagaaagatc atgcagcaga tccgaccgga
     3721 ccgtcaggta ctcatgtggt cagctacctg gcccaaagag gtgcgccagc tggccgagga
     3781 attccttaat aactacatac aggtgaacat tggttcgctt tcgctgagcg ccaatcacaa
     3841 catcctccag atcgtggacg tgtgcgatga gaatgagaaa ttgatgaagc tgatcaagct
     3901 gctaacagac atctcggcgg agaacgagac caagaccatc attttcgtgg aaaccaagaa
     3961 gcgtgtggac gagatcacgc gcaatatctc ccgccagggc tggcgcgcat gcgccatcca
     4021 cggcgataag tcgcagcagg agcgcgactt tgtgctctcg agcttccgca atggccggca
     4081 ctcgatactg gtggccaccg atgtggctgc ccgcggactc gacgttgacg atgtcaagtt
     4141 tgtgatcaac tacgattatc cttcgaactc ggaggactac gttcatcgca ttggacgcac
     4201 cggacgatcc aataacaccg gcaccgccta caccctgttt acgcattcca acgccaacaa
     4261 ggccaacgac ttgatccagg tgctgcgcga ggccaaccag accatcaatc ccaaactgat
     4321 gaacatggcc atgaacggtg gctacaacaa gcgcggtggc ggcatgggtg gcggctaccg
     4381 tggcggcaat ggctatcagg gccgcaatcc ccagatgggc ggtggataca atggcggcaa
     4441 caactaccgc aacaacaatg gtcccggcgc caccatgaat cgcaactcct ttaacggcgg
     4501 cagcgctggc ggaccaccac gcttcgatca gaagccgcgc acttctccgc cgaatcaggg
     4561 cggacagggc ggctaccgtg gccagggagg cggtggctac cagggacaac agcagcagca
     4621 gcagcagcag aacggcggag tgcccttctc tcgtttcaat cccaatgctg cttgcttcga
     4681 gcccaacaag ccgcagaatg gtccgggatc tggaccgcca caggcgcagc accagcagca
     4741 gcaacaggct caggctgccc agcagcaaca cctacttcct gatgcatccg tgcaggcggc
     4801 gatgacggct gcaaactacg gagttgatca gaagcgctcc cgtttctcgc cctacaacat
     4861 gaactttgcc aacatgccac caccagcgat gaaccagcag caggcggcgg cggcccagca
     4921 gcaacagcaa caacagttgc aaactcccca gcagcaacag cagcagcagc aaatgcaaca
     4981 gccacaggcc tatggccagt actcttcaat gtcctccagc atggcaaccg tgtcgctcaa
     5041 cggaggagct gcagcggtgg ccagctctta ccgcgccccg tacgcagtgc ccacgcaggc
     5101 ggcaccctat atgatggcga acggcggcga catctttgcc tatccgccgc ctccgctgcc
     5161 tgtgcagaac taggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc
     5221 cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga
     5281 gtttaaaccc gctgatcagc ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc
     5341 tcgatcgagg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     5401 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     5461 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     5521 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     5581 gattatgatc agtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     5641 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     5701 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
     5761 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
     5821 cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc
     5881 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
     5941 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
     6001 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
     6061 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6121 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6181 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6241 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6301 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6361 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6421 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6481 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6541 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6601 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6661 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6721 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6781 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6841 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6901 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6961 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7021 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7081 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7141 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     7201 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7261 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7321 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7381 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7441 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     7501 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7561 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7621 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7681 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7741 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7801 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     7861 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     7921 tcgt
//
