LOCUS       sequence-288383-        8581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmBrat.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8581)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmBrat_B
FEATURES             Location/Qualifiers
     source          1..8581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5866..5907
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5917..5934
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5957..5974)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6007..6141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6031..6050)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6085..6104
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6763..7351)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6843..6862)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7522..8313,8314..8382))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8145..8164
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8383..8487)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8555..8573
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttaatgg cgtcctcacc gacaccatct ctggactcga tgcggggcgg
     2761 ggcgaactcg attgaatcgt acgaacacgc cggctatcta tcagactctc cgctcacgtt
     2821 gagcggttca tcgccaccgg caagcgattc ggcaatctgc agtgatgagt acacgggcgg
     2881 cagcagcgtt aaatcacgct ctgaggtgac tgtaatcaat ggccatcatc ccatttcggc
     2941 ctccgtatcg tccagctctt cggccagctc ttcatcctgc tcgtcgtcat cgtcctcatc
     3001 ctcgtcttcg tcatcgtcca gctcctcgac cagcggcctc agcggttgcg gcagtaccag
     3061 cagcagtgtg atcagcgcca acaacgttgc gagcagcaat ggaccaggcg ttatcggcag
     3121 caatcttcag tcctcaaaca atggcggcaa ctcgggcatc agcagtctgg tcgtgggtgc
     3181 gggcaaggga agcaacagca gcagcaacag ctcctcgagc aacacatccg ccaatggatc
     3241 tccgccacgt tgcaccgcct gcaagagcaa gtgcagcgat gcggtggcca agtgcttcga
     3301 atgccagagc tacctgtgcg ccaattgtgt aactgctcat gagttcatgc actgcttcaa
     3361 cggacacaat gtgtgcctga ttaagggttt tgaggcgagc accactggca ctccgctgtc
     3421 ggttggatcg cctagcaaca atccggcgtc gaatgagttc aagtacgcca gttcgctgac
     3481 catgatgctg cagcagcagc aacagctcga ctcgcaacaa cagcaacagc agcaacaatt
     3541 gctgccggcg cagcctatgt cgcaactctc gaagattgta ctggcagccg ccgcgcaggc
     3601 aaattcccag gagcaacagc gagaggatag catctatggt tcactgcacc cacaacaaca
     3661 acaacagcag cagcaacagc agcagcgaca actcttctgc ccccgacaca aacaggagct
     3721 gctcaagttc agttgccgca cctgctgcat cctggtgtgc aaggagtgca ttgtactgga
     3781 gcactccaca ggtctccacg aactggagaa cgtgcagtcc cccggaatga caacctctac
     3841 gggatccaca gccaacgagt cagctttgca gactctgctc gccgatatgc gtggcaaaat
     3901 tggcgagatt gtcggcattg ctggaaactc ggaccagaat ctcaccaagg tgaagctgca
     3961 gtaccaaaag gctcacaacg agctgaacga gacgcaccag ttcttcgctt ccatgctgga
     4021 cgagcgcaag accgaattgc tcaaggaact ggagacgctg tacacggcca aggttaacag
     4081 caacaattcg tggcagcaac gctcccgcga cttgatcgac aagggactgg ccacctgcga
     4141 ggcggtcgag cggagtcctg ctccgccatc atccttgctg acagaggccc ttctcctgcg
     4201 caaatccctg gagcaacaac tacagacggg cattcaggag atgcagctac cattcgagat
     4261 cgaatttatg tccaactatc agtcgattca ggccggcgtg cgcaacacct ttggttacat
     4321 tcgggcaaac agctcagatg gtggacctac tggcatgagc ctgacgagca atggccacgg
     4381 aaagcagccg ccaattgccc gaccaacaca gagcgccagc aacagcagtg cgagcagcgc
     4441 gggcagtggt caccatggcc accaccaaca gtcacaccat catggccacc acaatcatca
     4501 ccagacggcc catcatcaac agctgcaggc gcaatcgtcg ctgcatggct tgggtcttgg
     4561 attgagtggc gccagtttgt tggatagcag ctctagcgca ggcggagcag tgggagcctt
     4621 cagcaacgga ggacttctgc tgggcggtcg cgatcggaac gctctggcag tggaacagca
     4681 tttcggtgag ctgatgccca agcgaggagg aggtggctac acgggcagca atggatcggc
     4741 caccagtgcc gtggcccact acaatcccta cgagaagtgg agcaacggcg gtagcgacaa
     4801 tctgttttca tcggtgacaa gcggcgtgtc cggtagctct gcagtggccg atgcatttgc
     4861 cagcctgtct gctgttggag gatccgtggt tagtggcgct ggagctggcg gaagtactgt
     4921 gagctcagag tcgttgctgg acctcaccaa caagctgctt tcggccacta tctatccgcc
     4981 caagtcgcag atcaagcgac agaagatgat ttaccactgc aagtttggcg aattcggcgt
     5041 gatggagggc cagttcacgg agcccagcgg tgtggcggta aatgcccaga atgacatcat
     5101 tgttgcggat acgaacaacc atcgcattca gatcttcgac aaggagggac gcttcaagtt
     5161 ccagtttggc gagtgcggca agcgtgactc gcagctgctc tatccgaacc gcgtggccgt
     5221 ggtgcgcaat tccggcgata ttattgtcac cgagcgctcg cccacacacc agatacagat
     5281 ctacaatcag tacggccagt ttgtgaggaa attcggggcc accattctgc agcatcctcg
     5341 cggcgtgacc gtggacaaca agggacggat cattgtggtg gaatgcaagg tgatgcgtgt
     5401 gatcatcttt gatcagaacg gcaatgtgct gcacaagttc gggtgctcta agcacctcga
     5461 gttccccaac ggcgtggtgg tcaacgacaa gcaggagatc ttcatcagcg acaatcgggc
     5521 gcactgtgtc aaggtgttca actacgaggg ccagtatctg cggcagattg gcggcgaggg
     5581 tattaccaac tacccaattg gcgtcgggat caactcgaac ggggagatcc tcatcgcgga
     5641 caaccacaac aacttcaacc tgacgatctt cacgcaggac gggcagctga tctcggcgct
     5701 cgagtcgaag gtgaagcacg cgcagtgctt cgatgtggcg ctcatggacg acggcagtgt
     5761 ggtgctggcc agcaaggact atcggctcta catatatcgc tatgtccaac tggcgccagt
     5821 gggtatgtaa gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct
     5881 aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt
     5941 taaacccgct gatcagcctc gactgtgcct tctaaggcct gagctcgctg atcagcctcg
     6001 atcgaggatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     6061 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     6121 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     6181 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     6241 tatgatcagt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     6301 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     6361 acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac
     6421 gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt
     6481 cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc
     6541 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat
     6601 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag
     6661 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcca
     6721 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6781 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6841 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6901 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     6961 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7021 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7081 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7141 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7201 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7261 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7321 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7381 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7441 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7501 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7561 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7621 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7681 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7741 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7801 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     7861 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7921 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7981 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8041 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8101 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     8161 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8221 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8281 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8341 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8401 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8461 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     8521 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     8581 t
//
