LOCUS       sequence-288389-        7674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPenguin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7674)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmPenguin_B
FEATURES             Location/Qualifiers
     source          1..7674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4959..5000
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5010..5027
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5050..5067)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5100..5234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5124..5143)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5178..5197
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5856..6444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5936..5955)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6615..7406,7407..7475))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7238..7257
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7476..7580)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7648..7666
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgatgg ttagctcgga gccgaaagga cctgctaacg tggccaacac
     2761 ggcctcgccc ctcaaggccc atgaatcgtc cggcaagcgc gccaacggac agaagtttaa
     2821 gcccggtgga gcaggaggcg ccaggaagtt cgacaaattc ggcggaggag caggcaagtc
     2881 gtttgtaaag ccaggcgctg gtggtgccaa gaagttcgac aagtctgggc ccggtggctt
     2941 caagaagttt gacaagtccg gagccaccgg cgacaagagg cttggtggca acaagttccg
     3001 cggaaaacca caaacacagc cacaggcgcc ggcggagggc gagaaacagg actggaacaa
     3061 gttcaagaag gagaagaagg acctcaagct gaagcgcaag agtgccaagg acacctacga
     3121 gatcagcaag gaggccaacc agatccacga gaagttgcga tgccgccgaa cggagaacaa
     3181 ggacaagctg gtggagcaga tatacaaggt gctcaacgtg ggcgacacta tttcaaaggt
     3241 ggtgaaggcc cacgacaccg cccgcgtgat ccagagcatg ctaaagtacg cgtcgccagc
     3301 tctgcgggcc gagatctctg agaagctgct tcccttcacc gtggagatgt gccagtcgaa
     3361 atacgcccag ttctgcgtgc aacggatgct caagtacggt gcgccagcca ccaaagccaa
     3421 gctggtggac agcctgtacg gacatatagt gcgtttggcc ggacacagca tcggcagtgg
     3481 gctgctggat tcgatgtatc agagcgccac acccaatcag cgcatctata tgcgccagga
     3541 gttctacggc gatctgtacc ggaaggccaa ggattcgaat gtgaagactc tgagcgacac
     3601 ctataaggag gccacgaaca tgaaggcctc cattttgggg tcggttaaag caaacctgga
     3661 tcatgtggcc aacaagcagc tggtggacag tgcgctggtg cacgccgtga tgctggagta
     3721 cctgcgcgcc tgcgacgaag atgaggagaa gctggaggag acggtcactg cgtttgcggc
     3781 tctagtgcct cacatgctgt ccaccaagga gggcagcgag gcggctgtta tatgcttcta
     3841 caagtcgacg cccaagaatc gcagagccat tatcaagaat atcaaggagc acctgctaaa
     3901 gatcgccaac catgagcacg gccacgtgtt cctcatctcg ctgctaaacg cactggacga
     3961 cactaaggcg accaagaagg caatttacga tcacctacac ggagatctaa aggcgctaat
     4021 gagcagcccg tacggccgac gagtgattca atggctggtg gctccgggtg atacgacatg
     4081 cttccatcca gagttcattc gcacggtgga ggagggtcta gcctttggaa aaaaggagaa
     4141 ggagctgcgt cgcaaggaga tccttgagca gatagaggcg ccgattgcac agtccatcgc
     4201 cgaggatgcc gccttttggc tgtccaacag ccacatcggc ctggtcaccg gcgatattct
     4261 taatcacatt caaggcgagt cctatgagaa ggctgcttcg gcccttgccc aagtcgtcgt
     4321 ccaaccggag tggcgcattt cagcagacgc cgccggtccg cagccgcagg ataagaagaa
     4381 gcctcacaat gacgtggagg ccattatagc ccaggccacc aaacagcgca gaaaactgct
     4441 ttatgtcgag agcagcagcg acgacgaaga tgaagatgaa gatgaagatg aggagagcga
     4501 cgatgaagga gacgagaagg agcagaagga ggcggccgca gatgatgccg aacccaaggt
     4561 gaagaaagcc aaaaaggagc ccaaaaagcc aaaggcaaag gaggaggagc cggcggctcc
     4621 gttggtctcc ggaatcgagg aggccggcat gcacattgta ttgaagaaga tactcaagaa
     4681 cgatggcaag cgagagggca ccccgttcag ccaacagcta ctccaaaact tgtcctccga
     4741 tgtgctgaaa gcctggctgg gcgtcaaccg ggcctgtttt gtcctgctca agctggtgga
     4801 ggagtgtccc gcactgctgg acgactgcaa gaaagccatc gcggcggaga ggagccttag
     4861 ccagatactc gcggaccgta agacgcccgg agccaagcta ctggccgcca aactggacat
     4921 tggcaaatga ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     4981 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc
     5041 gctgatcagc ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg
     5101 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     5161 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     5221 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     5281 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     5341 agtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     5401 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     5461 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     5521 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     5581 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     5641 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     5701 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     5761 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag ccagcaaaag
     5821 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5881 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5941 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6001 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
     6061 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6121 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6181 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6241 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     6301 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6361 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6421 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6481 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6541 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6601 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6661 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6721 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6781 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6841 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6901 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     6961 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7021 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7081 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7141 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7201 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     7261 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7321 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7381 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7441 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7501 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7561 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     7621 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgt
//
