LOCUS       sequence-288390-        8259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPumilio.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8259)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmPumilio_B
FEATURES             Location/Qualifiers
     source          1..8259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5544..5585
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5595..5612
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5635..5652)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5685..5819
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5709..5728)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5763..5782
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6441..7029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6521..6540)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7200..7991,7992..8060))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7823..7842
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8061..8165)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8233..8251
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgatgg tggttttaga aactgccagc gcccttttgg gcggcccgta
     2761 tgcccagggc gctcccgccc tgaaaatggt gcagaaacgc tacattggat tacatcactg
     2821 gctgggacca atccgctcca aggagctaaa ggagcatatt gtaagtgacg acgtgctttc
     2881 gctcgctttt aaccacaatc aacaactgtt tcgctcgcag aatccgggcc tagcagcagt
     2941 tgccacaaat gcagcggccg cagcagcagc cgcagcagct gccacatcgg cagcgagtgc
     3001 tgcggcagcg gtgggcgcac cacccgttcc caacggatcg ctgcagcagt cgcagcagca
     3061 acagcagcag cagcaacaac agcagcagca acaacagatg cacatggcgg ccgcgtcgca
     3121 acaatttttg gccgcccagc agcaggcgca aaatgcggcc tatgccgccc aacaggccac
     3181 gtcctacgtc atcaatccgg gccaggaggc tgccccgtat atgggcatga ttgccgccgc
     3241 ccagatgccg tactatggcg tagcaccatg gggcatgtat ccgggcaatc tgattccgca
     3301 acagggaacg cagccgcgcc gccccctcac cccctcgcag cagggtgccg agaatcagcc
     3361 gtatcaggtc atcccggcat tcctcgatca cacgggctcc ttgctgatgg gaggacctcg
     3421 caccgggacg ccgatgcgtc tggttagccc cgcccccgtt ctggtgcccc cgggcgctac
     3481 ccgtgccggc cccccgcccc cgcagggccc acagctgtat cagccgcagc cgcagacggc
     3541 ccaacagaat ctctactcgc agcagaatgg atccagtgtc ggaggcctcg ccttgaacac
     3601 gagctcgttg acgggtcgcc gcgactcctt cgaccgcagc acctccgcct tcagtccctc
     3661 gaccatggac tacaccagca gcggtgtggc agcggccgcc aatgcggtga acagcacagt
     3721 ggcccaggca gcagcagctg ccgcagcagc cgccgcagcg cgtggcaagt ggccgggagc
     3781 gatgtcggga gcggccagtg gagcctacgg agccctggga gcgggcaatg cttcggccag
     3841 tcccctgggc gcaccaatca cgccgccgcc atcggcgcaa tcctgtctcc tgggcagtcg
     3901 ggcacctgga gccgagtccc gccagcggca gcagcaacaa cagcagctgg ccgccgttgg
     3961 tctgccggcg actgcagcag ctgctcaggc agcggtggcc gcggctgcca acaatatgtt
     4021 cggatccaac agctcgatct tctcgaatcc cctggccatt ccgggtaccg cagctgtggc
     4081 agctgcagcg gcagcagcgg ccgccaactc gcgtcaggtg gctgccacgg cagcggcagc
     4141 agcggctgtg gcagcagcag ccggcggagt gggaggtgcc ccacagccag gaagatctcg
     4201 ccttctcgaa gatttccgca accagcggta tccaaatctt cagctacgcg atctcgctaa
     4261 ccacattgtg gagttctcac aggatcagca cggctcgcgg tttatccaac agaagttgga
     4321 gcgggccacc gccgccgaga agcaaatggt gttcagcgag atcctggcgg cagcctatag
     4381 cctgatgacc gatgtctttg gcaactatgt catccagaag ttctttgagt tcggcactcc
     4441 cgagcagaag aacacgctgg gcatgcaggt caagggtcat gtgctgcagc tggcgctgca
     4501 aatgtatggc tgccgagtga ttcagaaggc tctggagagc atctcgccgg agcagcagca
     4561 ggaaatcgtc cacgaactgg acggacatgt gctgaaatgc gtcaaggatc agaatggcaa
     4621 tcatgtggtg caaaagtgca ttgagtgcgt ggaccccgtg gcgctgcagt tcatcatcaa
     4681 tgctttcaag ggtcaggttt actcgctaag cacccatccg tatggatgcc gggtgatcca
     4741 gagaatcctt gagcattgca ctgccgaaca gaccacgccc attttggacg aactgcatga
     4801 gcacaccgaa cagttgattc aggaccaata tggcaactat gttattcagc atgtgcttga
     4861 acacggcaag caggaggata agtcgattct tatcaacagc gtgcgcggca aagttctggt
     4921 gctatcacag cacaagttcg cctcaaacgt tgtggagaaa tgtgttaccc atgccactcg
     4981 cggagaacgc actggtctca tggacgaggt ctgcaccttc aacgacaacg cgttgcacgt
     5041 gatgatgaag gatcagtatg ccaactatgt ggtccaaaaa atgatcgatg tatcggagcc
     5101 gacgcagctc aagaagctga tgaccaagat ccggccccac atggccgcct tgcgcaagta
     5161 cacctacggc aagcacatca atgccaagtt ggagaagtac tacatgaaga taaccaatcc
     5221 cattacggtg ggcacaggag ctggaggagt gccggcagcc tcgtcggcgg ccgcagtcag
     5281 cagtggtgcc acctcggcat cggtaaccgc ctgcaccagt ggcagcagca ccaccacgac
     5341 cagcactacc aacagcctgg cctcacccac catttgttcg gtgcaggaga acggcagcgc
     5401 catggttgtg gagccctcct ccccggacgc ctccgagtcc tcgtcctcgg tggtgtcagg
     5461 cgctgtcaac agcagcttgg gtcccattgg acccccgacc aacggcaacg ttgtgctgta
     5521 atctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt
     5581 ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga
     5641 ctgtgccttc taaggcctga gctcgctgat cagcctcgat cgaggatcca gacatgataa
     5701 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     5761 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     5821 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     5881 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtcg accgatgccc
     5941 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     6001 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
     6061 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
     6121 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
     6181 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
     6241 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
     6301 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     6361 ggacagcttc aaggatcgct cgcggctctt accagccagc aaaaggccag gaaccgtaaa
     6421 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6481 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6541 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6601 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     6661 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6721 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6781 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6841 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     6901 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6961 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7021 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7081 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7141 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7201 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7261 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7321 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7381 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7441 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7501 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7561 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7621 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7681 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7741 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7801 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7861 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7921 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7981 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8041 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8101 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8161 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     8221 ttaacctata aaaataggcg tatcacgagg ccctttcgt
//
