LOCUS       sequence-288422-        8326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmAGO1-RB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Behm-Ansmant I, Rehwinkel J, Doerks T, Stark A, Bork P, Izaurralde E
  TITLE     mRNA degradation by miRNAs and GW182 requires both CCR4:NOT 
            deadenylase and DCP1:DCP2 decapping complexes.
  JOURNAL   Genes Dev. 2006 Jul 15. 20(14):1885-98.
  PUBMED    16815998
REFERENCE   2  (bases 1 to 8326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2006 Jul 15. 20(14):1885-98."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmAGO1-RB_B
FEATURES             Location/Qualifiers
     source          1..8326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5611..5652
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5662..5679
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5702..5719)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5752..5886
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5776..5795)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5830..5849
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6508..7096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6588..6607)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7267..8058,8059..8127))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7890..7909
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8128..8232)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8300..8318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ccatgtatcc agttggacaa cagtcacagt ggaccccctc
     2761 gcccactcgg cctcagagtc cctctcaggc gcagactagc ttcgacacgc tcacatcacc
     2821 accagcgccc ggctcgtcgg tcaatcccac cgccgtcacc agcccaagtg cccagaatgt
     2881 ggccgctggt ggagcaactg tggccggtgc agcagcaact gccgcccagg tggcctccgc
     2941 cttgggtgcc accaccggca gcgtgacgcc agcaattgcc accgcaacgc cagccacgca
     3001 gccggatatg cccgtcttta cgtgtccacg acgcccgaat ctcggacgag agggtcgccc
     3061 gattgtgctg cgcgccaatc acttccaggt gacaatgccg cgtggctatg tgcatcacta
     3121 tgacatcaat atacagccgg acaagtgtcc gcgaaaggtg aaccgtgaga ttatcgagac
     3181 tatggtgcat gcctatagca agatcttcgg agtgctcaag ccggtgttcg atggtcgcaa
     3241 caatctgtat acccgcgatc ccctgcccat tggcaacgag cgtctagagc tggaggttac
     3301 tctacccggc gagggcaaag atcgaatctt tcgcgtgacg atcaagtggc aggctcaggt
     3361 ctcgctcttc aatttggagg aagctctcga aggccgcacg cggcagatac cctatgatgc
     3421 cattttggcg ctcgatgtgg tcatgcgcca tctgcccagc atgacgtaca cgccagtggg
     3481 acgtagcttc ttcagttccc cggagggtta ctaccatccc ctgggtggtg gacgcgaggt
     3541 ttggttcggt ttccatcaga gcgtaaggcc ctcgcagtgg aagatgatgc tcaatatcga
     3601 tgtctcggcc accgctttct acaaggctca accagtcatt gacttcatgt gcgaggtgct
     3661 ggacattcgc gacatcaacg agcagcgcaa accgctcacc gattcgcagc gcgtcaagtt
     3721 cacgaaggag atcaagggtt tgaagatcga gatcacccac tgcggccaga tgcgtcgcaa
     3781 gtatcgtgtg tgcaacgtca ctcgccgccc cgctcagatg caatcattcc cactgcagct
     3841 ggagaacgga cagaccgtag agtgcaccgt ggccaagtac ttcctggaca agtaccgcat
     3901 gaaattgcgc tacccgcact tgccctgcct gcaggttggc caagagcaca agcacactta
     3961 cctgcctcta gaggtgtgca acattgtggc cggacagcgg tgcattaaaa agctgaccga
     4021 tatgcagacg tcgaccatga tcaaggccac agctcgttca gctccggatc gtgagcgtga
     4081 gattaacaac ttggtaaagc gcgccgactt caacaacgat tcgtatgtgc aagagtttgg
     4141 cctgaccatc tccaattcga tgatggaggt acgaggacgc gtcttgcctc ctcccaagct
     4201 tcagtatggg ggacgtgtgt ctaccggcct caccggccag cagctgttcc cgccacagaa
     4261 caaggtgagc ttggcctcgc ccaaccaggg tgtatgggat atgcgaggca agcagttctt
     4321 cactggcgtc gagatccgca tctgggccat cgcctgtttt gccccacagc gcacggtgcg
     4381 cgaggatgcg ctgcgtaatt tcacccagca gctgcagaag atctcaaacg atgcaggcat
     4441 gccgataatt ggacagccgt gcttctgtaa gtacgccacc gggccggatc aagtggaacc
     4501 catgttccgt tacctgaaga tcaccttccc cggcctgcaa ctcgtcgtgg ttgtgctgcc
     4561 cggcaagact ccagtatacg ccgaggtgaa gcgtgtaggt gacaccgttc tgggtatggc
     4621 cacccagtgt gtgcaggcca agaacgtgaa caagacatcg ccacagacgc tctctaatct
     4681 gtgtctgaag atcaacgtca agttgggcgg catcaattca attctggtcc cctccattcg
     4741 gccaaaggtc ttcaatgagc cggtcatctt tttgggtgcc gatgtgacac acccaccagc
     4801 tggcgacaac aagaaaccat cgattgccgc cgtcgtgggc tccatggatg cccatccatc
     4861 gcggtatgcc gccaccgttc gcgtacagca gcaccgacag gagatcatcc aggagctgag
     4921 cagcatggtg cgcgagctgt tgatcatgtt ctacaagtcg acgggcggct acaagcccca
     4981 ccgcatcata ctctatcgtg acggagtctc cgagggacaa ttcccacatg tcctgcaaca
     5041 cgaattgacc gccattcgcg aggcctgcat taagctagag ccagagtatc ggccgggcat
     5101 cacattcatt gtggtgcaga agcgccatca cactcgactc ttctgcgcgg agaagaagga
     5161 gcagagcggc aaatcgggca atattcccgc aggcaccacc gtcgatgtgg gcatcacaca
     5221 tcccaccgaa tttgatttct atctatgcag ccatcagggc atccagggca ccagtcgccc
     5281 ctcgcactac cacgttctgt gggacgacaa tcactttgac tcggacgagc tgcagtgcct
     5341 cacgtatcag ctatgccata cgtacgtgcg ctgcacccga tccgtcagta taccggcgcc
     5401 agcctactac gcccatttag tggccttccg tgccagatat catctggtgg agaaggagca
     5461 cgattcgggc gagggttcgc accagagcgg ctgctcagag gatcgtacgc caggtgccat
     5521 ggccagggcc atcactgtgc acgcggatac caagaaggtc atgtactttg cctaagcggc
     5581 cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt
     5641 ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
     5701 gcctcgactg tgccttctaa ggcctgagct cgctgatcag cctcgatcga ggatccagac
     5761 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     5821 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     5881 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     5941 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcagtcgacc
     6001 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     6061 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     6121 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
     6181 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     6241 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     6301 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     6361 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     6421 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agccagcaaa aggccaggaa
     6481 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6541 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6601 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6661 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
     6721 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6781 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6841 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6901 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     6961 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7021 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7081 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7141 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7201 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7261 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7321 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7381 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7441 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7501 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7561 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7621 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7681 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7741 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7801 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7861 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     7921 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7981 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8041 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8101 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8161 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8221 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     8281 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgt
//
