LOCUS       sequence-288426-        9187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmAGO2-RC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Rehwinkel J, Natalin P, Stark A, Brennecke J, Cohen SM, Izaurralde E
  TITLE     Genome-wide analysis of mRNAs regulated by Drosha and Argonaute 
            proteins in Drosophila melanogaster.
  JOURNAL   Mol Cell Biol. 2006 Apr . 26(8):2965-75.
  PUBMED    16581772
REFERENCE   2  (bases 1 to 9187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2006 Apr . 26(8):2965-75."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmAGO2-RC_B
FEATURES             Location/Qualifiers
     source          1..9187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6472..6513
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6523..6540
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6563..6580)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6613..6747
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6637..6656)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6691..6710
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7369..7957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7449..7468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8128..8919,8920..8988))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8751..8770
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8989..9093)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9161..9179
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catgcacttt ccaattacca ccccagagaa
     2761 caagaaagga ggacaggata gcgctgcagc accacagccc cagcagcagc aaaagcaaca
     2821 acaacaacgg cagcaacaac cacagcagct gcaacaacca cagcagctgc aacaaccaca
     2881 gcagcagcaa caacaacaac cgcatcagca acaacaacaa agttcgagac aacagccatc
     2941 cacaagctca ggcggatccc gtgcatctgg gttccagcaa ggaggccaac agcagaaatc
     3001 ccaagacgca gaaggatgga ctgcacagaa aaaacaaggc aaacagcagg tacaagggtg
     3061 gactaaacaa ggtcaacaag gtggccatca gcaaggacga caagggcaag acggtggcta
     3121 ccaacagcgt ccgcctggac aacaacaagg tggccatcag caaggacgac aagggcaaga
     3181 aggtggctac caacagcgtc cgcctggaca acaacaaggt ggccatcagc aaggacgaca
     3241 agggcaagaa ggtggctacc aacagcgtcc gtctggacaa caacaaggtg gccatcagca
     3301 aggacgacaa gggcaagaag gtggctacca acagcgtccg cctggacaac aacaaggtgg
     3361 ccatcagcaa ggacgacaag ggcaagaagg tggctaccaa cagcgtccgt ctggacaaca
     3421 acaaggtggc catcagcaag gacgacaagg gcaagaaggt ggctaccaac agcgtccgcc
     3481 tggacaacaa caaggtggcc atcagcaagg acgacaaggg caagaaggtg gctaccaaca
     3541 gcgtccgcct ggacaacaac aaggtggcca tgagcaagga cgacaagggc aagaaggtgg
     3601 ctaccaacag cgtccgtctg gacaacaaca aggtggccat cagcaaggac gacaagggca
     3661 agaaggtggc taccaacagc gtccgtctgg acaacaacaa ggtggccatc agcaaggacg
     3721 acaagggcaa gaaggtggct accaacagcg tccgtctgga caacaacaag gtggccatca
     3781 gcaaggacga caagggcaag aaggtggcta ccaacagcgt ccgtctggac aacaacaagg
     3841 tggccatcag caaggacgac aagggcaaga aggtggctac caacagcgtc cgcctggaca
     3901 acaaccaaac caaacccaga gccaaggcca ataccaatct cgtggaccac ctcagcaaca
     3961 gcaggctgcc ccattaccat taccgcctca gccagccggg agcattaagc gcggaacaat
     4021 cggcaaaccc gggcaagtag gcatcaacta tctggacctt gacctgtcca aaatgccttc
     4081 tgtggcatac cactatgacg tgaagatcat gccagagcgt cccaaaaagt tttacagaca
     4141 ggcatttgaa caatttcggg tggaccaatt gggcggagca gttcttgcct atgatggcaa
     4201 agcctcttgc tactcggtgg ataagctgcc gttaaatagc caaaatccgg aagtgactgt
     4261 gacagatcgt aatggtcgta ctttgcgcta cacgatcgaa atcaaggaga ctggtgactc
     4321 gaccattgac cttaaatcgt taactactta tatgaatgac cgaattttcg acaagcccat
     4381 gcgagcaatg cagtgcgtgg aggttgtttt ggcttcaccc tgccacaaca aagccatccg
     4441 tgtcggtcgt tccttcttta agatgtccga tcctaataat cgtcacgaac tggatgatgg
     4501 atacgaggcc ctggtcggtc tgtatcaggc atttatgctg ggcgataggc catttttaaa
     4561 tgtagatata tcgcacaaat cctttccgat ttcaatgccg atgatcgaat atttggagcg
     4621 atttagctta aaggctaaaa tcaacaatac aacgaattta gattattcgc gacgcttcct
     4681 tgagccattc ctcagaggca ttaatgtggt ttacacgcct cctcagtcgt ttcaaagcgc
     4741 tccaagggta taccgtgtaa atggtctttc tcgtgccccg gccagcagtg agaccttcga
     4801 acacgatggg aagaaggtca ccattgcgtc ctacttccac agtcgcaact acccattgaa
     4861 gtttccccaa ctccattgtc tgaacgttgg atcttcaatc aagagtattc tgctgcccat
     4921 cgagctatgc agcatcgagg aaggtcaggc cctaaaccgc aaggatggag caactcaggt
     4981 ggccaatatg ataaagtacg cagccacatc gacgaacgtg cgaaagcgca agattatgaa
     5041 cttgctgcaa tacttccagc acaacctgga tccgaccatc agtcgctttg gcatccgcat
     5101 tgccaacgat tttattgtgg taagcacccg cgtcctaaac ccacctcagg ttgaatatca
     5161 tagtaagagg tttactatgg tgaagaacgg gtcgtggcgc atggatggca tgaagtttct
     5221 ggagcccaag cccaaggcgc acaagtgtgc ggtcttgtat tgcgatccga ggagtggtcg
     5281 caaaatgaac tatgcccagc tgaatgactt cgggaaccta ataatatccc aaggcaaggc
     5341 agtcaacata agcttggatt ctgatgtgac atacagaccg ttcacggatg acgaacgcag
     5401 cctagacact attttcgcgg atctgaagcg cagccagcac gatctggcaa ttgtgattat
     5461 tcctcagttt agaatttctt acgatacaat taagcagaag gccgagctgc agcatggaat
     5521 tttgacgcaa tgcattaagc agttcaccgt ggaacgaaag tgtaataatc agacgattgg
     5581 aaatattcta cttaagatca actccaagct gaacggcatc aaccacaaga tcaaggatga
     5641 tcctcgtctg ccgatgatga agaacaccat gtacattgga gccgatgtga cccatccctc
     5701 tcccgatcag cgcgagattc ccagtgtggt cggagtagca gcctcacacg atccctacgg
     5761 agccagttat aacatgcaat atcgtttgca gcgaggggct ctggaggaga ttgaggacat
     5821 gttctcgatt actttggagc acttgcgcgt gtataaagag taccgtaacg cctatcctga
     5881 tcatatcatc tactaccgag atggcgtgag cgacggccag tttccgaaaa tcaaaaacga
     5941 ggaactgagg tgtattaaac aagcctgtga caaggtgggc tgtaaaccca agatttgctg
     6001 cgtgattgtg gtgaagcgtc atcacactcg cttctttccc agcggcgacg taacgacatc
     6061 gaacaagttc aacaacgtgg accccggaac cgtggtcgat cgcaccattg tgcatcctaa
     6121 cgagatgcag ttcttcatgg tcagccacca ggccatccag ggcacggcca agccaacacg
     6181 atacaatgtg attgagaaca caggcaatct tgacatcgac ttgttgcagc agttgaccta
     6241 caacctgtgc cacatgttcc ctcgttgcaa tcgctcggtt tcttatccgg ctccggccta
     6301 tttagcccat ttggtagctg ctcgtggacg cgtttatctg actggcacca acaggttcct
     6361 ggatttgaag aaggagtacg caaagcgaac gattgtcccc gaattcatga agaaaaaccc
     6421 catgtacttt gtctgagcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct
     6481 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat
     6541 tgagtttaaa cccgctgatc agcctcgact gtgccttcta aggcctgagc tcgctgatca
     6601 gcctcgatcg aggatccaga catgataaga tacattgatg agtttggaca aaccacaact
     6661 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     6721 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     6781 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     6841 gctgattatg atcagtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     6901 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     6961 cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag
     7021 cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc
     7081 cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat
     7141 ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt
     7201 ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct
     7261 gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac
     7321 cagccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7381 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7441 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7501 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7561 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7621 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7681 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7741 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7801 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7861 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7921 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7981 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8041 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8101 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8161 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8221 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8281 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8341 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8401 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc
     8461 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8521 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8581 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8641 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8701 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc
     8761 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8821 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8881 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8941 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9001 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9061 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9121 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     9181 ctttcgt
//
