LOCUS       sequence-288451-        8525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmSmaug-del584-762.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8525)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmSmaug-del584-762-V5His6_C
FEATURES             Location/Qualifiers
     source          1..8525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5810..5851
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5861..5878
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5901..5918)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5951..6085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5975..5994)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6029..6048
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6707..7295)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6787..6806)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7466..8257,8258..8326))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8089..8108
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8327..8431)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8499..8517
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgaattctg cagatatcat gaagtacgca actggaactg acaacgcgat
     3361 gacctccggc atcagcggcc aaaccaataa ctctaacagt gcatccaacg agatgcagcc
     3421 aaccacatca acgcctactg cagcccacaa ggaggccaca tcaacggcca cgacgacggc
     3481 gacatacgcc aatggcaatc ccaattctaa cgccaatccc agccagagcc agccgtccaa
     3541 tgcgctgttt tgcgagcagg tgaccacagt gacaaatctc ttcgagaagt ggaacgactg
     3601 cgagcgaacc gtcgtgatgt acgcgctgct caagcggctg cgctacccga gcctcaagtt
     3661 tctacagtat tctatcgaca gcaacctgac ccagaacctg ggcacctcgc agaccaatct
     3721 gagcagcgtg gttattgaca tcaatgccaa caatccggtg tacctgcaga atctactcaa
     3781 cgcctacaag acgttccagc catgtgacct gctcgacgcc atgtcctcct cgtcgtccga
     3841 caaggactcg atgccatgtt acggcagtga cttccagatc accacatcag cgcagtgtga
     3901 cgagcgaaag ctgtatgccc gcaaggagga cattctgcat gaagtactga acatgctgcc
     3961 tctgctgaag ccgggcaatg aggaggccaa gcttatctac ctgaccctca taccagttgc
     4021 cgtcaaggac accatgcagc aaattgtgcc cacggagttg gtgcagcaga tcttctcgta
     4081 cctactcatc catccagcta tcaccagcga ggacagacgt tcgctcaaca tttggctgcg
     4141 tcacttggag gatcatatcc aagcggctgc ggcgggcctg acaaatcgca gttacttcct
     4201 gcagccctcg ccgcaactgg tcgctggtgg tagctcaaca ggcagtggta gctgttcctc
     4261 ttcggcgacc agctcttcga cagcatcctg ttcgtcggtg gcctcatcct cgttgtgccc
     4321 cgcaagcggc agccgttcct cacgcaccaa cgactggcaa acgatcgccc cgcccagcaa
     4381 gcaattgcaa aacaaattgg ccggtgattg gcgtggcaat ggaggcggca gctccagcgg
     4441 gtcgatcaat ccactctgtg ataatcttaa tggtattacc ctgaacgaat tagcttctag
     4501 tcagaacagt ctcggcctgt cgctggaggg cagctcttcc ctggtcaacg gtgtggtcgc
     4561 tggtgcaggc tctatgttgg gaatcgctgg tggcgacgat catgacacgt catttagcaa
     4621 gaatggcacg gagattctcg attttgaccc ggtgacggcg gacatgggtg aggcctgtag
     4681 tctggccagc agtagccttt gtggacgtaa cggtggaaat cctgttgagg atcgatcgca
     4741 acctccgccg aatcttcagc agcaattgct tcagcctcct ccctatgcct cgatccttat
     4801 gggaaacgtg ggcgatcagt ttggagagat caaccggtgg agcctggaca gcaagattgc
     4861 agccctgaag acgcggcgct ctaacagcct caccacgcaa acgatctcta gctgttccag
     4921 ctcgtctaac tcatcggtga tcacggtcaa tgacaactgc tccaattcaa cggagaatct
     4981 ggcacagttt gccaacaagc cccgtagttt ttccttatcg attgagcatc agcgcggcgc
     5041 cttgatgaac agcggttccg atactcgtct ggatgaaatg aagatctcca tggttcccaa
     5101 aatgcatcac gttaagccag ccggcgtcgg gccaaacaat gggaacatta acaagccaag
     5161 atggaatggt aagactcgca aatgcgatac taaaaatggc agcaacgata ggattaacaa
     5221 ccgcaaaaac tccaacgaca tgctaaactt ttcgctaaat tgcctgccac acccgctgcc
     5281 ccatcattcg cagcaggctc cgccgcctct gccacagttt gactacaacg gctatggcgg
     5341 cggtccatcc catcagccgc agtacaagag ctcctcgtac ccaagcttca tgggtaatcc
     5401 tcagcagcag ccaccaccgc cgccgtctag taagtcgcat catcatcccc agcagatgca
     5461 acagatgctg cagcaacaca accactttcc agcgctgcct cagcagacgc caccacaatc
     5521 gcatcgccgg tcgctgaata atcttattct ggtggccgga ggtccgcagc aaccgcagca
     5581 attgatcttt aagcccggtc agggagtgct caccaacaac ggatctaacg acaacctggt
     5641 attggaacgc aatcagcagt cgcagcagca gagaaaactt agcggaggag tgtccagtgc
     5701 ggagcaacaa ccaaagaaga ccatggccgc tgttgtgatg gaaaatttgg ccaaatttga
     5761 tcaacatttt acgctattcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
     5821 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg
     5881 agtttaaacc cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc
     5941 ctcgatcgag gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     6001 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     6061 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     6121 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     6181 tgattatgat cagtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     6241 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     6301 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
     6361 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
     6421 tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
     6481 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
     6541 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
     6601 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca
     6661 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6721 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6781 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6841 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6901 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6961 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7021 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7081 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7141 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7201 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7261 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7321 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7381 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7441 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7501 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7561 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7621 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7681 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7741 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     7801 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7861 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7921 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7981 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8041 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     8101 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8161 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8221 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8281 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8341 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8401 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8461 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     8521 ttcgt
//
