LOCUS       sequence-288454-        7226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmSmaug_1-583.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7226)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmSmaug_1-583-V5His6_C
FEATURES             Location/Qualifiers
     source          1..7226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4511..4552
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4562..4579
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4602..4619)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4652..4786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4676..4695)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4730..4749
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5408..5996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5488..5507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6167..6958,6959..7027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6790..6809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7028..7132)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7200..7218
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catgaagtac gcaactggaa ctgacaacgc
     2761 gatgacctcc ggcatcagcg gccaaaccaa taactctaac agtgcatcca acgagatgca
     2821 gccaaccaca tcaacgccta ctgcagccca caaggaggcc acatcaacgg ccacgacgac
     2881 ggcgacatac gccaatggca atcccaattc taacgccaat cccagccaga gccagccgtc
     2941 caatgcgctg ttttgcgagc aggtgaccac agtgacaaat ctcttcgaga agtggaacga
     3001 ctgcgagcga accgtcgtga tgtacgcgct gctcaagcgg ctgcgctacc cgagcctcaa
     3061 gtttctacag tattctatcg acagcaacct gacccagaac ctgggcacct cgcagaccaa
     3121 tctgagcagc gtggttattg acatcaatgc caacaatccg gtgtacctgc agaatctact
     3181 caacgcctac aagacgttcc agccatgtga cctgctcgac gccatgtcct cctcgtcgtc
     3241 cgacaaggac tcgatgccat gttacggcag tgacttccag atcaccacat cagcgcagtg
     3301 tgacgagcga aagctgtatg cccgcaagga ggacattctg catgaagtac tgaacatgct
     3361 gcctctgctg aagccgggca atgaggaggc caagcttatc tacctgaccc tcataccagt
     3421 tgccgtcaag gacaccatgc agcaaattgt gcccacggag ttggtgcagc agatcttctc
     3481 gtacctactc atccatccag ctatcaccag cgaggacaga cgttcgctca acatttggct
     3541 gcgtcacttg gaggatcata tccaagcggc tgcggcgggc ctgacaaatc gcagttactt
     3601 cctgcagccc tcgccgcaac tggtcgctgg tggtagctca acaggcagtg gtagctgttc
     3661 ctcttcggcg accagctctt cgacagcatc ctgttcgtcg gtggcctcat cctcgttgtg
     3721 ccccgcaagc ggcagccgtt cctcacgcac caacgactgg caaacgatcg ccccgcccag
     3781 caagcaattg caaaacaaat tggccggtga ttggcgtggc aatggaggcg gcagctccag
     3841 cgggtcgatc aatccactct gtgataatct taatggtatt accctgaacg aattagcttc
     3901 tagtcagaac agtctcggcc tgtcgctgga gggcagctct tccctggtca acggtgtggt
     3961 cgctggtgca ggctctatgt tgggaatcgc tggtggcgac gatcatgaca cgtcatttag
     4021 caagaatggc acggagattc tcgattttga cccggtgacg gcggacatgg gtgaggcctg
     4081 tagtctggcc agcagtagcc tttgtggacg taacggtgga aatcctgttg aggatcgatc
     4141 gcaacctccg ccgaatcttc agcagcaatt gcttcagcct cctccctatg cctcgatcct
     4201 tatgggaaac gtgggcgatc agtttggaga gatcaaccgg tggagcctgg acagcaagat
     4261 tgcagccctg aagacgcggc gctctaacag cctcaccacg caaacgatct ctagctgttc
     4321 cagctcgtct aactcatcgg tgatcacggt caatgacaac tgctccaatt caacggagaa
     4381 tctggcacag tttgccaaca agccccgtag tttttcctta tcgattgagc atcagcgcgg
     4441 cgccttgatg aacagcggtt ccgatactcg tctggatgaa cgctcgagtc tagagggccc
     4501 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
     4561 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctaa
     4621 ggcctgagct cgctgatcag cctcgatcga ggatccagac atgataagat acattgatga
     4681 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     4741 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     4801 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     4861 cctctacaaa tgtggtatgg ctgattatga tcagtcgacc gatgcccttg agagccttca
     4921 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     4981 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
     5041 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
     5101 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
     5161 aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga
     5221 cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc
     5281 ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag
     5341 gatcgctcgc ggctcttacc agccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5401 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5461 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5521 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5581 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     5641 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5701 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5761 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5821 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     5881 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5941 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6001 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6061 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6301 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6481 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6781 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6841 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6901 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6961 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7021 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7081 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7141 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     7201 ataggcgtat cacgaggccc tttcgt
//
