LOCUS       sequence-288460-        7928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmSmaug-del584-762.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7928)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmSmaug-del_584-762-V5His6_C
FEATURES             Location/Qualifiers
     source          1..7928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5213..5254
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5264..5281
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5304..5321)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5354..5488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5378..5397)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5432..5451
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6110..6698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6190..6209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6869..7660,7661..7729))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7492..7511
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7730..7834)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7902..7920
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catgaagtac gcaactggaa ctgacaacgc
     2761 gatgacctcc ggcatcagcg gccaaaccaa taactctaac agtgcatcca acgagatgca
     2821 gccaaccaca tcaacgccta ctgcagccca caaggaggcc acatcaacgg ccacgacgac
     2881 ggcgacatac gccaatggca atcccaattc taacgccaat cccagccaga gccagccgtc
     2941 caatgcgctg ttttgcgagc aggtgaccac agtgacaaat ctcttcgaga agtggaacga
     3001 ctgcgagcga accgtcgtga tgtacgcgct gctcaagcgg ctgcgctacc cgagcctcaa
     3061 gtttctacag tattctatcg acagcaacct gacccagaac ctgggcacct cgcagaccaa
     3121 tctgagcagc gtggttattg acatcaatgc caacaatccg gtgtacctgc agaatctact
     3181 caacgcctac aagacgttcc agccatgtga cctgctcgac gccatgtcct cctcgtcgtc
     3241 cgacaaggac tcgatgccat gttacggcag tgacttccag atcaccacat cagcgcagtg
     3301 tgacgagcga aagctgtatg cccgcaagga ggacattctg catgaagtac tgaacatgct
     3361 gcctctgctg aagccgggca atgaggaggc caagcttatc tacctgaccc tcataccagt
     3421 tgccgtcaag gacaccatgc agcaaattgt gcccacggag ttggtgcagc agatcttctc
     3481 gtacctactc atccatccag ctatcaccag cgaggacaga cgttcgctca acatttggct
     3541 gcgtcacttg gaggatcata tccaagcggc tgcggcgggc ctgacaaatc gcagttactt
     3601 cctgcagccc tcgccgcaac tggtcgctgg tggtagctca acaggcagtg gtagctgttc
     3661 ctcttcggcg accagctctt cgacagcatc ctgttcgtcg gtggcctcat cctcgttgtg
     3721 ccccgcaagc ggcagccgtt cctcacgcac caacgactgg caaacgatcg ccccgcccag
     3781 caagcaattg caaaacaaat tggccggtga ttggcgtggc aatggaggcg gcagctccag
     3841 cgggtcgatc aatccactct gtgataatct taatggtatt accctgaacg aattagcttc
     3901 tagtcagaac agtctcggcc tgtcgctgga gggcagctct tccctggtca acggtgtggt
     3961 cgctggtgca ggctctatgt tgggaatcgc tggtggcgac gatcatgaca cgtcatttag
     4021 caagaatggc acggagattc tcgattttga cccggtgacg gcggacatgg gtgaggcctg
     4081 tagtctggcc agcagtagcc tttgtggacg taacggtgga aatcctgttg aggatcgatc
     4141 gcaacctccg ccgaatcttc agcagcaatt gcttcagcct cctccctatg cctcgatcct
     4201 tatgggaaac gtgggcgatc agtttggaga gatcaaccgg tggagcctgg acagcaagat
     4261 tgcagccctg aagacgcggc gctctaacag cctcaccacg caaacgatct ctagctgttc
     4321 cagctcgtct aactcatcgg tgatcacggt caatgacaac tgctccaatt caacggagaa
     4381 tctggcacag tttgccaaca agccccgtag tttttcctta tcgattgagc atcagcgcgg
     4441 cgccttgatg aacagcggtt ccgatactcg tctggatgaa atgaagatct ccatggttcc
     4501 caaaatgcat cacgttaagc cagccggcgt cgggccaaac aatgggaaca ttaacaagcc
     4561 aagatggaat ggtaagactc gcaaatgcga tactaaaaat ggcagcaacg ataggattaa
     4621 caaccgcaaa aactccaacg acatgctaaa cttttcgcta aattgcctgc cacacccgct
     4681 gccccatcat tcgcagcagg ctccgccgcc tctgccacag tttgactaca acggctatgg
     4741 cggcggtcca tcccatcagc cgcagtacaa gagctcctcg tacccaagct tcatgggtaa
     4801 tcctcagcag cagccaccac cgccgccgtc tagtaagtcg catcatcatc cccagcagat
     4861 gcaacagatg ctgcagcaac acaaccactt tccagcgctg cctcagcaga cgccaccaca
     4921 atcgcatcgc cggtcgctga ataatcttat tctggtggcc ggaggtccgc agcaaccgca
     4981 gcaattgatc tttaagcccg gtcagggagt gctcaccaac aacggatcta acgacaacct
     5041 ggtattggaa cgcaatcagc agtcgcagca gcagagaaaa cttagcggag gagtgtccag
     5101 tgcggagcaa caaccaaaga agaccatggc cgctgttgtg atggaaaatt tggccaaatt
     5161 tgatcaacat tttacgctat tccgctcgag tctagagggc ccgcggttcg aaggtaagcc
     5221 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca
     5281 ttgagtttaa acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc
     5341 agcctcgatc gaggatccag acatgataag atacattgat gagtttggac aaaccacaac
     5401 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     5461 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     5521 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     5581 ggctgattat gatcagtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     5641 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     5701 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga
     5761 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag
     5821 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca
     5881 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct
     5941 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
     6001 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta
     6061 ccagccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6121 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6181 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6241 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6301 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6361 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6421 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6481 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6541 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6601 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6661 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6721 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6781 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6841 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6901 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6961 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7021 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7081 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7141 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     7201 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7261 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7321 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7381 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7441 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     7501 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7561 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7621 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7681 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7741 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7801 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7861 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     7921 cctttcgt
//
