LOCUS       sequence-288461-        8465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmSmaug-K606AR609A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8465)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmSmaug-K606AR609A-V5His6_C
FEATURES             Location/Qualifiers
     source          1..8465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5750..5791
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5801..5818
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5841..5858)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5891..6025
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5915..5934)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5969..5988
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6647..7235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6727..6746)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7406..8197,8198..8266))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8029..8048
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8267..8371)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8439..8457
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catgaagtac gcaactggaa ctgacaacgc
     2761 gatgacctcc ggcatcagcg gccaaaccaa taactctaac agtgcatcca acgagatgca
     2821 gccaaccaca tcaacgccta ctgcagccca caaggaggcc acatcaacgg ccacgacgac
     2881 ggcgacatac gccaatggca atcccaattc taacgccaat cccagccaga gccagccgtc
     2941 caatgcgctg ttttgcgagc aggtgaccac agtgacaaat ctcttcgaga agtggaacga
     3001 ctgcgagcga accgtcgtga tgtacgcgct gctcaagcgg ctgcgctacc cgagcctcaa
     3061 gtttctacag tattctatcg acagcaacct gacccagaac ctgggcacct cgcagaccaa
     3121 tctgagcagc gtggttattg acatcaatgc caacaatccg gtgtacctgc agaatctact
     3181 caacgcctac aagacgttcc agccatgtga cctgctcgac gccatgtcct cctcgtcgtc
     3241 cgacaaggac tcgatgccat gttacggcag tgacttccag atcaccacat cagcgcagtg
     3301 tgacgagcga aagctgtatg cccgcaagga ggacattctg catgaagtac tgaacatgct
     3361 gcctctgctg aagccgggca atgaggaggc caagcttatc tacctgaccc tcataccagt
     3421 tgccgtcaag gacaccatgc agcaaattgt gcccacggag ttggtgcagc agatcttctc
     3481 gtacctactc atccatccag ctatcaccag cgaggacaga cgttcgctca acatttggct
     3541 gcgtcacttg gaggatcata tccaagcggc tgcggcgggc ctgacaaatc gcagttactt
     3601 cctgcagccc tcgccgcaac tggtcgctgg tggtagctca acaggcagtg gtagctgttc
     3661 ctcttcggcg accagctctt cgacagcatc ctgttcgtcg gtggcctcat cctcgttgtg
     3721 ccccgcaagc ggcagccgtt cctcacgcac caacgactgg caaacgatcg ccccgcccag
     3781 caagcaattg caaaacaaat tggccggtga ttggcgtggc aatggaggcg gcagctccag
     3841 cgggtcgatc aatccactct gtgataatct taatggtatt accctgaacg aattagcttc
     3901 tagtcagaac agtctcggcc tgtcgctgga gggcagctct tccctggtca acggtgtggt
     3961 cgctggtgca ggctctatgt tgggaatcgc tggtggcgac gatcatgaca cgtcatttag
     4021 caagaatggc acggagattc tcgattttga cccggtgacg gcggacatgg gtgaggcctg
     4081 tagtctggcc agcagtagcc tttgtggacg taacggtgga aatcctgttg aggatcgatc
     4141 gcaacctccg ccgaatcttc agcagcaatt gcttcagcct cctccctatg cctcgatcct
     4201 tatgggaaac gtgggcgatc agtttggaga gatcaaccgg tggagcctgg acagcaagat
     4261 tgcagccctg aagacgcggc gctctaacag cctcaccacg caaacgatct ctagctgttc
     4321 cagctcgtct aactcatcgg tgatcacggt caatgacaac tgctccaatt caacggagaa
     4381 tctggcacag tttgccaaca agccccgtag tttttcctta tcgattgagc atcagcgcgg
     4441 cgccttgatg aacagcggtt ccgatactcg tctggatgaa ttcaagccca attatattaa
     4501 gttccacacg cgcaacgtgg gcatgtcggg aatcggactc tggctagcat ccttggcact
     4561 gcacaagtac atcgagctgt tcaagaacat gacgtacgag gaaatgctct tgatcaccga
     4621 ggactttctg cagagtgttg gcgtcaccaa gggcgcctcc cacaagcttg ccctttgcat
     4681 tgataagctg aaggaacggg ccaatattct taaccgggta gaacaagaac tgctatcggg
     4741 tcaaatggag ctgagcacgg cggttgagga gctaacaaat attgtgctga caccaatgaa
     4801 gccactggag tctccaggac caccggagga aaacattggt ctgcgttttc ttaaggtcat
     4861 tgacattgtg accaatacgt tgcagcagga tccgtatgct gtccaagacg acgagacttt
     4921 gggtgtgctt atgtggattc tagaccgttc tatccacaac gaggcattta tgaatcatgc
     4981 cagtcaactc aaggacctga agtttaagtt gtccaagatg aagatctcca tggttcccaa
     5041 aatgcatcac gttaagccag ccggcgtcgg gccaaacaat gggaacatta acaagccaag
     5101 atggaatggt aagactcgca aatgcgatac taaaaatggc agcaacgata ggattaacaa
     5161 ccgcaaaaac tccaacgaca tgctaaactt ttcgctaaat tgcctgccac acccgctgcc
     5221 ccatcattcg cagcaggctc cgccgcctct gccacagttt gactacaacg gctatggcgg
     5281 cggtccatcc catcagccgc agtacaagag ctcctcgtac ccaagcttca tgggtaatcc
     5341 tcagcagcag ccaccaccgc cgccgtctag taagtcgcat catcatcccc agcagatgca
     5401 acagatgctg cagcaacaca accactttcc agcgctgcct cagcagacgc caccacaatc
     5461 gcatcgccgg tcgctgaata atcttattct ggtggccgga ggtccgcagc aaccgcagca
     5521 attgatcttt aagcccggtc agggagtgct caccaacaac ggatctaacg acaacctggt
     5581 attggaacgc aatcagcagt cgcagcagca gagaaaactt agcggaggag tgtccagtgc
     5641 ggagcaacaa ccaaagaaga ccatggccgc tgttgtgatg gaaaatttgg ccaaatttga
     5701 tcaacatttt acgctattcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
     5761 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg
     5821 agtttaaacc cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc
     5881 ctcgatcgag gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     5941 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     6001 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     6061 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     6121 tgattatgat cagtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     6181 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     6241 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
     6301 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
     6361 tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
     6421 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
     6481 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
     6541 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca
     6601 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6661 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6721 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6781 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6841 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6901 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6961 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7021 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7081 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7141 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7201 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7261 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7321 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7381 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7441 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7501 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7561 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7621 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7681 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     7741 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7801 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7861 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7921 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7981 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     8041 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8101 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8161 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8221 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8281 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8341 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8401 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     8461 ttcgt
//
