LOCUS       sequence-28847-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Hosokawa N, Hara T, Kaizuka T, Kishi C, Takamura A, Miura Y, Iemura 
            S, Natsume T, Takehana K, Yamada N, Guan JL, Oshiro N, Mizushima N
  TITLE     Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 
            complex required for autophagy.
  JOURNAL   Mol Biol Cell. 2009 Apr . 20(7):1981-91.
  PUBMED    19211835
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2009 Apr . 20(7):1981-91."
COMMENT     Sequence Label: p3xFLAG-CMV10-hAtg13
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..102
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gcagagctcg tttagtgacc gtcagaatta accatggact acaaagacca tgacggtgat
       61 tataaagatc atgacatcga ttacaaggat gacgatgaca agcttgcggc cgcgaattca
      121 tcgatagatc tgatatcggt accagtcgac tctagaggat ccatggaaac tgatctcaat
      181 tcccaggaca gaaaggacct ggacaagttt attaaatttt ttgccctcaa gactgtccaa
      241 gtgattgtcc aggctcggct tggtgaaaag atttgcactc gttcatcatc ttctccaacg
      301 ggttcagatt ggttcaactt agcaatcaaa gacatcccag aggttacaca tgaagcaaag
      361 aaggcactgg caggacagct gcctgcagtc gggaggtcca tgtgtgtgga gatttcactt
      421 aagacttctg agggagattc catggagctg gaaatatggt gtcttgaaat gaatgaaaag
      481 tgtgataaag aaatcaaagt ttcctacacg gtgtacaaca gactgtcatt gctgctgaag
      541 tcccttcttg ctataactag ggtgacacca gcctataggc tctccaggaa acaagggcat
      601 gaatatgtca tattatacag gatatatttt ggagaagttc agctgagtgg cttaggagaa
      661 ggcttccaga cagttcgtgt tggggacagt gggcacccct gtgggcacca tcactctttc
      721 ttgtgcttac agaattaact tggcattcat gtctaccagg caatttgaga ggaccccacc
      781 tatcatgggg attattattg atcacttttg tggaccgtcc ctatcccagc tcctctccca
      841 tgcacccc
//
