LOCUS       sequence-288488-        8002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmTral-del46-60.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   Tritschler F, Eulalio A, Helms S, Schmidt S, Coles M, Weichenrieder 
            O, Izaurralde E, Truffault V
  TITLE     Similar modes of interaction enable Trailer Hitch and EDC3 to 
            associate with DCP1 and Me31B in distinct protein complexes.
  JOURNAL   Mol Cell Biol. 2008 Nov . 28(21):6695-708.
  PUBMED    18765641
REFERENCE   2  (bases 1 to 8002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2008 Nov . 28(21):6695-708."
COMMENT     Sequence Label: pAc5.1B-EGFP-DmTral-del46-60_C
FEATURES             Location/Qualifiers
     source          1..8002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5287..5328
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5338..5355
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5378..5395)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5428..5562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5452..5471)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5506..5525
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6184..6772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6264..6283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6943..7734,7735..7803))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7566..7585
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7804..7908)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7976..7994
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgaattcta tgagcggggg attaccggag ttgggctcca aaatcagcct
     3361 catttccaag gcggacattc gatacgaggg ccgcctgtac acggtggatc cgcaggagtg
     3421 caccattgcg ctctcgagtg tgcgatcctt cggtggctcc ggcggctccg gcatctacga
     3481 ctacatccta ttccgtggct cggacatcaa ggacattcgc gttgtcaaca accacacgct
     3541 gccgcaccac aatgacccgg cgatcatgca ggcgcagctc cagaacggac cgccccagat
     3601 gccgcagcac ttccccatgc ccagcgggat gagcggtccg ccgcagcagc aacaggtgcc
     3661 gtcgcagcaa ccgccccaga tgccaagcgg tggaggcagt ggtggtgctg gtggagcagg
     3721 agctcctggc ggcggcggac ccggctacgg caatggcaat ccgttcggca acctcggcgg
     3781 tcccaatctg gccaacatgg tgggcaatgc gggcggatcg cttgcacccg gttccggtgc
     3841 tccgggcagc ggacccttca tgcacatcgg tggcaatcag cagcaaccga agccccagca
     3901 gcagaaacag ccaaacatgc tggccggtgc ctcgcgatcg acgacgccca ttagcctgat
     3961 tgtgagtccc acggcggagc tgactcagca gcagcagctg caccagcaac agaacgctgg
     4021 ccagggtggc aacggacgcg atgcaggaca taagcgccag aaccaccagc agcaacagaa
     4081 tcagcagcat cagcaacgtg gcggacccag ccacaacaat atgcagcatc agcagcaacg
     4141 aggcggcggc agtggaacgg acttctataa ccagcagcgg gatcgtcggg actccggacg
     4201 tcaaatggac aacaactata gcaacaacta caacaacaat aataataatc agcgcaatcg
     4261 cggcggcggc aacggaatgc aacagcagca gcgaggagga aacggcggca gcggcggcgg
     4321 cggtggaaac ggaggtggaa acaacccggc ctggaacatg catcgcggca accagaactc
     4381 gaacaacatg atgaacatgc gcaaccgcgg catgggatcc cgcggcccca tgcgacccaa
     4441 tcagggctat cgtccgcagt cggccaataa tcagaacaag ccgcggaaca agatcaagtt
     4501 cgagggcgac ttcgatttcg agcaggcaaa caacaagttc gaggaactgc gctcccaact
     4561 ggccaagctc aaggtggccg aggatggtgc acccaagcca gccaccaatg caacggccgc
     4621 cactgcaact gcaaccaatg agcaggtggg tgagaaggtt gaaggcgttc acacactgaa
     4681 tggcgagacc gacaagaagg atgattctgg caacgagacc ggcgctggag agcacgagcc
     4741 tgaggaggat gatgttgctg tgtgctacga caagaccaaa tcgttcttcg acaacatctc
     4801 gtgcgaggct gcccaggatc gcagcaagaa caagaagaac gattggcgcc aggagcgcaa
     4861 gttgaacacg gagaccttcg gagtgtcctc cacacgacgt ggcagttacc gcggacgcaa
     4921 ccaatactac aacaataatg gcaacggcgg cattaactcg ggatatggag gagcgccagg
     4981 ctacaacagg aacaattatc gcatgggtgg cggcggcgga aacttccgaa acaggagcaa
     5041 caatcgcaac aacggcggcg gtcgtggcgg aaacggaatg ccaaacatca ccaatggcaa
     5101 cacggctgct gcgctgaagg cggccaacaa tgctgctggc cacggatcca atgccacgga
     5161 ctccagtgca ccaaatgcca caaccgcgac gacaaagtcg acgtccctct tgccagagca
     5221 gacgcaacag gtggcggcag tttcacaata ggcggccgct cgagtctaga gggcccgcgg
     5281 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat
     5341 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctaaggcc
     5401 tgagctcgct gatcagcctc gatcgaggat ccagacatga taagatacat tgatgagttt
     5461 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     5521 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     5581 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     5641 tacaaatgtg gtatggctga ttatgatcag tcgaccgatg cccttgagag ccttcaaccc
     5701 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     5761 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga
     5821 ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
     5881 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc
     5941 cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg
     6001 aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
     6061 gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc
     6121 gctcgcggct cttaccagcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6181 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6241 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6301 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6361 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6421 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6481 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6541 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6601 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     6661 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6721 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6781 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6841 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6901 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6961 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7021 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7081 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7141 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7201 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc
     7261 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7321 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7381 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7441 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7501 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
     7561 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7621 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7681 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7741 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7801 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7861 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7921 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     7981 gcgtatcacg aggccctttc gt
//
