LOCUS       sequence-288509-        8881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmCup.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Tritschler F, Eulalio A, Helms S, Schmidt S, Coles M, Weichenrieder 
            O, Izaurralde E, Truffault V
  TITLE     Similar modes of interaction enable Trailer Hitch and EDC3 to 
            associate with DCP1 and Me31B in distinct protein complexes.
  JOURNAL   Mol Cell Biol. 2008 Nov . 28(21):6695-708.
  PUBMED    18765641
REFERENCE   2  (bases 1 to 8881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2008 Nov . 28(21):6695-708."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmCup_C
FEATURES             Location/Qualifiers
     source          1..8881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6166..6207
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6217..6234
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6257..6274)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6307..6441
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6331..6350)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6385..6404
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7063..7651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7143..7162)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7822..8613,8614..8682))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8445..8464
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8683..8787)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8855..8873
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catggaccct atgaaagagg aggcgatcga
     2761 attgaatggc catgctatgc aaatggccga agctgagcaa gaaaatggcg ctggcgccct
     2821 gaaaattgcc accaatgcgg gtgcaaccga tcgtccagcg catcagcagc ttccgctgcc
     2881 agtcgaagat cagcaggatg aggtgctgac ccccgctgag aagggcaagt tcgagtaccc
     2941 gccccctcca ccgccaccaa ctccagtcca ggctccgcta gccaccaagg ccaccgctct
     3001 gaatgccagt caagagcatg acgacgacga ggccaactcc gagaagtggg aggatccgtg
     3061 cgctccgccc ccgcctcctc cgctgccgac tagcgctttc ctggccaccg gactgggcta
     3121 cctcaagctg cccgccttca agctgaagga cgcactggag aaggccatca ccaagttgga
     3181 ggccaacaag cggacgctaa aggccagccc tgagtcttct cgttccatca aaaacaagaa
     3241 tgtggtggcc ctggagatgt tacccaggcg ctcaaaccca gaaacgatcg gcgatggatc
     3301 catgttggca tccacatcca cggctgttat gctgcagacc aaaaagcccg ccgttattgt
     3361 ggagatggag aggcgttgta agatcatcaa tttactggcc aaacagaatc aaatcctcga
     3421 atccatttcg ggggaggcca ttccgatgca tggaccctcg aagcatctgc acgaggacga
     3481 gggtctgacc ctgcaagttc tctcggcccg agcatccaca ccgtacacac agccctccag
     3541 catgttgtcc tgcacggccg ttagttgtga cctggagcac gattcgcccc ggaagcaagt
     3601 ggccagcaag gaggccgtgc cggagcagca gtctagtcag gtgcagcaaa agcgtccacc
     3661 cagcacgggc attcacaagc caggaagtct gcgagcaccc aaggctgttc gtcctactac
     3721 tgctccggtg gtcagcagca agccggtcaa gagctatacc cgctcccgcc tgatggacat
     3781 tcgcaacggg atgttcaatg ccctgatgca caggtccaag gagagctttg tgatgcctcg
     3841 catcgccacc tgcgacgaca ttgagttgga gggaaggctt cgccgcatga atatttggcg
     3901 cacctcggac ggcacaagat tccggacccg ctccaccaca gctaacttaa acatgaacaa
     3961 caacaacaac aatgagtgca tgcctgcctt cttcaaaaac aaaaataagc cgaacctaat
     4021 cagtgacgag tccatcatcc agagccaacc gccccagccg caaacggagt tccaagatcc
     4081 ggccattgtt aaccaaagac gcattggcag cggtcgctta aaccattcca agtggggtta
     4141 caacgacgaa gactaccact cgtaccacaa tggcaagtcg cagcatatgg aggaggtcaa
     4201 ctcgaagaac agcaaaaaca tgacggtgct gcagttcttc gacaacggcg agatcagcag
     4261 tcagcctcaa agacggccga acactcctgt gatggggatg tctatcaatc gatccgagaa
     4321 cgacaccctg cactcgaacg agtccagtga agatttgagt cgcgccaacg agaactatgt
     4381 aaagcgcgta atgtctggat tcctggttgt atcgaagccc aagtcacgag atgtcgagga
     4441 tagacatcac cggcggtaca ggaaccagaa cgaggagcct gagtggttta gctgtggacc
     4501 cacgtctaga ctggacacta tagagttgtg tgggttcgat gaggacgagg agaaaatgct
     4561 aaaggagggc aacaaaaacc acggattagg cgaaacagag cgagaaacgt caaaacaaaa
     4621 gatggaccat aagtacaaat ggacgcatgc ggagccgatg ggtcgaagca aatacatgcc
     4681 caaacacgac actaacaata atcataacgt ggagaacatg aataatgtta tggctaccga
     4741 gcatcagcaa caaaaggaag agaaacgccc aggcagtggc cgttcgtttc agtttgataa
     4801 gttcaatcaa agccaacaga attacgagag tagcagttat gttaatcacc aacagccgcc
     4861 gcaaacgcag cctcagcaaa tgcagcagca atcgaacaca aacacgaaca actctaaatt
     4921 catgtcattc tttgccaatg aggggaactc ctcgtcttct tcgctgaacg agtttttcaa
     4981 gcaggccatt aaccagggtc atggcaacaa tccggagcag cccaaatcgt tgggccacat
     5041 cggacagatg ccttcggtgg atcagctgga ggccaaatgg cgtcgaaact cactgaacaa
     5101 tgttggcgag actgccaaca agcagactga taacttccag aagctaattg ggtctctttc
     5161 ttcggctaag ccacagtcac aggcggttgg ctatgatgcc atctccaact ttataatgca
     5221 gcaacagcag taccagcagc agcaacagaa gcagcacctc atcatacagc agcagcagca
     5281 acagaccgcc tttttggcga gcctgcagct gaaggcgatc ctcggccggg ccgacaccca
     5341 attgctactg ctccgtctga ctaaagggga aatatccaag cacggattgc tggtgcagtt
     5401 ggccaaccca cgcttgacgg acatggatcg cgaggccatc actgctgtat tgcagttcac
     5461 caacacccag cagcagcagc agcagcacaa gcagcagctg gacatgctct caagcacggt
     5521 catcgccagc cagttgcaga atctccacaa cttggccatt gtccagcaga ctcttgcagc
     5581 caggcagcag ccgcagcata atccacagac gcaggcgccg caccaactgt cgcaggagga
     5641 cttgcaagcg catgccaatg tgataatgcg aaacgccgta atgaagcgaa aaattgagga
     5701 gcagacctcg aagcttatca atggcggtgc caagcatcag gcacagcagc aatatctaaa
     5761 ccgtggccag caacgccagg ctcgaccaga tgccaactcg aatgccttgc tccatgcctt
     5821 gatctctggc ggtggcaata accacgcctc tggttatcct atgaaaggac agccgcagca
     5881 gcatcattcc aatttgagat tcggtgataa ccaaaacttc caatcttttg agtccaacca
     5941 gccgcacttc gccacgcagt acaagcagca ataccaacag tctcagcagc aacatcccca
     6001 tcagcagccc caacagctga attcgcttca tcagaacaac gctggagctg tcaatagctt
     6061 caataaggcc caaatgcaag ctcaatcagc tatttccatg ctgcccaaca gcggggatga
     6121 gtttcattaa gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct
     6181 aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt
     6241 taaacccgct gatcagcctc gactgtgcct tctaaggcct gagctcgctg atcagcctcg
     6301 atcgaggatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     6361 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     6421 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     6481 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     6541 tatgatcagt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     6601 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     6661 acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac
     6721 gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt
     6781 cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc
     6841 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat
     6901 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag
     6961 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcca
     7021 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7081 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7141 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7201 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     7261 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7321 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7381 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7441 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7501 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7561 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7621 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7681 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7741 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7801 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7861 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7921 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7981 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8041 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8101 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     8161 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8221 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8281 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8341 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8401 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     8461 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8521 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8581 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8641 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8701 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8761 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     8821 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     8881 t
//
