LOCUS       sequence-288528-        9560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsAGO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 9560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsAGO1_C
FEATURES             Location/Qualifiers
     source          1..9560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1197..1223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     polyA_signal    5264..5385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5275..5294)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5329..5348
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5571..6026
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5658..5677)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5868..5889
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6143..6500
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6143..6334
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6166..6186)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6351..6486
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6551..7345
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6605..6624)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7215..7234
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7409..7457
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7498..7517)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7617..7639
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7677..7695)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7763..7867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7868..7936,7937..8728)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8086..8105)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8899..9487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9388..9407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg aggctgccca agcttacacc atggcctacc
     1201 catatgatgt tccagattac gcttcactcg acgcggatcc cgtcgaattc atggaagcgg
     1261 gaccctcggg agcagctgcg ggcgcttacc tgccccccct gcagcaggtg ttccaggcac
     1321 ctcgccggcc tggcattggc actgtgggga aaccaatcaa gctcctggcc aattactttg
     1381 aggtggacat ccctaagatc gacgtgtacc actacgaggt ggacatcaag ccggataagt
     1441 gtccccgtag agtcaaccgg gaagtggtgg aatacatggt ccagcatttc aagcctcaga
     1501 tctttggtga tcgcaagcct gtgtatgatg gaaagaagaa catttacact gtcacagcac
     1561 tgcccattgg caacgaacgg gtcgactttg aggtgacaat ccctggggaa gggaaggatc
     1621 gaatctttaa ggtctccatc aagtggctag ccattgtgag ctggcgaatg ctgcatgagg
     1681 ccctggtcag cggccagatc cctgttccct tggagtctgt gcaagccctg gatgtggcca
     1741 tgaggcacct ggcatccatg aggtacaccc ctgtgggccg ctccttcttc tcaccgcctg
     1801 agggctacta ccacccgctg gggggtgggc gcgaggtctg gttcggcttt caccagtctg
     1861 tgcgccctgc catgtggaag atgatgctca acattgatgt ctcagccact gccttttata
     1921 aggcacagcc agtgattgag ttcatgtgtg aggtgctgga catcaggaac atagatgagc
     1981 agcccaagcc cctcacggac tctcagcgcg ttcgcttcac caaggagatc aagggcctga
     2041 aggtggaagt cacccactgt ggacagatga agaggaagta ccgcgtgtgt aatgttaccc
     2101 gtcgccctgc tagccatcag acattcccct tacagctgga gagtggacag actgtggagt
     2161 gcacagtggc acagtatttc aagcagaaat ataaccttca gctcaagtat ccccatctgc
     2221 cctgcctaca agttggccag gaacaaaagc atacctacct tcccctagag gtctgtaaca
     2281 ttgtggctgg gcagcgctgt attaaaaagc tgaccgacaa ccagacctcg accatgataa
     2341 aggccacagc tagatccgct ccagacagac aggaggagat cagtcgcctg atgaagaatg
     2401 ccagctacaa cttagatccc tacatccagg aatttgggat caaagtgaag gatgacatga
     2461 cggaggtgac agggcgagtg ctgccggcgc ccatcttgca gtacggcggc cggaaccggg
     2521 ccattgccac acccaatcag ggtgtctggg acatgcgggg gaaacagttc tacaatggga
     2581 ttgagatcaa agtctgggcc atcgcctgct tcgcacccca aaaacagtgt cgagaagagg
     2641 tgctcaagaa cttcacagac cagctgcgga agatttccaa ggatgcgggg atgcctatcc
     2701 agggtcaacc ttgtttctgc aaatatgcac agggggcaga cagcgtggag cctatgttcc
     2761 ggcatctcaa gaacacctac tcagggctgc agctcattat tgtcatcctg ccagggaaga
     2821 cgccggtgta tgctgaggtg aaacgtgtcg gagatacact cttgggaatg gctacgcagt
     2881 gtgtgcaggt gaagaacgtg gtcaagacct cacctcagac tctgtccaac ctctgcctca
     2941 agatcaatgt caaacttggt ggcattaaca acatcctagt cccacaccag cgctctgccg
     3001 tttttcaaca gccagtgata ttcctgggag cagatgttac acacccccca gcaggggatg
     3061 ggaaaaaacc ttctatcaca gcagtggtag gcagtatgga tgcccacccc agccgatact
     3121 gtgctactgt gcgggtacag cgaccacggc aagagatcat tgaagacttg tcctacatgg
     3181 tgcgtgagct cctcatccaa ttctacaagt ccacccgttt caagcctacc cgcatcatct
     3241 tctaccgaga tggggtgcct gaaggccagc taccccagat actccactat gagctactgg
     3301 ccattcgtga tgcctgcatc aaactggaaa aggactacca gcctgggatc acttatattg
     3361 tggtgcagaa acgccatcac acccgccttt tctgtgctga caagaatgag cgaattggga
     3421 agagtggtaa catcccagct gggaccacag tggacaccaa catcacccac ccatttgagt
     3481 ttgacttcta tctgtgcagc cacgcaggca tccagggcac cagccgacca tcccattact
     3541 atgttctttg ggatgacaac cgtttcacag cagatgagct ccagatcctg acgtaccagc
     3601 tgtgccacac ttacgtacga tgcacacgct ctgtctctat cccagcacct gcctactatg
     3661 cccgcctggt ggctttccgg gcacgatacc acctggtgga caaggagcat gacagtggag
     3721 aggggagcca catatcgggg cagagcaatg ggcgggaccc ccaggccctg gccaaagccg
     3781 tgcaggttca ccaggatact ctgcgcacca tgtacttcgc ttgaaggcag aacgctgtta
     3841 cctcactgga tagaagaaag ctttccaagc cccaggagct gtgccaccca aatccagagg
     3901 aagcaaggag gagggaggtg gggtagggag gagtgtagga tgccttgttt ccttctatag
     3961 aggtggtgta agagtgggga acagggccag caagacagac caccagccag aaatctctga
     4021 tatcaacctc atgtccccca cccctcaccc catcttgtca catctggccc tgaccccact
     4081 ggaccaaaag gggcagcact ggtgcccacc atacacacag gtgtctcatg tgactcacag
     4141 tgctaaagac tcatgcttga cagcttggta aggtcaactc tgtagccctg cagacaaaag
     4201 ctggttaggt ttgggtttga tactttagat gggaaagtga ggggcttgag aaagtgggtg
     4261 ggaggaggga aggatttttt aggagcctta atcagaaaag gactagattt gtttaagaag
     4321 aaaaatgaaa ccagacccag atcaatattt taggatacta gatgttttaa tgggttcaga
     4381 atccagtttg taggaagatt ttttaatggt tttggttgct cctcccccag ctgccacccc
     4441 ccaccttacc cttattcctc tctgtccaca ttttctgccc caccttactt ctcctccctg
     4501 acagacatcc agcccctagt aatacttaag gcactatggc acttagcttt gaagtgacac
     4561 gaccctgtct tccttccgcc cgctggtggg taaccagtgc cttccctgta acggtaatgc
     4621 tgcagaactg caaccttttg tacctttctt tggggaatgg ggtgggggtg ggagaggagg
     4681 tagatgggga agaaataccc cagacccaac aaacctccag ccagaaagcc agctattttg
     4741 catttgaagg aattgacttc ctcattcatt gagcttttta aaagatcaca acctcaagat
     4801 ggttaaaatc cattgacatt tgcactttca aacatgacaa gtctcggagc tgctgagatg
     4861 acaggcccct ggcctttcca cttatgcctc cttttctcct tattcctcct acctcccgcc
     4921 ccgcccaggt ctggagttac tttcatagca tttttcactc ttggcttctt ttctcccttg
     4981 atggtcaagt ctcttatgtt tcaatatttc ttaactgggg tgtcttataa caaaaaactc
     5041 ttaggtctaa aatgagaaaa aagagagaaa acaaaatgtt atttttatac cataacttga
     5101 gtgtattgcc aaaatttgga aatccttccc atgcctgatg agtttatatc ccagaaacat
     5161 tgagccatca gaatgaaaaa aaaaaaaaaa aggccacatg tgctcgagct gcaggtcgcg
     5221 gccgcttccc tttagtgagg gttaatgctt cgagcagaca tgataagata cattgatgag
     5281 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     5341 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     5401 attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag caagtaaaac
     5461 ctctacaaat gtggtaaaat ccgataagga tcgatccggg ctggcgtaat agcgaagagg
     5521 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg
     5581 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     5641 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     5701 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     5761 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     5821 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5881 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5941 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     6001 taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc
     6061 ggtatttcac accgcatacg cggatctgcg cagcaccatg gcctgaaata acctctgaaa
     6121 gaggaacttg gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     6181 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6241 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6301 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6361 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6421 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6481 gaggcctagg cttttgcaaa aagcttgatt cttctgacac aacagtctcg aacttaaggc
     6541 tagagccacc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     6601 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     6661 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     6721 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     6781 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     6841 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     6901 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     6961 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     7021 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     7081 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     7141 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     7201 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     7261 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     7321 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     7381 acgcccaacc tgccatcacg atggccgcaa taaaatatct ttattttcat tacatctgtg
     7441 tgttggtttt ttgtgtgaat cgatagcgat aaggatccgc gtatggtgca ctctcagtac
     7501 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     7561 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     7621 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     7681 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     7741 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7801 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7861 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7921 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7981 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8041 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8101 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8161 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8221 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8281 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8341 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8401 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8461 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8521 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8581 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8641 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8701 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8761 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8821 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8881 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8941 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9001 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9061 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9121 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9181 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9241 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9301 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9361 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9421 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9481 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9541 tcacatggct cgacagatct
//
