LOCUS       sequence-288532-       10741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6Ciso1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10741)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 10741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6Biso1_C
FEATURES             Location/Qualifiers
     source          1..10741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6445..6566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6456..6475)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6510..6529
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6752..7207
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6839..6858)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7049..7070
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7324..7681
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7324..7515
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7347..7367)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7532..7667
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7732..8526
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7786..7805)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8396..8415
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8590..8638
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8679..8698)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8798..8820
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8858..8876)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8944..9048
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9049..9117,9118..9909)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9267..9286)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10080..10668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10569..10588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg agaattcacg cgtggtacct ctagagatgg
     1201 cttacccata cgatgttcca gattacgcta tgagagaaaa ggagcaagaa agggaagaac
     1261 agttaatgga agacaagaaa aggaagaaag aggataaaaa gaaaaaagaa gccactcaga
     1321 aggtcacgga acaaaaaacc aaagtgcccg aagtgacgaa accaagttta agccaaccaa
     1381 cggccgccag cccaattggc agctctccat cgccaccagt caatggtggc aacaatgcca
     1441 aaagggtggc agtgccgaac ggacaaccgc caagcgccgc ccgctacatg cctcgggagg
     1501 tgccgccgcg attccgttgc cagcaggacc acaaagtgtt actaaaacgt gggcagcccc
     1561 ctccaccgtc ctgcatgctc cttgggggtg gggcagggcc tcctccctgc acagcacctg
     1621 gagcaaaccc aaacaacgca caagtgacag gagcgctgct gcagagtgag agtgggactg
     1681 cgccagactc aacccttgga ggtgctgctg cttcaaatta tgcaaattcc acttggggct
     1741 cgggagcctc ctccaacaac ggcacctccc ccaacccaat tcacatctgg gacaaggtga
     1801 ttgtagacgg gtctgacatg gaagagtggc cttgtattgc cagcaaagac actgaatctt
     1861 cttccgaaaa caccaccgat aacaacagtg cctcgaaccc tggctctgag aagagcactc
     1921 tgccaggaag caccactagt aacaaaggaa aagggagcca gtgccagtct gcaagttctg
     1981 ggaacgaatg taatcttggg gtctggaaat ctgaccctaa ggctaaatct gttcaatctt
     2041 ccaactctac tacagagaac aacaatggac taggaaattg gaggaatgtg agtggtcagg
     2101 atagaattgg acctggctct ggcttcagca actttaaccc aaatagcaac ccatctgcct
     2161 ggccagcact ggtccaagaa ggaacttcta ggaaaggggc attggaaaca gataatagta
     2221 attccagtgc acaggttggc acagtaggtc agacatccag ggaacagcag tcaaagatgg
     2281 aaaatgcggg tgttaatttt gttgtctctg gcagagaaca ggctcaaatt cataacactg
     2341 atggaccaaa aaatggaaac actaactcct tgaacttaag ttcaccaaac cccatggaga
     2401 ataagggaat gccctttgga atgggcttgg ggaacacctc caggagcact gatgcccctt
     2461 cacaaagcac tggagatcga aagactggga gtgttggatc ttggggtgca gctagggggc
     2521 cttctggaac tgacacagtc tctggacaaa gcaattctgg aaacaatggg aacaatggaa
     2581 aagagagaga ggactcctgg aaaggagctt ctgttcagaa atcaactggg tcaaaaaatg
     2641 actcttggga caacaataac aggtctacgg gtgggtcctg gaactttggc ccccaggact
     2701 ctaatgacaa caaatggggt gaagggaaca aaatgacatc tggggtctct cagggagaat
     2761 ggaaacagcc gactgggtct gatgagttga aaattggaga atggagtggt ccaaaccgac
     2821 caaattctag cactggagca tgggacaatc aaaagggcca ccccctccct gaaaaccaag
     2881 gcaatgccca ggctccctgt tggggaagat cttccagctc cacaggaagt gaagttggag
     2941 gtcaaagcac tggaagcaac cacaaagcag gaagtagtga cagtcataac tctggccgtc
     3001 ggtcgtacag gcccacacat cctgattgtc aggctgtctt gcagactctt ttgagccgaa
     3061 ctgatttgga ccccagggtg ctctcaaaca ctggctgggg ccaaactcaa attaagcagg
     3121 acacagtgtg ggacattgaa gaggtgccaa ggcctgaggg gaaatctgac aaaggaactg
     3181 aggggtggga gagcgctgcc acacagacca agaactcagg gggctgggga gatgcaccca
     3241 gccaaagcaa tcaaatgaag tctggatggg gggagctctc agcctctaca gagtggaaag
     3301 accccaagaa cacaggaggc tggaatgact acaagaacaa caactcttcc aactggggag
     3361 gaggacgacc tgatgaaaag acaccttcct cttggaatga gaatcccagc aaggatcagg
     3421 ggtggggagg tggacgccag cccaatcaag gatggtcttc tggaaagaat ggttgggggg
     3481 aggaagtcga tcagacaaaa aacagcaatt gggaaagttc tgcaagtaaa cctgtgtctg
     3541 ggtggggtga aggagggcag aatgaaatcg ggacttgggg taatggtggc aatgcaagcc
     3601 tagcttcaaa aggtgggtgg gaggattgca aaagatcccc agcatggaat gagacgggcc
     3661 gacagcccaa ttcctggaat aaacaacacc aacagcagca gcccccacag cagccgccgc
     3721 caccacaacc agaggcttct ggttcgtggg gaggcccacc cccaccacct ccaggcaacg
     3781 ttcgaccttc caattccagc tggagcagcg ggccacagcc tgcaacacct aaggatgagg
     3841 aacccagtgg ttgggaagag ccatccccac agtcaattag tcggaaaatg gacattgatg
     3901 atggcacttc agcatgggga gaccctaaca gttataacta caagaatgtg aatctgtggg
     3961 ataagaattc ccaagggggc ccagcacctc gagaaccaaa cctgcccacc ccaatgacca
     4021 gtaaatcggc atcagattcc aaatctatgc aagacggctg gggggagagt gacgggccag
     4081 tcacaggagc tcgccatccc agctgggaag aggaggagga tggaggagtc tggaacacca
     4141 ctggctctca gggcagtgct tcctcccaca actcagcaag ctggggacaa ggaggaaaga
     4201 aacaaatgaa gtgctcactc aaaggaggaa acaatgattc atggatgaat cctcttgcca
     4261 aacagttttc aaatatggga ttgctgagtc agactgaaga taatccaagc agcaaaatgg
     4321 atttgtctgt aggaagcctt tcagataaaa aatttgatgt ggacaagcga gcgatgaatc
     4381 tcggggattt taatgatatc atgaggaagg atcgatctgg gttccgtcca cctaattcca
     4441 aagacatggg aaccacagat agtgggcctt attttgagaa gggcggtagt catggtttgt
     4501 ttggaaacag cacagcacaa tcgagaggtc tgcacacacc cgtgcagcca ctaaattctt
     4561 ctcccagtct ccgggcgcaa gtgcctcccc agtttatttc cccccaggtt tctgcctcaa
     4621 tgctcaagca gtttcccaac agtggcctga gtccaggtct tttcaatgtg gggccccagt
     4681 tatctcctca acaaattgcc atgctgagcc agcttccaca aattccccag tttcagttgg
     4741 catgtcagct tctcttgcag cagcagcaac agcagcagtt gttacagaac cagagaaaga
     4801 tttctcaagc tgtacgccaa cagcaagagc agcagctggc tcgaatggtg agtgcactgc
     4861 agcagcagca gcagcagcag aggcagccag gcatgaagca ctcgccctct catcctgttg
     4921 ggcccaagcc gcatctggac aacatggtac ccaacgcatt gaatgtgggg ctcccagacc
     4981 ttcaaaccaa agggccaata cctggatatg gttctggctt cagctctggc ggcatggact
     5041 atggcatggt tggtgggaag gaggctggaa ccgagtctcg ctttaaacag tggacctcca
     5101 tgatggaggg gctgccctct gtagccacac aggaagccaa tatgcacaaa aatggcgcta
     5161 tagtggcccc tggtaaaacc cggggagggt caccgtacaa ccagtttgat atcatccctg
     5221 gtgacacact gggtggccat acgggtcctg ctggtgatag ctggttacct gccaaatctc
     5281 caccaacaaa taaaatcgga agtaaatcca gcaatgccag ttggcctcca gaattccaac
     5341 caggagtgcc atggaaaggt atccaaaaca ttgaccctga atctgacccc tatgtcaccc
     5401 caggaagtgt gctggggggt acagccacat ctcccattgt agatactgac caccaactgc
     5461 tgcgggataa caccacaggg tctaattctt ccctcagcac ctcgctgcct tcacctggtg
     5521 cctggcccta cagtgcctct gacaactcct ttaccaacgt tcatagcact tcagcaaagt
     5581 tccctgatta caaatcaaca tggtccccag atcccatagg acacaacccc actcatctct
     5641 ccaacaagat gtggaaaaac catatttcct ccaggaacac tacaccgctg ccccgcccac
     5701 ctcctggtct gaccaacccc aaaccatcat ctccctggag cagcacagca ccccgatcag
     5761 tcagggggtg ggggacacag gactcacggc tcgcctcggc ctctacctgg agtgatggtg
     5821 gctcagttcg tcctagttac tggctggttc ttcacaatct caccccacag attgatgggt
     5881 caaccttgag aacgatctgc atgcagcatg gcccactgct gacattccat ctgaatctaa
     5941 cccagggcac tgccctgatc cgatacagca ccaaacagga ggcggccaag gcccaaactg
     6001 cgctgcacat gtgtgtgttg ggaaacacta ccatccttgc tgagtttgcc actgatgatg
     6061 aagtcagccg ctttctggca caagctcagc cccctacacc tgcagcaacc ccaagtgcgc
     6121 cagctgcggg gtggcagtcg ctggagaccg gccagaacca gtcagatccc gtgggacctg
     6181 ctctgaatct ttttggtggg tccactgggc tcgggcagtg gagcagcagc gctggtggca
     6241 gcagcggggc cgatcttgct ggcgcttcat tgtgggggcc cccaaactat tcttctagct
     6301 tatggggagt cccaacggtg gaagatcccc ataggatggg cagccctgct cctttactac
     6361 ctggtgacct tctgggagga gggtcggatt caatctgagc ggccgcttcc ctttagtgag
     6421 ggttaatgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     6481 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     6541 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     6601 ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
     6661 tccgataagg atcgatccgg gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6721 ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg
     6781 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6841 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6901 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6961 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7021 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7081 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7141 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     7201 acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     7261 gcggatctgc gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac
     7321 cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7381 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     7441 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7501 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7561 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7621 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     7681 aaagcttgat tcttctgaca caacagtctc gaacttaagg ctagagccac catgattgaa
     7741 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     7801 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     7861 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     7921 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     7981 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     8041 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     8101 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     8161 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     8221 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     8281 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     8341 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     8401 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     8461 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     8521 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     8581 gatggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
     8641 tcgatagcga taaggatccg cgtatggtgc actctcagta caatctgctc tgatgccgca
     8701 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     8761 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     8821 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     8881 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     8941 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     9001 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     9061 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     9121 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     9181 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9241 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     9301 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     9361 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     9421 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     9481 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     9541 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     9601 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     9661 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     9721 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     9781 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     9841 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     9901 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     9961 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    10021 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10081 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10141 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    10201 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    10261 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    10321 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    10381 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10441 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    10501 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10561 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10621 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10681 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatggc tcgacagatc
    10741 t
//
