LOCUS       sequence-28854-e         594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 594)
  TITLE     Ghosh Lab Plasmids
REFERENCE   2  (bases 1 to 594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Myc-CREST (C88)
FEATURES             Location/Qualifiers
     source          1..594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        234..252
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     234..251
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     265..284
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      369..378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             378..407
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agctcgttta gtgancgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
       61 agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt
      121 ccccgtgcca agagtgacgt aagtaccgcc tatagagtct ataggcccac ccccttggct
      181 tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg
      241 tgacactata gaataacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc
      301 aactgcacct cggttctatc gattgaattc ccgaaccgac agtcggtctc ttcaccaagg
      361 ccattcgcgc caccatggag caaaagctca tttctgagga agatctcaat ggtggtggtg
      421 gtgggtcgac gatgtccgtg gccttcgcgt cggcgcggcc gagaggcaaa ggggaggtca
      481 ctcagcagac catccagaag atgctggatg agaaccacca cctgatccag tgcatcctgg
      541 actaccagag caagggcaag acagccgagt gcacccagta ccggcagatc ctgc
//
