LOCUS       sequence-28855-e         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  TITLE     Ghosh Lab Plasmids
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HA-CREST (C89)
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        218..236
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     218..235
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     249..268
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      353..362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             365..391
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
       61 gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt gccaagagtg
      121 acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta gaacgcggct
      181 acaattaata cataacctta tgtatcatac acatacgatt taggtgacac tatagaataa
      241 catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc acctcggttc
      301 tatcgattga attcccgaac cgacagtcgg tctcttcacc aaggccattc gcgccaccat
      361 gggctacccc tatgatgtgc ctgactacgc agatctcaat ggtggtggtg gtgggtcgac
      421 gatgtccgtg gccttcgcgt cggcgcggcc gagaggcaaa ggggaggtca ctcagcagac
      481 catccagaag atgctggatg agaaccacca cctgatccag tgcatcctgg actaccagag
      541 caagggcaag acagccgagt gcacccagta ccagcagatc ctgcaccgga acctggtcta
      601 cctggccacc a
//
