LOCUS       sequence-28858-e         576 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 576)
  TITLE     Ghosh Lab Plasmids
REFERENCE   2  (bases 1 to 576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRK5-HA-LMO4 (L4)
FEATURES             Location/Qualifiers
     source          1..576
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        236..254
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     236..253
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     267..286
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      371..380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             383..409
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagaca ccgggaccga tccagcctcc gcggccggga acggtgcatt ggaacgcgga
      121 ttccccgtgc caagagtgac gtaagtaccg cctatagagt ctataggccc acccccttgg
      181 cttcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta
      241 ggtgacacta tagaataaca tccactttgc ctttctctcc acaggtgtcc actcccaggt
      301 ccaactgcac ctcggttcta tcgattgaat tcccgaaccg acagtcggtc tcttcaccaa
      361 ggccattcgc gccaccatgg gctaccccta tgatgtgcct gactacgcag atctcaatgg
      421 tggtggtggt gggtcgacca tggtgaatcc gggcagcagc tcgcagccgc ccccggtgac
      481 ggccggctcc ctctcctgga agcgctgcgc aggctgcggg ggcaagattg cggaccgctt
      541 tctgctctat gccatggaca gctactggca cagccg
//
