LOCUS       sequence-28860-e         609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 609)
  TITLE     Ghosh Lab Plasmids
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Myc-ND2 (N72)
FEATURES             Location/Qualifiers
     source          1..609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        223..241
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     223..240
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     254..273
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      358..367
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             367..396
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gaaccgtcan atcgcctgga gacgccatcc acgctgtttt gacctcnata gaagacaccg
       61 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa
      121 gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt cgttagaacg
      181 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
      241 aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca actgcacctc
      301 ggttctatcg attgaattcc cgaaccgaca gtcggtctct tcaccaaggc cattcgcgcc
      361 accatggagc aaaagctcat ttctgaggaa gatctcaatg gtggtggtgg tgggtcgaca
      421 atgctgaccc gcctgttcag cgagcccggc ctcctctcgg acgtgcccaa gttcgccagc
      481 tggggcgacg gcgacgacga cgagccgagg agcgacaagg gcgacgcgcc gccgcagcct
      541 tctcctgctc ccggntcggg ggctccagga ccngccctgn ccgncangcc ngtggctctt
      601 cgtnnnagg
//
